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![Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/SGSM3-Antibody-Western-Blot-NBP2-13304-img0004.jpg "Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13304-img0005.jpg)
![Immunohistochemistry-Paraffin: SGSM3 Antibody [NBP2-13304] - Staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Immunohistochemistry-Paraffin-NBP2-13304-img0001.jpg)
![Western Blot: SGSM3 Antibody [NBP2-60726] - Detection of human and mouse SGSM3 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-SGSM3 antibody NBP2-60726 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Western-Blot-NBP2-60726-img0003.jpg "Western Blot: SGSM3 Antibody [NBP2-60726] - Detection of human and mouse SGSM3 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-SGSM3 antibody NBP2-60726 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: SGSM3 Antibody [NBP2-60726] - Detection of human and mouse SGSM3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SGSM3 antibody NBP2-60726 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Western-Blot-NBP2-60726-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SGSM3 Antibody [NBP2-60726] - Detection of human SGSM3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-SGSM3 antibody NBP2-60726 (lot NBP2-60726-2) used for IP at 20 ul/mg lysate. SGSM3 was also immunoprecipitated by a previous lot of this antibody (lot NBP2-60726-1) and another rabbit anti-SGSM3 antibody. For blotting immunoprecipitated SGSM3, NBP2-60726 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Immunoprecipitation-NBP2-60726-img0002.jpg)
![Western Blot: SGSM3 Antibody [NBP2-81814] - Analysis of SGSM3 in 293 cell lysate with SGSM3 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Western-Blot-NBP2-81814-img0001.jpg "Western Blot: SGSM3 Antibody [NBP2-81814] - Analysis of SGSM3 in 293 cell lysate with SGSM3 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-81814] - Immunofluorescence of SGSM3 in 293 cells with SGSM3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-81814-img0002.jpg)
![Western Blot: SGSM3 Antibody - BSA Free [NBP2-81814] - Western blot analysis of SGSM3 in 293 cell lysate with SGSM3 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81814_rabbit-polyclonal-sgsm3-antibody-2711202419333463.gif)
![Western Blot: SGSM3 Antibody [NBP1-45207] - Analysis of SGSM3 in A549 lysate (35ug protein in RIPA buffer) using this antibody at 2 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Western-Blot-NBP1-45207-img0002.jpg "Western Blot: SGSM3 Antibody [NBP1-45207] - Analysis of SGSM3 in A549 lysate (35ug protein in RIPA buffer) using this antibody at 2 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: SGSM3 Antibody [NBP2-88257] - Host: Rabbit. Target Name: SGSM3. Sample Tissue: HCT15 Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/SGSM3-Antibody-Western-Blot-NBP2-88257-img0001.jpg "Western Blot: SGSM3 Antibody [NBP2-88257] - Host: Rabbit. Target Name: SGSM3. Sample Tissue: HCT15 Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: SGSM3 Overexpression Lysate (Adult Normal) [NBL1-15914] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SGSM3.](http://images.novusbio.com/fullsize/SGSM3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15914-img0002.jpg "Western Blot: SGSM3 Overexpression Lysate (Adult Normal) [NBL1-15914] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SGSM3.")
![Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-97659] - Immunofluorescence staining of SGSM3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SGSM3 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/SGSM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97659-img0001.jpg "Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-97659] - Immunofluorescence staining of SGSM3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SGSM3 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.")