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Chromosome Separation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Chromosome Separation Pathway and Malignant Neoplasms, Neoplasms, Aneuploidy, Polyploidy, Cytogenetic Abnormality. The study of the Chromosome Separation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Chromosome Separation Pathway has been researched in relation to Anaphase, Mitosis, Metaphase, Cell Cycle, Cell Division. The Chromosome Separation Pathway complements our catalog of research reagents including antibodies and ELISA kits against SEPARASE, APC, CCNB1, CDK1, CDC20.

Top Research Reagents

We have 2638 products for the study of the Chromosome Separation Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

PLK1 Antibody (OTI1D4)
NBP1-48291
Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug ofImmunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IHC

     1 Review

ADCY10 Antibody
NBP1-81765
Immunohistochemistry-Paraffin: ADCY10 Antibody [NBP1-81765] - Staining of human kidney shows strong positivity in tubules.Immunohistochemistry-Paraffin: ADCY10 Antibody [NBP1-81765] - Staining of human pancreas shows strong membranous positivity in subset of exocrine glandular cells.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

CDC2/CDK1 Antibody
NBP1-85729
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

5 Publications
SGSM3 Antibody
NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

PTTG1 Antibody
NBP2-20287
Western Blot: PTTG1 Antibody [NBP2-20287] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: PTTG1 Antibody [NBP2-20287] - Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTTG1 protein stained by Securin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

SS18L1 Antibody
NBP2-20486
Western Blot: SS18L1 Antibody [NBP2-20486] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: SS18L1 Antibody [NBP2-20486] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

ERK1 Antibody (1E5) - BSA Free
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

1 Publication
Cdc20 Antibody
NB100-59828
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

6 Publications
MAD2L1 Antibody
NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

14 Publications
DNA Polymerase delta, catalytic subunit Antibody
NBP2-33466
Immunohistochemistry-Paraffin: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Analysis in human lymph node and liver tissues. Corresponding POLD1 RNA-seq data are presented for the same tissues.Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

Mesothelin Antibody (420411) [Unconjugated]
MAB32652
HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # <a class=Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # <a class=

Rat Monoclonal
Species Human
Applications ELISA, Flow, CyTOF-ready

3 Publications
ERK2 Antibody [Unconjugated]
AF1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class= ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, KO

3 Publications
Mxi1 Antibody [Unconjugated]
AF4185
Mxi1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human Mxi1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4185) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Human
Applications ICC

2 Publications
Coagulation Factor XIV/Protein C Antibody
AF4885

Sheep Polyclonal
Species Mouse
Applications WB, IP

2 Publications
Cyclin B1 Antibody [Unconjugated]
AF6000
Western blot shows lysates of U2OS human osteosarcoma cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IHC

12 Publications
PCNA Antibody (PC10)
NB500-106
Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     5 Reviews

46 Publications
APC Antibody - BSA Free
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate. Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

2 Publications
MCAT Antibody (2F2)
H00027349-M01
Western Blot: MCAT Antibody (2F2) [H00027349-M01] - MT monoclonal antibody (M01), clone 2F2 Analysis of MT expression in HeLa.Immunohistochemistry-Paraffin: MCAT Antibody (2F2) [H00027349-M01] - Analysis of monoclonal antibody to MT on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, IHC

KIF2C Antibody (1G2)
H00011004-M01
Western Blot: KIF2C Antibody (1G2) [H00011004-M01] - KIF2C monoclonal antibody (M01), clone 1G2 Analysis of KIF2C expression in Hela S3 NE.Immunocytochemistry/Immunofluorescence: KIF2C Antibody (1G2) [H00011004-M01] - Analysis of monoclonal antibody to KIF2C on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

4 Publications