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![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Western-Blot-NBP1-85729-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85729-img0010.jpg)
![Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Western-Blot-NBP1-85729-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0011.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0012.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0013.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0014.jpg)
![Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Western-Blot-NBP2-37626-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis using CDK1 mouse mAb against Hela (1), Jurkat (2), K562 (3), A431 (4), MCF-7 (5), RAW264.7 (6), NIN/3T3 (7), PC-12 (8), and Cos7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of Eca109 cells using CDK1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunocytochemistry-Immunofluorescence-NBP2-37626-img0003.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of spinal cord tissue using CDC2/CDK1 antibody (2G9). Image from verified customer review.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-Paraffin-NBP2-37626-img0007.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Analysis of ovarian cancer tissues (left) and lung cancer tissues (right) using CDK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-Immunohistochemistry-NBP2-37626-img0004.jpg)
![ELISA: CDC2/CDK1 Antibody (2G9) [NBP2-37626] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-2G9-ELISA-NBP2-37626-img0001.jpg)
![Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).](http://images.novusbio.com/fullsize/Cdc2-[p-Tyr15]-Antibody-Western-Blot-NB500-142-img0001.jpg "Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).")
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0011.jpg "Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Immunohistochemistry-Paraffin-H00000983-M04-img0007.jpg)
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0008.jpg)
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0009.jpg)
![Western Blot: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Analysis of CDC2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-Western-Blot-H00000983-M04-img0010.jpg)
![ELISA: CDC2/CDK1 Antibody (8F1) [H00000983-M04] - Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-8F1-ELISA-H00000983-M04-img0006.jpg)
![Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-288202412442960.jpg "Western Blot: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Western blot analysis of CDC2/CDK1 on different lysates with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: Jurkat cell lysate Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: PC-12 cell lysate Lane 8: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32753) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32753) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-298202414161863.jpg)
![Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1 with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDC2/CDK1 antibody (NBP3-32753) at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202410334043.jpg)
![Flow Cytometry: CDC2/CDK1 [p Thr161] Antibody (PSH03-42) [NBP3-32753] - Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling CDC2/CDK1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32753, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32753_rabbit-cdc2-cdk1-p-thr161-mab-psh03-42-29202416372140.jpg)
![Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Western-Blot-NBP2-34331-img0003.jpg "Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) [NBP2-34331] - Analysis using the Azide and BSA Free version of NBP2-34331. Staining of human Tonsil stained with Cdk1 MAb (POH-1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Immunohistochemistry-Paraffin-NBP2-34331-img0001.jpg)
![Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) - Azide and BSA Free [NBP2-34711] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Azide-and-BSA-Free-Western-Blot-NBP2-34711-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) - Azide and BSA Free [NBP2-34711] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (POH-1; same as cdc2.1)")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (POH-1; same as cdc2.1) - Azide and BSA Free [NBP2-34711] - Formalin-paraffin human Tonsil stained with Cdk1 MAb (POH-1).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-POH-1;-same-as-cdc2-1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34711-img0001.jpg)
![Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.Lane 1: CDC2 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Western-Blot-H00000983-M01-img0011.jpg "Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody (M01), clone 1A4-1A9.Lane 1: CDC2 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of monoclonal antibody to CDC2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Immunocytochemistry-Immunofluorescence-H00000983-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Analysis of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 0.3 ug/ml.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Immunohistochemistry-Paraffin-H00000983-M01-img0008.jpg)
![Western Blot: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - CDC2 monoclonal antibody (M01), clone 1A4-1A9 Analysis of CDC2 expression in Hela.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-Western-Blot-H00000983-M01-img0010.jpg)
![ELISA: CDC2/CDK1 Antibody (1A4-1A9) [H00000983-M01] - Detection limit for recombinant GST tagged CDC2 is approximately 30ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-1A4-1A9-ELISA-H00000983-M01-img0012.jpg)
![Western Blot: CDC2/CDK1 Antibody (CDK1/873) [NBP2-44915] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (CDK1/873).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-CDK1-873-Western-Blot-NBP2-44915-img0002.jpg "Western Blot: CDC2/CDK1 Antibody (CDK1/873) [NBP2-44915] - Western Blot Analysis of human HeLa cell lysate using CDC2/CDK1 Antibody (CDK1/873).")
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody (CDK1/873) [NBP2-44915] - Human Tonsil stained with Cdk1 Monoclonal Antibody (CDK1/873).](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-CDK1-873-Immunohistochemistry-Paraffin-NBP2-44915-img0001.jpg)
![Western Blot: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161) antibody.](http://images.novusbio.com/fullsize/CDC2-CDK1-[p-Thr161]-Antibody-Western-Blot-NB100-81837-img0005.jpg "Western Blot: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Thr161) antibody.")
![Immunohistochemistry-Paraffin: CDC2/CDK1 [p Thr161] Antibody [NB100-81837] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81837 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/CDC2-CDK1-[p-Thr161]-Antibody-Immunohistochemistry-NB100-81837-img0004.jpg)
![Western Blot: CDK1/2 [p Thr 14] Antibody (SR1565) [NBP3-22648] - Western blot analysis using NBP3-22648 in HeLa cell lysate treated with hydroxyurea.](http://images.novusbio.com/fullsize/nbp3-22648_rabbit-cdk1-2-p-thr-14-mab-sr1565-6720239533718.jpg "Western Blot: CDK1/2 [p Thr 14] Antibody (SR1565) [NBP3-22648] - Western blot analysis using NBP3-22648 in HeLa cell lysate treated with hydroxyurea.")
![Immunohistochemistry: CDK1/2 [p Thr 14] Antibody (SR1565) [NBP3-22648] - Immunohistochemical analysis of paraffin-embedded rat spleen, using NBP3-22648.](http://images.novusbio.com/fullsize/nbp3-22648_rabbit-cdk1-2-p-thr-14-mab-sr1565-5720231754888.jpg)
![Western Blot: CDK1/2/3 [p Thr 14] Antibody (SR1566) [NBP3-22647] - Western blot analysis using NBP3-22647 in (1) A431 cell lysate; (2) Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-22647_rabbit-cdk1-2-3-p-thr-14-mab-sr1566-5720231721514.jpg "Western Blot: CDK1/2/3 [p Thr 14] Antibody (SR1566) [NBP3-22647] - Western blot analysis using NBP3-22647 in (1) A431 cell lysate; (2) Hela cell lysate.")