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![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody (M03), clone 1H4. Lane 1: RAF1 transfected lysatE (73.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0007.jpg "Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody (M03), clone 1H4. Lane 1: RAF1 transfected lysatE (73.1 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi or non-transfected control. Blot probed with H00005894-M03. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0002.jpg)
![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in HeLa.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0005.jpg)
![Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in rat brain.](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Western-Blot-H00005894-M03-img0006.jpg)
![Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between BAD and RAF1. HeLa cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-RAF1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction compl](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Proximity-Ligation-Assay-H00005894-M03-img0003.jpg)
![Proximity Ligation Assay: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of protein-protein interactions between PDGFRB and RAF1. Huh7 cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-RAF1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction](http://images.novusbio.com/fullsize/Raf-1-Antibody-1H4-Proximity-Ligation-Assay-H00005894-M03-img0004.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0003.jpg "Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.")
![Immunohistochemistry: Raf-1 Antibody (4G4) [NBP2-37513] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Immunohistochemistry-NBP2-37513-img0005.jpg)
![Flow Cytometry: Raf-1 Antibody (4G4) [NBP2-37513] - Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Flow-Cytometry-NBP2-37513-img0004.jpg)
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0002.jpg)
![ELISA: Raf-1 Antibody (4G4) [NBP2-37513] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-ELISA-NBP2-37513-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85462-img0006.jpg "Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.")
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human testis shows weak positivity in nuclear speckles in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0011.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cerebellum shows moderate positivity in nuclear speckles in Purkinje cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0008.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cervix shows moderate positivity in nuclear speckles in squamous epithelial cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0009.jpg)
![Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human rectum shows moderate positivity in nuclear speckles in glandular cells.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85462-img0010.jpg)
![Western Blot: Raf-1 [p Ser 259] Antibody (SR2181) [NBP3-21573] - Western blot analysis using NBP3-21573 in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21573_rabbit-raf-1-p-ser-259-mab-sr2181-57202315584846.jpg "Western Blot: Raf-1 [p Ser 259] Antibody (SR2181) [NBP3-21573] - Western blot analysis using NBP3-21573 in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.")
![Western Blot: Raf-1 Antibody (S05-9B1) [NBP3-14880] - Western blot detection of Raf-1 in C6, 3T3, Hela cell lysates using Raf-1 Rabbit mAb (1:1000 diluted). Predicted band size: 73kDa. Observed band size: 73kDa.](http://images.novusbio.com/fullsize/Raf-1-Antibody-S05-9B1-Western-Blot-NBP3-14880-img0001.jpg "Western Blot: Raf-1 Antibody (S05-9B1) [NBP3-14880] - Western blot detection of Raf-1 in C6, 3T3, Hela cell lysates using Raf-1 Rabbit mAb (1:1000 diluted). Predicted band size: 73kDa. Observed band size: 73kDa.")
![Immunocytochemistry/Immunofluorescence: Raf-1 Antibody (S05-9B1) [NBP3-14880] - Immunocytochemistry of Raf-1 (green) in MCF-7 using Raf-1 antibody at dilution 1/20, and DAPI (blue)](http://images.novusbio.com/fullsize/Raf-1-Antibody-S05-9B1-Immunocytochemistry-Immunofluorescence-NBP3-14880-img0002.jpg)
![Western Blot: Raf-1 Antibody [H00005894-B01P] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 polyclonal antibody. Lane 1: RAF1 transfected lysate(71.28 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-Western-Blot-H00005894-B01P-img0004.jpg "Western Blot: Raf-1 Antibody [H00005894-B01P] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 polyclonal antibody. Lane 1: RAF1 transfected lysate(71.28 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [H00005894-B01P] - Analysis of purified antibody to RAF1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/Raf-1-Antibody-Immunocytochemistry-Immunofluorescence-H00005894-B01P-img0003.jpg)
![Western Blot: Raf-1 Antibody (M208) [NBP3-23358] - Western blot of human A431 (lane 1) and Jurkat (lane 2) cells probed with mouse monoclonal anti-Raf-1 (N-terminal) antibody at 1:500.](http://images.novusbio.com/fullsize/nbp3-23358_mouse-raf-1-mab-m208-19920231856927.jpg "Western Blot: Raf-1 Antibody (M208) [NBP3-23358] - Western blot of human A431 (lane 1) and Jurkat (lane 2) cells probed with mouse monoclonal anti-Raf-1 (N-terminal) antibody at 1:500.")
![Western Blot: Raf-1 Antibody [NBP3-23356] - Western blot of GST fusion protein containing human Raf-1. The blot was probed with polyclonal anti-Raf-1 (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of Raf-1 (C-terminus) blocking peptide (lane 2), Raf-1 (Ser-471) peptide (lane 3), or unrelated peptide (lane 4).](http://images.novusbio.com/fullsize/nbp3-23356_rabbit-raf-1-pab-189202317355212.jpg "Western Blot: Raf-1 Antibody [NBP3-23356] - Western blot of GST fusion protein containing human Raf-1. The blot was probed with polyclonal anti-Raf-1 (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of Raf-1 (C-terminus) blocking peptide (lane 2), Raf-1 (Ser-471) peptide (lane 3), or unrelated peptide (lane 4).")
![Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] - Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-Raf-1 (N-terminal region) (lanes 1 & 2) or anti-Raf-1 (Ser-471) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23359_rabbit-raf-1-p-ser471-pab-19920231915416.jpg "Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] - Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-Raf-1 (N-terminal region) (lanes 1 & 2) or anti-Raf-1 (Ser-471) (lanes 3 & 4).")
![Western Blot: Raf-1 [p Ser 621] Antibody (SR2183) [NBP3-21572] - Western blot analysis using NBP3-21572 in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21572_rabbit-raf-1-p-ser-621-mab-sr2183-57202316582174.jpg "Western Blot: Raf-1 [p Ser 621] Antibody (SR2183) [NBP3-21572] - Western blot analysis using NBP3-21572 in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate.")
![Western Blot: Raf-1 [p Ser 43] Antibody (SR2182) [NBP3-21574] - Western blot analysis using NBP3-21574 in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21574_rabbit-raf-1-p-ser-43-mab-sr2182-5720231531212.jpg "Western Blot: Raf-1 [p Ser 43] Antibody (SR2182) [NBP3-21574] - Western blot analysis using NBP3-21574 in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate.")