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![Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Western-Blot-NBP2-03285-img0020.jpg "Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-NBP2-03285-img0019.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human tonsil using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0018.jpg)
![Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Jurkat cells, using anti-SAMHD1 antibody,(Red), compared to a nonspecific negative control antibody,(Blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Flow-Cytometry-NBP2-03285-img0022.jpg)
![Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Western-Blot-NBP2-03285-img0012.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0014.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Carcinoma of Human liver tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0015.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human lymphoma tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0016.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human pancreas tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0017.jpg)
![Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Hela cells, using anti-SAMHD1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Flow-Cytometry-NBP2-03285-img0011.jpg)
![Knockout Validated: SAMHD1 Antibody [NBP1-31432] - Wild-type (WT) and SAMHD1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAMHD1 antibody [N2C2], Internal. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Western-Blot-NBP1-31432-img0006.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: SAMHD1 Antibody [NBP1-31432] - Wild-type (WT) and SAMHD1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAMHD1 antibody [N2C2], Internal. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: SAMHD1 Antibody [NBP1-31432] - Sample (30 ug of whole cell lysate) A: THP-1 7.5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Western-Blot-NBP1-31432-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody [NBP1-31432] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31432-img0003.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP1-31432] - SkHep1 xenograft. SAMHD1 antibody [N2C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-31432-img0005.jpg)
![Western Blot: SAMHD1 Antibody [NBP2-27410] - Western blot analysis of SAMHD1 using SAMHD1 antibody. Human heart tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 1 ug/ml of SAMHD1 antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Western-Blot-NBP2-27410-img0003.jpg "Western Blot: SAMHD1 Antibody [NBP2-27410] - Western blot analysis of SAMHD1 using SAMHD1 antibody. Human heart tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 1 ug/ml of SAMHD1 antibody.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-27410] - Analysis of SAMHD1 using SAMHD1 antibody. Human kidney probed with SAMHD1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-27410-img0002.jpg)
![Western Blot: SAMHD1 Antibody [NBP2-27097] - Western blot analysis of SAMHD1 using SAMHD1 antibody. Human heart tissue lysate in the 1) pre immune sera and 2) absence of immunizing peptide and 3) spleen lysate probed with 2 ug/ml of SAMHD1 antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Western-Blot-NBP2-27097-img0003.jpg "Western Blot: SAMHD1 Antibody [NBP2-27097] - Western blot analysis of SAMHD1 using SAMHD1 antibody. Human heart tissue lysate in the 1) pre immune sera and 2) absence of immunizing peptide and 3) spleen lysate probed with 2 ug/ml of SAMHD1 antibody.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-27097] - Staining of SAMHD1 in human kidney tissue using SAMHD1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-27097-img0002.jpg)
![Western Blot: SAMHD1 Antibody [NBP2-47292] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human Analysis of liver tissue. Lane 6: Human tonsil tissue.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Western-Blot-NBP2-47292-img0003.jpg "Western Blot: SAMHD1 Antibody [NBP2-47292] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human Analysis of liver tissue. Lane 6: Human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody [NBP2-47292] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47292-img0004.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-47292] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-47292-img0006.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-47292] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-47292-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-47292] - Staining in human lymph node and skeletal muscle tissues using anti-SAMHD1 antibody. Corresponding SAMHD1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-47292-img0007.jpg)
![Western Blot: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Western-Blot-NBP2-12776-img0007.jpg "Western Blot: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (T; 50 ug), and Jurkat (J; 50 ug) cells. Antibody: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-NBP2-12776-img0003.jpg)
![Immunohistochemistry-Paraffin: SAMHD1 Antibody [NBP2-12776] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAMHD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-12776-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: SAMHD1 Antibody [NBP2-12776] - Detection of human SAMHD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAMHD1 antibody NBP2-12776 (lot NBP2-12776-1) used for IP at 6 ug/mg lysate. SAMHD1 was also immunoprecipitated by another rabbit anti-SAMHD1 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated SAMHD1, NBP2-12776 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-Immunoprecipitation-NBP2-12776-img0006.jpg)
![Western Blot: SAMHD1 Antibody (4C7B3) [NBP3-16448] - Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (NBP3-16448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-4C7B3-Western-Blot-NBP3-16448-img0002.jpg "Western Blot: SAMHD1 Antibody (4C7B3) [NBP3-16448] - Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (NBP3-16448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (4C7B3) [NBP3-16448] - Immunohistochemistry of paraffin-embedded human appendix using SAMHD1 Rabbit mAb (NBP3-16448) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-4C7B3-Immunohistochemistry-Paraffin-NBP3-16448-img0001.jpg)
![Western Blot: SAMHD1 Overexpression Lysate (Adult Normal) [NBL1-15682] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SAMHD1.](http://images.novusbio.com/fullsize/SAMHD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15682-img0002.jpg "Western Blot: SAMHD1 Overexpression Lysate (Adult Normal) [NBL1-15682] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SAMHD1.")