![Western Blot: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Western Blot analysis of Human Cell lysates showing detection of Kv7.1 protein using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-1-Antibody-S37A-10-Western-Blot-NBP2-12897-img0010.jpg "Western Blot: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Western Blot analysis of Human Cell lysates showing detection of Kv7.1 protein using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
| Reactivity | Hu, Mu, Rt, HaSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, MA |
| Clone | S37A/10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein amino acids 2-101 of human KCNQ1 |
| Localization | Cell Membrane, Cytoplasmic Vesicle Membrane |
| Specificity | Detects approx 75 kDa. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KCNQ1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of KCNQ1 Antibody was sufficient for detection of KCNQ1 in 10 ug of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Kv7.1 Antibody (NBP2-12897)Find related products by research area.
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| Gene Symbol | KCNQ1 |
![Immunocytochemistry/Immunofluorescence: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kv7.1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Kv7-1-Antibody-S37A-10-Immunocytochemistry-Immunofluorescence-NBP2-12897-img0013.jpg "Immunocytochemistry/Immunofluorescence: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kv7.1 Antibody (D) Composite.")
![Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunohistochemistry analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/Kv7-1-Antibody-S37A-10-Immunohistochemistry-NBP2-12897-img0012.jpg "Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunohistochemistry analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.")
![Western Blot: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Analysis of hamster T-CHO cell lysate showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A/10. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-1-Antibody-S37A-10-Western-Blot-NBP2-12897-img0008.jpg "Western Blot: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Analysis of hamster T-CHO cell lysate showing detection of KCNQ1 protein using Mouse Anti-KCNQ1 Monoclonal Antibody, Clone S37A/10. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-1-Antibody-S37A-10-Immunohistochemistry-NBP2-12897-img0009.jpg "Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.")
![Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunohistochemistry analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-1-Antibody-S37A-10-Immunohistochemistry-NBP2-12897-img0011.jpg "Immunohistochemistry: Kv7.1 Antibody (S37A/10) [NBP2-12897] - Immunohistochemistry analysis using Mouse Anti-Kv7.1 Monoclonal Antibody, Clone N37A/10 (NBP2-12897). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv7.1 Monoclonal Antibody (NBP2-12897) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")