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![Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NB300-137-img0004.jpg "Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Analysis of the widely used rat pheochromocytoma PC12 cell line stained with rabbit polyclonal Periphren antibody, dilution 1:2000 (Green). Hoechst staining of nuclear DNA (Blue). Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell forming a perinuclear cap, with some filaments visible in the cytoplasm.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0003.jpg)
![Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.](http://images.novusbio.com/fullsize/p_4356adfdef9d7.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-137 antibody to peripherin (green) and chicken polyclonal antibody to phosphorylated NF-H (red channel). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. The blue channel reveals the localization of DNA.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0001.jpg)
![Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NBP1-05423-img0003.jpg "Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - Analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, NBP1-05423, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. NBP1-05423 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0005.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (red) in nerve bundles in the human larynx. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - shows rat mixed neuron/glial cultures stained with NBP1-05423, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-internexin NBP1-05207 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0001.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the solitary nuclear tract (center). Nissl Counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0006.jpg)
![Western Blot: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Western-Blot-NBP2-45406-img0014.jpg "Western Blot: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Peripherin.")
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0013.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0001.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0002.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0003.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0004.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0005.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0006.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0007.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0008.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0009.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0010.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Carcinoma of Human prostate. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0011.jpg)
![Immunohistochemistry: Peripherin Antibody (1E10) [NBP2-45406] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Peripherin-Antibody-1E10-Immunohistochemistry-NBP2-45406-img0012.jpg)
![Western Blot: Peripherin Antibody (8G2) [NB300-138] - Analysis of tissue and cell lysates probed with mouse mAb to peripherin, NB300-138, dilution 1:500 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] cow spinal cord, [5] SH-SY5Y cells, and [6] PC12 cells. The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.](http://images.novusbio.com/fullsize/Peripherin-Antibody-8G2-Western-Blot-NB300-138-img0002.jpg "Western Blot: Peripherin Antibody (8G2) [NB300-138] - Analysis of tissue and cell lysates probed with mouse mAb to peripherin, NB300-138, dilution 1:500 in green: [1] protein standard (red), [2] mouse spinal cord, [3] rat spinal cord, [4] cow spinal cord, [5] SH-SY5Y cells, and [6] PC12 cells. The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (8G2) [NB300-138] - Analysis of human neuroblastoma cell line SH-SY5Y (A) and rat pheochromocytoma cell line PC12 (B), stained with mouse mAb to peripherin, NB300-138, dilution 1:500, in red, and costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. Peripherin, one of the Class III family of intermediate filament (IF) subunit proteins is revealed by NB300-138 antibody in the perinuclear region in some SH-SY5Y cells and in all PC12 cells. Vimentin, a protein also in the Class III IF family, is detected in a subpopulation of SH-SY5Y cells which are negative for peripherin. PC12 cells express peripherin but not vimentin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-8G2-Immunocytochemistry-Immunofluorescence-NB300-138-img0001.jpg)
![Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Western-Blot-NB300-220-img0004.jpg "Western Blot: Peripherin Antibody (7C5) [NB300-220] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:1,000 (Green): [1] protein standard, [2] rat spinal cord, [3] rat sciatic nerve, [4] mouse spinal cord, [5] pig spinal cord, [6] cow spinal cord, [7] SH-SY5Y cells, and [8] PC12 cells. The band at ~57kDa corresponds to the peripherin protein detected only in rat and mouse lysates, since the antibody is rodent specific.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Analysis of mixed fibroblast and rat pheochromocytoma PC12 cell culture stained with peripherin antibody, dilution 1:500 (Red), and costained with chicken pAb to vimentin, dilution 1:10,000 (Green). Hoechst staining of nuclear DNA (Blue). The peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Immunocytochemistry-Immunofluorescence-NB300-220-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (7C5) [NB300-220] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-220 antibody to peripherin (green) and rabbit polyclonal antibody to NF-L NBP1-05218 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.](http://images.novusbio.com/fullsize/Peripherin-Antibody-7C5-Immunocytochemistry-Immunofluorescence-NB300-220-img0003.jpg)
![Western Blot: Peripherin Antibody (3B3) [H00005630-M02] - PRPH monoclonal antibody (M02), clone 3B3. Analysis of PRPH expression in IMR-32.](http://images.novusbio.com/fullsize/Peripherin-Antibody-3B3-Western-Blot-H00005630-M02-img0007.jpg "Western Blot: Peripherin Antibody (3B3) [H00005630-M02] - PRPH monoclonal antibody (M02), clone 3B3. Analysis of PRPH expression in IMR-32.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody (3B3) [H00005630-M02] - Analysis of monoclonal antibody to PRPH on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Peripherin-Antibody-3B3-Immunocytochemistry-Immunofluorescence-H00005630-M02-img0003.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody (3B3) [H00005630-M02] - Analysis of monoclonal antibody to PRPH on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Peripherin-Antibody-3B3-Immunohistochemistry-Paraffin-H00005630-M02-img0005.jpg)
![Sandwich ELISA: Peripherin Antibody (3B3) [H00005630-M02] - Detection limit for recombinant GST tagged PRPH is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Peripherin-Antibody-3B3-Sandwich-ELISA-H00005630-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-39008] - Staining of human cerebral cortex, pancreas, rectum and upper gastrointestinal using Anti-PRPH antibody NBP2-39008 (A) shows similar protein distribution across tissues to independent antibody NBP2-38444 (B).](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-39008-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-39008] - Staining of human cerebral cortex, pancreas, rectum and upper gastrointestinal using Anti-PRPH antibody NBP2-39008 (A) shows similar protein distribution across tissues to independent antibody NBP2-38444 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-39008] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-39008-img0010.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-39008] - Staining of human duodenum shows strong cytoplasmic positivity in the peripheral ganglion.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-39008-img0008.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-39008] - Staining of human rectum shows strong cytoplasmic positivity in the peripheral ganglion.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-39008-img0009.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-39008] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-39008-img0011.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-38444] - Staining of human cerebral cortex, pancreas, rectum and upper gastrointestinal using Anti-PRPH antibody NBP2-38444 (A) shows similar protein distribution across tissues to independent antibody NBP2-39008 (B).](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38444-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-38444] - Staining of human cerebral cortex, pancreas, rectum and upper gastrointestinal using Anti-PRPH antibody NBP2-38444 (A) shows similar protein distribution across tissues to independent antibody NBP2-39008 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-38444] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38444-img0011.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-38444] - Staining of human duodenum shows strong cytoplasmic positivity in the peripheral ganglion.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38444-img0009.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-38444] - Staining of human rectum shows strong cytoplasmic positivity in the peripheral ganglion.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38444-img0010.jpg)
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-38444] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-38444-img0012.jpg)
![Western Blot: Peripherin Antibody [NBP2-19774] - Sample (30 ug of whole cell lysate) A: PC-3 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NBP2-19774-img0001.jpg "Western Blot: Peripherin Antibody [NBP2-19774] - Sample (30 ug of whole cell lysate) A: PC-3 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Peripherin Antibody [NBP2-19774] - Peripherin antibody detects PRPH protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. Peripherin antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Paraffin-NBP2-19774-img0002.jpg)
![SDS-Page: Recombinant Human Peripherin Protein [H00005630-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue](http://images.novusbio.com/fullsize/Recombinant-Human-Peripherin-Protein-SDS-Page-H00005630-P01-img0002.jpg "SDS-Page: Recombinant Human Peripherin Protein [H00005630-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue")
