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Brain Segmentation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Brain Segmentation Pathway and Brain Infarction, Brain Diseases, Depressive Disorder, Cerebrovascular Disorders, Mild Cognitive Disorder. The study of the Brain Segmentation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Brain Segmentation Pathway has been researched in relation to Segmentation, Localization, Brain Development, Pathogenesis, Aging. The Brain Segmentation Pathway complements our catalog of research reagents including antibodies and ELISA kits against LAMC2, CSF2, MRF, DNER, FASTK.

Top Research Reagents

We have 1826 products for the study of the Brain Segmentation Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP3-15868
Western Blot: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Analysis of extracts of various cell lines, using Ceruloplasmin antibody (NBP3-15868) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunohistochemistry: Ceruloplasmin Antibody (6C3K9) [NBP3-15868] - Immunofluorescence analysis of mouse liver using Ceruloplasmin Rabbit mAb (NBP3-15868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-31323
Western Blot: FASTK Antibody [NBP1-31323] - A. 30 ug PC-12 whole cell lysate/extract 7.5 % SDS-PAGEFAST antibody dilution: 1:1000Immunocytochemistry/Immunofluorescence: FASTK Antibody [NBP1-31323] -  Analysis of methanol-fixed HeLa, using FAST antibody at 1:200 dilution.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-47974
Western Blot: PMEL17/SILV Antibody (10E5) [NBP1-47974] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PMEL17/SILV(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PMEL17/SILV.Immunocytochemistry/Immunofluorescence: PMEL17/SILV Antibody (10E5) [NBP1-47974] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PMEL17/SILV.

Mouse Monoclonal
Species Human, Rat, Canine
Applications WB, Flow, ICC/IF

     1 Review

NBP1-86893
Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Analysis in human skin and lymph node tissues. Corresponding DCT RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: TRP2 Antibody [NBP1-86893] - Staining of human cerebral cortex, lymph node, skin and testis using Anti-TRP2 antibody NBP1-86893 (A) shows similar protein distribution across tissues to independent antibody NBP1-86892 (B).

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

2 Publications
NBP1-87581
Immunohistochemistry-Paraffin: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Analysis in human small intestine and stomach tissues. Corresponding SI RNA-seq data are presented for the same tissues.Western Blot: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Analysis in human small intestine tissue.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP1-89284
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Rabbit Polyclonal
Species Human, Porcine
Applications WB, ICC/IF, IHC

     1 Review

5 Publications
NBP1-89946
Immunocytochemistry/Immunofluorescence: Dystonin Antibody [NBP1-89946] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.Immunohistochemistry-Paraffin: Dystonin Antibody [NBP1-89946] - Staining of human tonsil shows low positivity in non-germinal center cells as expected.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NB110-60531
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE was detected in immersion fixed paraffin-embedded sections of human liver using anti-human mouse monoclonal antibody (Catalog # NB110-60531) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.Western Blot: Apolipoprotein E/ApoE Antibody (WUE-4) [NB110-60531] - ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain

Mouse Monoclonal
Species Human, Mouse, Rat (Negative)
Applications WB, ELISA, Flow

     2 Reviews

36 Publications
NBP2-01743
Western Blot: Frataxin Antibody (1C12) [NBP2-01743] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Frataxin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Frataxin.Immunocytochemistry/Immunofluorescence: Frataxin Antibody (1C12) [NBP2-01743] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Frataxin.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

NBP2-02623
Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NBP2-20383
Western Blot: SLC17A5 Antibody [NBP2-20383] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: SLC17A5 Antibody [NBP2-20383] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: SLC17A5 protein stained by SLC17A5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

AF1356
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9-2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     4 Reviews

110 Publications
AF1513
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for <A class=

Goat Polyclonal
Species Mouse
Applications WB, Simple Western, Flow

     1 Review

7 Publications
AF2254
DNER was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=DNER does not activate, function with, or bind to Notch, but known Notch ligand DLL1 does.(A) Pooled luciferase results from 4 separate experiments (normalized to the mean of empty vector in each experiment). U2OS cells were transfected with ligand (DLL1, DNER, or EV), and separately a population of U2OS cells was transfected to express Notch, the control luciferase Renilla, and TP1, a promoter that expresses firefly luciferase when Notch is activated. The two populations were co-cultured 24 hours after transfection (trans configuration), and activity read after an additional 24–48 hours of incubation. (B) C2C12 cells (myoblasts) were incubated with differentiation media (2% horse serum) that either had pre-clustered DLL1-fc (1:1), pre-clustered DNER-fc (1:1), un-clustered DNER-fc, or fc only, all at a ratio of 1:150 in media. Cells were incubated for 72 hours, then fixed, and stained for the presence of myosin heavy chain (MHC) and nuclei. By measuring the percent of total nuclei that were inside of differentiated MHC positive myotubes, fusion indexes were calculated. (C) DNER (top left, green) transfected U2OS cells were not labeled by pre-clustered Notch-fc (top middle, red) but DLL1 (bottom left, green) transfected U2OS cells were labeled by pre-clustered Notch-fc (bottom middle, red). Merged images are shown at far right. Scale 10 μM. **** = p value <0.0001. *** = p value 0.002. ns = not significant. DLL1 = Delta-like 1, a known Notch Ligand, DNER = Delta/Notch-like epidermal growth factor (EGF) related receptor, GSI =  gamma -secretase inhibitor, fc only = rabbit anti-human-fc. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0161157), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Mouse
Applications WB, IHC

12 Publications
NB100-2564
Western Blot: FACA/FANCA Antibody [NB100-2564] - Detection of FANC A in transfected COS1 cell lysate.

Rabbit Polyclonal
Species Human
Applications WB

1 Publication
7954-GM/CF
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED<sub>50</sub> for this effect is 6-30 pg/mL.


Species Human
Applications BA

3 Publications
NBP2-42388
Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

4 Publications
NBP2-92630
Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-92630] - Analysis of extracts of various cell lines, using Adenine Nucleotide Translocator 2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.Immunocytochemistry/Immunofluorescence: Adenine Nucleotide Translocator 2 Antibody [NBP2-92630] - Analysis of HeLa cells using Adenine Nucleotide Translocator 2 . Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

H00008518-M03
Western Blot: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of IKBKAP expression in HeLa (Cat # L013V1).Immunohistochemistry-Paraffin: IKBKAP Antibody (6G9) [H00008518-M03] - Analysis of monoclonal antibody to IKBKAP on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

2 Publications