![Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Western-Blot-NBP2-03285-img0020.jpg "Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SAMHD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publications using NBP2-03285 | Applications | Species |
|---|---|---|
| Deutschmann J, Schneider A, Gruska I et al. A viral kinase counteracts in vivo restriction of murine cytomegalovirus by SAMHD1 Nat Microbiol 2019-09-23 [PMID: 31548683] (WB, Mouse) | WB | Mouse |
| Businger R, Deutschmann J, Gruska I et al. Human cytomegalovirus overcomes SAMHD1 restriction in macrophages via pUL97 Nat Microbiol 2019-09-23 [PMID: 31548682] (WB, Human) | WB | Human |
| Kim YC, Kim KK, Yoon J et al. SAMHD1 Posttranscriptionally Controls the Expression of Foxp3 and Helios in Human T Regulatory Cells J. Immunol. 2018-08-13 [PMID: 30104243] (WB, Human) | WB | Human |
| Deutschmann J Regulatory Mechanism of the Innate Restriction Factor SAMHD1 in Herpesviral Infections Thesis 2020-01-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for SAMHD1 Antibody (NBP2-03285)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-NBP2-03285-img0019.jpg "Immunocytochemistry/Immunofluorescence: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human tonsil using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0018.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human tonsil using anti-SAMHD1 mouse monoclonal antibody.")
![Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Jurkat cells, using anti-SAMHD1 antibody,(Red), compared to a nonspecific negative control antibody,(Blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Flow-Cytometry-NBP2-03285-img0022.jpg "Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Jurkat cells, using anti-SAMHD1 antibody,(Red), compared to a nonspecific negative control antibody,(Blue).")
![Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Western-Blot-NBP2-03285-img0012.jpg "Western Blot: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0014.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Carcinoma of Human liver tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0015.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Carcinoma of Human liver tissue using anti-SAMHD1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human lymphoma tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0016.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human lymphoma tissue using anti-SAMHD1 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human pancreas tissue using anti-SAMHD1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Immunohistochemistry-Paraffin-NBP2-03285-img0017.jpg "Immunohistochemistry-Paraffin: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Staining of Human pancreas tissue using anti-SAMHD1 mouse monoclonal antibody.")
![Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Hela cells, using anti-SAMHD1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SAMHD1-Antibody-3F5-Flow-Cytometry-NBP2-03285-img0011.jpg "Flow Cytometry: SAMHD1 Antibody (OTI3F5) [NBP2-03285] - Analysis of Hela cells, using anti-SAMHD1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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