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![Western Blot: WDR77 Antibody [NBP1-82778] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/WDR77-Antibody-Western-Blot-NBP1-82778-img0006.jpg "Western Blot: WDR77 Antibody [NBP1-82778] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: WDR77 Antibody [NBP1-82778] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82778-img0008.jpg)
![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human rectum shows weak to moderate cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP1-82778-img0013.jpg)
![Western Blot: WDR77 Antibody [NBP1-82778] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/WDR77-Antibody-Western-Blot-NBP1-82778-img0005.jpg)
![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human fallopian tube shows cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP1-82778-img0009.jpg)
![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-NBP1-82778-img0010.jpg)
![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP1-82778] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP1-82778-img0012.jpg)
![Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - WDR77 monoclonal antibody (M01), clone 3F10 Analysis of WDR77 expression in HeLa.](http://images.novusbio.com/fullsize/WDR77-Antibody-3F10-Western-Blot-H00079084-M01-img0006.jpg "Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - WDR77 monoclonal antibody (M01), clone 3F10 Analysis of WDR77 expression in HeLa.")
![Immunohistochemistry-Paraffin: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/WDR77-Antibody-3F10-Immunohistochemistry-Paraffin-H00079084-M01-img0004.jpg)
![Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.Lane 1: WDR77 transfected lysate(36.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/WDR77-Antibody-3F10-Western-Blot-H00079084-M01-img0007.jpg)
![Sandwich ELISA: WDR77 Antibody (3F10) [H00079084-M01] - Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/WDR77-Antibody-3F10-Sandwich-ELISA-H00079084-M01-img0005.jpg)
![Western Blot: WDR77 Antibody [NB100-77305] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WDR77/MEP50 antibody sed for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WDR77/MEP50 was also immunoprecipitated by rabbit anti-WDR77/MEP50 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).](http://images.novusbio.com/fullsize/WDR77-Antibody-Western-Blot-NB100-77305-img0008.jpg "Western Blot: WDR77 Antibody [NB100-77305] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WDR77/MEP50 antibody sed for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WDR77/MEP50 was also immunoprecipitated by rabbit anti-WDR77/MEP50 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).")
![Immunohistochemistry: WDR77 Antibody [NB100-77305] - Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti- WDR77/MEP50 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-NB100-77305-img0006.jpg)
![Western Blot: WDR77 Antibody [NBP2-20901] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/WDR77-Antibody-Western-Blot-NBP2-20901-img0001.jpg "Western Blot: WDR77 Antibody [NBP2-20901] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: WDR77 Antibody [NBP2-20901] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20901-img0003.jpg)
![Immunohistochemistry-Paraffin: WDR77 Antibody [NBP2-20901] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-Paraffin-NBP2-20901-img0002.jpg)
![Western Blot: WDR77 Antibody (PSH0-07) [NBP3-33101] - Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (NBP3-33101) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: 293 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33101) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33101_rabbit-wdr77-mab-psh0-07-288202414191530.jpg "Western Blot: WDR77 Antibody (PSH0-07) [NBP3-33101] - Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (NBP3-33101) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: 293 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33101) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: WDR77 Antibody (PSH0-07) [NBP3-33101] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-WDR77 antibody (NBP3-33101) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33101) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33101_rabbit-wdr77-mab-psh0-07-29820241492671.jpg)
![Flow Cytometry: WDR77 Antibody (PSH0-07) [NBP3-33101] - Flow cytometric analysis of Hela cells labeling WDR77. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33101, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33101_rabbit-wdr77-mab-psh0-07-29202416453226.jpg)
![Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] - Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-27910_mouse-wdr77-mab-8b2f7-2711202419293883.jpg "Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] - Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: WDR77 Antibody (8B2F7) [NBP3-27910] - Western blot analysis of extracts of various cell lines, using WDR77 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-27910_mouse-wdr77-mab-8b2f7-271120241918625.jpg)
![Immunohistochemistry: WDR77 Antibody [NBP1-82780] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-NBP1-82780-img0005.jpg "Immunohistochemistry: WDR77 Antibody [NBP1-82780] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.")
![Immunohistochemistry: WDR77 Antibody [NBP1-82779] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/WDR77-Antibody-Immunohistochemistry-NBP1-82779-img0007.jpg "Immunohistochemistry: WDR77 Antibody [NBP1-82779] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.")
![Western Blot: WDR77 Overexpression Lysate (Adult Normal) [NBL1-17831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WDR77.](http://images.novusbio.com/fullsize/WDR77-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17831-img0002.jpg "Western Blot: WDR77 Overexpression Lysate (Adult Normal) [NBL1-17831] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WDR77.")