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![Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/StAR-Antibody-Western-Blot-NBP1-33485-img0011.jpg "Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: StAR Antibody [NBP1-33485] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by StAR antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33485-img0005.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Human hepatoma. StAR antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-33485-img0004.jpg)
![Western Blot: StAR Antibody [NBP1-33485] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/StAR-Antibody-Western-Blot-NBP1-33485-img0007.jpg)
![Western Blot: StAR Antibody [NBP1-33485] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody (NBP1-33485) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33485_rabbit-polyclonal-star-antibody-91020242011464.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: StAR Antibody [NBP1-33485] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33485_rabbit-polyclonal-star-antibody-1610202419414573.jpg)
![Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249362728.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249383148.jpg)
![Western Blot: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (NBP3-25696) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249415966.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249425487.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249413335.jpg)
![Immunocytochemistry/Immunofluorescence: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249374934.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249415968.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (HL1360) - Azide and BSA Free [NBP3-25696] - StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. StAR stained by StAR antibody [HL1360] (NBP3-25696) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25696_rabbit-star-mab-hl1360-azide-and-bsa-free-2120249413391.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human lymph node.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0010.jpg "Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human lymph node.")
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0004.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining in human adrenal gland and pancreas tissues using anti-STAR antibody. Corresponding STAR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human adrenal gland, kidney, lymph node and testis using Anti-STAR antibody NBP1-86992 (A) shows similar protein distribution across tissues to independent antibody NBP1-86993 (B).](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0007.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0008.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human testis.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0009.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86992] - Staining of human kidney.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86992-img0011.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining of human lymph node.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86993-img0014.jpg "Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining of human lymph node.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining in human adrenal gland and skeletal muscle tissues.. Corresponding STAR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86993-img0007.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining of human testis shows strong positivity in mitochondria in Leydig cells.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86993-img0008.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86993-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining of human adrenal gland, kidney, lymph node and testis using Anti-STAR antibody NBP1-86993 (A) shows similar protein distribution across tissues to independent antibody NBP1-86992 (B).](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86993-img0011.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining of human kidney.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86993-img0012.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-86993] - Staining of human adrenal gland shows strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-86993-img0013.jpg)
![Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/StAR-Antibody-5F9-Western-Blot-H00006770-M01-img0006.jpg "Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR expression in transfected 293T cell line by STAR monoclonal antibody (M01), clone 5F9.Lane 1: STAR transfected lysate(31.9 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: StAR Antibody (5F9) [H00006770-M01] - Analysis of STAR over-expressed 293 cell line, cotransfected with STAR Validated Chimera RNAi ( Cat # H00006770-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STAR monoclonal antibody (M01), clone 5F9 (Cat # H00006770-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/StAR-Antibody-5F9-Western-Blot-H00006770-M01-img0004.jpg)
![Sandwich ELISA: StAR Antibody (5F9) [H00006770-M01] - Detection limit for recombinant GST tagged STAR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/StAR-Antibody-5F9-Sandwich-ELISA-H00006770-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2154) [NBP2-75741] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2154).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2154-Immunohistochemistry-Paraffin-NBP2-75741-img0006.jpg "Immunohistochemistry-Paraffin: StAR Antibody (STAR/2154) [NBP2-75741] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2154).")
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2154) [NBP2-75741] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with StAR Mouse Monoclonal Antibody (STAR/2154).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2154-Immunohistochemistry-Paraffin-NBP2-75741-img0001.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2154) [NBP2-75741] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2154).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2154-Immunohistochemistry-Paraffin-NBP2-75741-img0002.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2154) [NBP2-75741] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2154).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2154-Immunohistochemistry-Paraffin-NBP2-75741-img0003.jpg)
![Protein Array: StAR Antibody (STAR/2154) [NBP2-75741] - Analysis of Protein Array containing more than 19,000 full-length human proteins using StAR Antibody (STAR/2154). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2154-Protein-Array-NBP2-75741-img0005.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2140) [NBP2-75739] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2140).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2140-Immunohistochemistry-Paraffin-NBP2-75739-img0002.jpg "Immunohistochemistry-Paraffin: StAR Antibody (STAR/2140) [NBP2-75739] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with StAR Mouse Monoclonal Antibody (STAR/2140).")
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2140) [NBP2-75739] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with StAR Mouse Monoclonal Antibody (STAR/2140).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2140-Immunohistochemistry-Paraffin-NBP2-75739-img0001.jpg)
![Protein Array: StAR Antibody (STAR/2140) [NBP2-75739] - Analysis of Protein Array containing more than 19,000 full-length human proteins using StAR Antibody (STAR/2140). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2140-Protein-Array-NBP2-75739-img0004.jpg)
![Western Blot: StAR Antibody (JE32-23) [NBP3-32979] - Western blot analysis of StAR on K-562 cell lysates with Rabbit anti-StAR antibody (NBP3-32979) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32979) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32979_rabbit-star-mab-je32-23-28820241249348.jpg "Western Blot: StAR Antibody (JE32-23) [NBP3-32979] - Western blot analysis of StAR on K-562 cell lysates with Rabbit anti-StAR antibody (NBP3-32979) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32979) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: StAR Antibody (JE32-23) [NBP3-32979] - Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-StAR antibody (NBP3-32979) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32979) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32979_rabbit-star-mab-je32-23-29820241492622.jpg)
![Western Blot: StAR Antibody (2B5) [H00006770-M10] - Detection against Immunogen (36.63 KDa) .](http://images.novusbio.com/fullsize/StAR-Antibody-2B5-Western-Blot-H00006770-M10-img0001.jpg "Western Blot: StAR Antibody (2B5) [H00006770-M10] - Detection against Immunogen (36.63 KDa) .")
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2077) [NBP3-07308] - Formalin-fixed, paraffin-embedded human Stomach stained with StAR Mouse Monoclonal Antibody (STAR/2077).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2077-Immunohistochemistry-Paraffin-NBP3-07308-img0002.jpg "Immunohistochemistry-Paraffin: StAR Antibody (STAR/2077) [NBP3-07308] - Formalin-fixed, paraffin-embedded human Stomach stained with StAR Mouse Monoclonal Antibody (STAR/2077).")
![Immunohistochemistry-Paraffin: StAR Antibody (STAR/2077) [NBP3-07308] - Formalin-fixed, paraffin-embedded human Testis stained with StAR Mouse Monoclonal Antibody (STAR/2077).](http://images.novusbio.com/fullsize/StAR-Antibody-STAR-2077-Immunohistochemistry-Paraffin-NBP3-07308-img0001.jpg)
![Western Blot: StAR Antibody (S04-5G9) [NBP3-19536] - Western blot detection of StAR in K562, 3T3, Hela cell lysates using NBP3-19536 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.](http://images.novusbio.com/fullsize/StAR-Antibody-S04-5G9-Western-Blot-NBP3-19536-img0001.jpg "Western Blot: StAR Antibody (S04-5G9) [NBP3-19536] - Western blot detection of StAR in K562, 3T3, Hela cell lysates using NBP3-19536 (1:1000 diluted). Predicted band size: 32kDa. Observed band size: 32kDa.")
![Western Blot: StAR Overexpression Lysate (Adult Normal) [NBP2-09031] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for StAR.](http://images.novusbio.com/fullsize/StAR-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09031-img0001.jpg "Western Blot: StAR Overexpression Lysate (Adult Normal) [NBP2-09031] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for StAR.")