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![Western Blot: WISP3 Antibody [NBP2-93872] - Analysis of extracts of various cell lines, using WISP3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/WISP3-Antibody-Western-Blot-NBP2-93872-img0001.jpg "Western Blot: WISP3 Antibody [NBP2-93872] - Analysis of extracts of various cell lines, using WISP3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: WISP3 Antibody [NBP2-56060] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/WISP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56060-img0001.jpg "Immunocytochemistry/Immunofluorescence: WISP3 Antibody [NBP2-56060] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum.")
![Immunocytochemistry/Immunofluorescence: WISP3 Antibody [NBP2-69068] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/WISP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-69068-img0001.jpg "Immunocytochemistry/Immunofluorescence: WISP3 Antibody [NBP2-69068] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum.")
![Western Blot: WISP3 Antibody [H00008838-B01P-50ug] - Expression in transfected 293T cell line. Lane 1: WISP3 transfected lysate(41.40 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/WISP3-Antibody-Western-Blot-H00008838-B01P-50ug-img0001.jpg "Western Blot: WISP3 Antibody [H00008838-B01P-50ug] - Expression in transfected 293T cell line. Lane 1: WISP3 transfected lysate(41.40 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: SH3PX1 Antibody (1B5) [NBP2-02609] Analysis of extracts (35ug) from 9 different cell lines by using anti-SH3PX1 monoclonal antibody.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Western-Blot-NBP2-02609-img0008.jpg "Western Blot: SH3PX1 Antibody (1B5) [NBP2-02609] Analysis of extracts (35ug) from 9 different cell lines by using anti-SH3PX1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3PX1.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Immunocytochemistry-Immunofluorescence-NBP2-02609-img0007.jpg)
![Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Human prostate tissue using anti-SH3PX1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Immunohistochemistry-Paraffin-NBP2-02609-img0006.jpg)
![Flow Cytometry: SH3PX1 Antibody (1B5) [NBP2-02609] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SH3PX1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Flow-Cytometry-NBP2-02609-img0001.jpg)
![Western Blot: SH3PX1 Antibody (1B5) [NBP2-02609] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3PX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3PX1.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Western-Blot-NBP2-02609-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody (1B5) [NBP2-02609] - Immunofluorescent staining of HeLa cells using anti-SH3PX1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Immunocytochemistry-Immunofluorescence-NBP2-02609-img0003.jpg)
![Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SH3PX1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Immunohistochemistry-Paraffin-NBP2-02609-img0004.jpg)
![Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Human colon tissue using anti-SH3PX1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-1B5-Immunohistochemistry-Paraffin-NBP2-02609-img0005.jpg)
![Western Blot: SH3PX1 Antibody [NBP1-80777] - Analysis in human cell lines SK-MEL-30 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Western-Blot-NBP1-80777-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SH3PX1 Antibody [NBP1-80777] - Analysis in human cell lines SK-MEL-30 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SH3PX1 Antibody [NBP1-80777] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Western-Blot-NBP1-80777-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody [NBP1-80777] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80777-img0008.jpg)
![Immunohistochemistry-Paraffin: SH3PX1 Antibody [NBP1-80777] - Staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Immunohistochemistry-Paraffin-NBP1-80777-img0011.jpg)
![Western Blot: SH3PX1 Antibody (3C6) [H00051429-M03] - Analysis of SNX9 expression in human liver.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-3C6-Western-Blot-H00051429-M03-img0004.jpg "Western Blot: SH3PX1 Antibody (3C6) [H00051429-M03] - Analysis of SNX9 expression in human liver.")
![Western Blot: SH3PX1 Antibody (3C6) [H00051429-M03] - Analysis of SNX9 expression in HeLa.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-3C6-Western-Blot-H00051429-M03-img0003.jpg)
![Western Blot: SH3PX1 Antibody (3C6) [H00051429-M03] - Analysis of SNX9 expression in transfected 293T cell line by SNX9 monoclonal antibody (M03), clone 3C6. Lane 1: SNX9 transfected lysate (Predicted MW: 66.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-3C6-Western-Blot-H00051429-M03-img0005.jpg)
![Immunoprecipitation: SH3PX1 Antibody (3C6) [H00051429-M03] - Analysis of SNX9 transfected lysate using anti-SNX9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SNX9 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-3C6-Immunoprecipitation-H00051429-M03-img0001.jpg)
![Sandwich ELISA: SH3PX1 Antibody (3C6) [H00051429-M03] - Detection limit for recombinant GST tagged SNX9 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-3C6-Sandwich-ELISA-H00051429-M03-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody [NB100-2813] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2813-img0001.jpg "Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody [NB100-2813] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Western Blot: SH3PX1 Antibody [NBP2-20348] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Western-Blot-NBP2-20348-img0001.jpg "Western Blot: SH3PX1 Antibody [NBP2-20348] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: SH3PX1 Antibody [NBP2-20348] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Immunohistochemistry-Paraffin-NBP2-20348-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody [NBP2-58144] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/SH3PX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58144-img0001.jpg "Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody [NBP2-58144] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.")