| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-119SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6687-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | FAB66871A | |||
| Phycoerythrin | FAB66871P-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB66871A | |||
| Carrier Free | MAB66871-SP | |||
| Carrier Free | FAB66871P-100 | |||
![Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0009.jpg "Western Blot: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate.")
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Immunostain of NK1R in rat enteric glial cells. Image from verified customer review.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-119-img0004.jpg)
![Immunohistochemistry: NK1R Antibody [NB300-119] - Neurokinin 1 Receptor Antibody [NB300-119] - Staining of human brain, basal nucleus of Meynert, neurons.](http://images.novusbio.com/fullsize/NK1R-Antibody-Immunohistochemistry-NB300-119-img0006.jpg)
![Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-NK1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0010.jpg)
![Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NK1R-Antibody-Western-Blot-NB300-119-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Overnight incubation at 4C with NK1R antibody diluted 1:100 in PBS/BSA 1%. Red: Phalloidin, Blue: DAPI, Green: NK1R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb300-119_rabbit-polyclonal-nk1r-antibody-immunocytochemistry-immunofluorescence-1372023145313..jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - TACR1 & its downstream targets SRC & p-SRC are expressed in neuroblastoma cell lines(A) Endogenous TACR1, SRC & p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. (B) Quantitative RT-PCR of TACR1 mRNA in a panel of neuroblastoma cell lines (n = 3, error bars indicate standard deviation). (C–E) Quantification of SRC (C), p-SRC (D) & TACR1 (E) protein expression using densitometry analysis of western immunoblots (n = 3, error bars indicate standard deviation). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202415392524.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416205147.jpg)
![Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta 3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta 3 subunit from individual 30-day-old mice revealed reduced amounts of beta 3 subunit protein in striatum (B) from Cre positive ( beta 3f/fDrd2) animals compared to Cre negative ( beta 3f/+ & beta 3f/f) control animals. The amount of beta 3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta -actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta 3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-119_rabbit-polyclonal-nk1r-antibody-310202416212385.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0007.jpg "Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.")
![Immunohistochemistry: Neurokinin 1 Receptor Antibody [NB300-101] - Immunohistochemical analysis of Neurokinin 1 Receptor in mouse brain (selected cells, axon & dendrites) using NB300-101.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunohistochemistry-NB300-101-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (NB 300-101) in a lamina III neuron in the spinal cord of a rat.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [NB300-101] - IF analysis of Neurokinin 1 Receptor in rat brain tissue. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NB300-101-img0010.jpg)
![Immunohistochemistry: NK1R Antibody Pack [NB100-912] - Neurokinin 1 Receptor Antibody Pack [NB100-912] - Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (NB 300-101) in a lamina III neuron in the spinal cord of a rat.](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Pack-Immunohistochemistry-NB100-912-img0004.jpg "Immunohistochemistry: NK1R Antibody Pack [NB100-912] - Neurokinin 1 Receptor Antibody Pack [NB100-912] - Confocal image of SPR reactivity using polyclonal anti-NK-1 Receptor (NB 300-101) in a lamina III neuron in the spinal cord of a rat.")
![Western Blot: NK1R Antibody Pack [NB100-912] - Analysis of Neurokinin 1 Receptor in 1: human brain lysate, 2: rat brain lysate and 3: monkey brain lysate. Neurokinin 1 Receptor Antibody [NB300-119]](http://images.novusbio.com/fullsize/NK1R-Antibody-Pack-Western-Blot-NB100-912-img0007.jpg)
![Immunohistochemistry: NK1R Antibody Pack [NB100-912] - Neurokinin 1 Receptor Antibody Pack [NB100-912] - Brain, Basal Nucleus of Meynert, Neurons 40X. Neurokinin 1 Receptor Antibody [NB300-119]](http://images.novusbio.com/fullsize/Neurokinin-1-Receptor-Antibody-Pack-Immunohistochemistry-NB100-912-img0003.jpg)
![Western Blot: NK1R Antibody (JE39-75) [NBP3-32653] - Western blot analysis of NK1R on different lysates with Rabbit anti-NK1R antibody (NBP3-32653) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: NCCIT cell lysate Lane 5: F9 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32653) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32653_rabbit-nk1r-mab-je39-75-278202415212471.jpg "Western Blot: NK1R Antibody (JE39-75) [NBP3-32653] - Western blot analysis of NK1R on different lysates with Rabbit anti-NK1R antibody (NBP3-32653) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: NCCIT cell lysate Lane 5: F9 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32653) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NK1R Antibody (JE39-75) [NBP3-32653] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-NK1R antibody (NBP3-32653) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32653) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32653_rabbit-nk1r-mab-je39-75-298202413585593.jpg)
![Immunocytochemistry/ Immunofluorescence: NK1R Antibody (JE39-75) [NBP3-32653] - Immunocytochemistry analysis of Neuro-2a cells labeling NK1R with Rabbit anti-NK1R antibody (NBP3-32653) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NK1R antibody (NBP3-32653) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32653_rabbit-nk1r-mab-je39-75-29202410234275.jpg)
![Flow Cytometry: NK1R Antibody (JE39-75) [NBP3-32653] - Flow cytometric analysis of Neuro-2a cells labeling NK1R. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32653, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32653_rabbit-nk1r-mab-je39-75-29202416453284.jpg)
![Western Blot: NK1R Antibody (9T1W2) [NBP3-16461] - Western blot analysis of extracts of various cell lines, using NK1R (NK1R) Rabbit mAb (NBP3-16461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/NK1R-Antibody-9T1W2-Western-Blot-NBP3-16461-img0001.jpg "Western Blot: NK1R Antibody (9T1W2) [NBP3-16461] - Western blot analysis of extracts of various cell lines, using NK1R (NK1R) Rabbit mAb (NBP3-16461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: NK1R Antibody (9T1W2) [NBP3-16461] - Western blot analysis of extracts of various cell lines, using NK1R (NK1R) Rabbit mAb (NBP3-16461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/NK1R-Antibody-9T1W2-Western-Blot-NBP3-16461-img0002.jpg)
![Western Blot: NK1R Antibody (1E5) [NBP3-15240] - Positive WB detected in: THP-1 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Ntera-2 whole cell lysate, Mouse Brain whole cell lysate. All lanes: NK1R antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 36 KDa. Observed band size: 50 KDa.](http://images.novusbio.com/fullsize/NK1R-Antibody-1E5-Western-Blot-NBP3-15240-img0001.jpg "Western Blot: NK1R Antibody (1E5) [NBP3-15240] - Positive WB detected in: THP-1 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Ntera-2 whole cell lysate, Mouse Brain whole cell lysate. All lanes: NK1R antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 36 KDa. Observed band size: 50 KDa.")