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![Western Blot: Pin1 Antibody [NBP1-05424] - Western blot of whole HeLa cell homogenate stained with NBP1-05424, at dilution of 1:10000. A prominent band running with an apparent SDS-PAGE molecular weight of approx. 21kDa corresponds to Pin1.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NBP1-05424-img0002.jpg "Western Blot: Pin1 Antibody [NBP1-05424] - Western blot of whole HeLa cell homogenate stained with NBP1-05424, at dilution of 1:10000. A prominent band running with an apparent SDS-PAGE molecular weight of approx. 21kDa corresponds to Pin1.")
![Immunocytochemistry/Immunofluorescence: Pin1 Antibody [NBP1-05424] - HeLa cells stained with NBP1-05424 antibody to Pin-1 (1:1000 dilution, green) and monoclonal to fibrillarin, NB300-269 (red). Pin-1 stains the nuclear matrix and, much more faintly, the cytoplasm. The fibrillarin antibody marks nucleoli.](http://images.novusbio.com/fullsize/Pin1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05424-img0003.jpg)
![Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells treated with scrambled (scr). Lane 2: T9ug cells treated with PIN1 kd1 shRNA. Lane 3: T9ug cells treated with PIN1-overexpressing plasmid HAP1. Lane 4: T9ug cells treated with PIN1-overexpressing plasmid HAP1S67E. Lane 5: T9ug cells treated with PIN1 kd2 shRNA. Load: 25 ug per lane. Primary antibody: PIN 1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted size: 18 kDa for PIN-1. Other band(s): normalized with a-tubulin (a-tub) antibody.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NBP1-77757-img0003.jpg "Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells treated with scrambled (scr). Lane 2: T9ug cells treated with PIN1 kd1 shRNA. Lane 3: T9ug cells treated with PIN1-overexpressing plasmid HAP1. Lane 4: T9ug cells treated with PIN1-overexpressing plasmid HAP1S67E. Lane 5: T9ug cells treated with PIN1 kd2 shRNA. Load: 25 ug per lane. Primary antibody: PIN 1 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted size: 18 kDa for PIN-1. Other band(s): normalized with a-tubulin (a-tub) antibody.")
![Immunohistochemistry: Pin1 Antibody [NBP1-77757] - Tissue: testis. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-PIN1 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIN-1 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/Pin1-Antibody-Immunohistochemistry-NBP1-77757-img0004.jpg)
![Western Blot: Pin1 Antibody [NBP1-77757] - Detection of endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5,000 dilution of HRP Gt-a-Rabbit IgG was used with a 15 sec exposure time.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NBP1-77757-img0001.jpg)
![Western Blot: Pin1 Antibody [NBP1-77757] - Lane 1: T9ug cells incubated with GST-Pin1. Lane 2: T9ug cells incubated with GST-CDK4/cyclinD1. Lane 3: T9ug cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 ug per lane. Primary antibody: anti-GST 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NBP1-77757-img0002.jpg)
![Western Blot: Pin1 Antibody (S01-7G8) [NBP3-15076] - Western blot detection of Pin1 in Jurkat, Rat Brain, C6, CHO-K1, Hela cell lysates using Pin1 Rabbit mAb (1:1000 diluted). Predicted band size: 18kDa. Observed band size: 18kDa.](http://images.novusbio.com/fullsize/Pin1-Antibody-S01-7G8-Western-Blot-NBP3-15076-img0001.jpg "Western Blot: Pin1 Antibody (S01-7G8) [NBP3-15076] - Western blot detection of Pin1 in Jurkat, Rat Brain, C6, CHO-K1, Hela cell lysates using Pin1 Rabbit mAb (1:1000 diluted). Predicted band size: 18kDa. Observed band size: 18kDa.")
![Immunohistochemistry-Paraffin: Pin1 Antibody (S01-7G8) [NBP3-15076] - Immunohistochemistry of Pin1 in paraffin-embedded Human breast cancer tissue using Pin1 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/Pin1-Antibody-S01-7G8-Immunohistochemistry-Paraffin-NBP3-15076-img0002.jpg)
![Western Blot: Pin1 Antibody [NBP2-55706] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NBP2-55706-img0002.jpg "Western Blot: Pin1 Antibody [NBP2-55706] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Pin1 Antibody [NBP2-55706] - Staining of human cell line HEK 293 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Pin1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55706-img0001.jpg)
![Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pin1-Antibody-Western-Blot-NB100-53805-img0001.jpg "Western Blot: Pin1 Antibody [NB100-53805] - (0.2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Western-Blot-NBP1-22956-img0002.jpg "Western Blot: Pin1 Antibody (3G8) [NBP1-22956] - Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Flow Cytometry: Pin1 Antibody (3G8) [NBP1-22956] - Analysis of Pin1 in Jurkat cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Pin1-Antibody-3G8-Flow-Cytometry-NBP1-22956-img0003.jpg)
![Western Blot: Pin1 Antibody (S06-4H6) [NBP3-15075] - Western blot detection of Pin1 in K562, Rat Brain, C6, 3T3 cell lysates using Pin1 Rabbit mAb (1:1000 diluted). Predicted band size: 18kDa. Observed band size: 18kDa.](http://images.novusbio.com/fullsize/Pin1-Antibody-S06-4H6-Western-Blot-NBP3-15075-img0001.jpg "Western Blot: Pin1 Antibody (S06-4H6) [NBP3-15075] - Western blot detection of Pin1 in K562, Rat Brain, C6, 3T3 cell lysates using Pin1 Rabbit mAb (1:1000 diluted). Predicted band size: 18kDa. Observed band size: 18kDa.")
![Immunoprecipitation: Pin1 Antibody [NBP1-28699] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PIN1 antibody NBP1-28699 used for IP at 10 ug/mg lysate. PIN1 was not successfully immunoprecipitated by rabbit anti-PIN1 antibody NBP1-28713, which recognizes a downstream epitope. For blotting immunoprecipitated PIN1, NBP1-28713 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Pin1-Antibody-Immunoprecipitation-NBP1-28699-img0002.jpg "Immunoprecipitation: Pin1 Antibody [NBP1-28699] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PIN1 antibody NBP1-28699 used for IP at 10 ug/mg lysate. PIN1 was not successfully immunoprecipitated by rabbit anti-PIN1 antibody NBP1-28713, which recognizes a downstream epitope. For blotting immunoprecipitated PIN1, NBP1-28713 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Pin1 Overexpression Lysate (Adult Normal) [NBL1-14428] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pin1.](http://images.novusbio.com/fullsize/Pin1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14428-img0002.jpg "Western Blot: Pin1 Overexpression Lysate (Adult Normal) [NBL1-14428] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pin1.")
![Sandwich ELISA: Pin1 Antibody (5A8) [H00005300-M02] - Detection limit for recombinant GST tagged PIN1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pin1-Antibody-5A8-Sandwich-ELISA-H00005300-M02-img0002.jpg "Sandwich ELISA: Pin1 Antibody (5A8) [H00005300-M02] - Detection limit for recombinant GST tagged PIN1 is approximately 0.1ng/ml as a capture antibody.")