| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC2457P | |||
| Carrier Free | IC2457A | |||
| Carrier Free | MAB2457-SP | |||
| Carrier Free | IC2457V-100UG | |||
| Carrier Free | IC2457U-100UG | |||
| Carrier Free | IC2457T-100UG | |||
| Carrier Free | IC2457S-100UG | |||
| Carrier Free | IC2457R-100UG | |||
| Carrier Free | IC2457N-100UG | |||
| Carrier Free | IC2457G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC2457P | |||
| Carrier Free | MAB2457 | |||
| Carrier Free | IC2457A | |||
| Carrier Free | MAB2457-SP | |||
| Carrier Free | IC2457V-100UG | |||
| Carrier Free | IC2457U-100UG | |||
| Carrier Free | IC2457T-100UG | |||
| Carrier Free | IC2457S-100UG | |||
| Carrier Free | IC2457R-100UG | |||
| Carrier Free | IC2457N-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1675-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3364-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Phycoerythrin | IC8150P-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8150-SP | |||
| Carrier Free | IC8150P-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48226 | |||
| Azide and BSA Free | NBP2-34705-0.1mg | |||
![Ablation of AAV-PdynP+ spinal dorsal horn (SDH) neurons does not affect behavioral responses evoked by the optical stimulation of A beta fibers, nociceptive mechanical force, and heat in rats. (A) Enhanced green fluorescent protein (EGFP) expression (green) in the L4 SDH at 4 weeks after intra-SDH microinjection of AAV-PdynP-DTR-EGFP (indicated by an upper schematic illustration). Colocalization of EGFP and PAX2 (red) in the SDH (indicated by arrowheads). Scale bar, 100 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 94 total EGFP+ cells quantified, and 339 total PAX2+ cells quantified). (B) Diphtheria toxin receptors (DTR) immunofluorescence in the SDH after administration of diphtheria toxin (DTX: 50 μg/kg, i.p., two injection 72 h apart) or PBS. Scale bar, 200 μm. (C) Immunohistochemical analysis and quantification of PAX2+ neurons in the superficial laminae of the ipsilateral and contralateral SDH at 4 weeks after DTX injection (n = 6 rats tested, PAX2+ neurons: 992 [ipsi] and 1574 [contra] cells quantified). Scale bar, 50 μm. ****P < 0.0001, Unpaired t-test. (D) Paw withdrawal behaviors by light (score), von Frey filaments (threshold), and heat (latency) at 4 weeks after DTX or PBS injection (n = 7–9 rats). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3364_human-pax2-affinity-purified-polyclonal-ab-22820241721190.jpg)
![Ablation of AAV-PdynP+ spinal dorsal horn (SDH) neurons does not affect behavioral responses evoked by the optical stimulation of A beta fibers, nociceptive mechanical force, and heat in rats. (A) Enhanced green fluorescent protein (EGFP) expression (green) in the L4 SDH at 4 weeks after intra-SDH microinjection of AAV-PdynP-DTR-EGFP (indicated by an upper schematic illustration). Colocalization of EGFP and PAX2 (red) in the SDH (indicated by arrowheads). Scale bar, 100 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 94 total EGFP+ cells quantified, and 339 total PAX2+ cells quantified). (B) Diphtheria toxin receptors (DTR) immunofluorescence in the SDH after administration of diphtheria toxin (DTX: 50 μg/kg, i.p., two injection 72 h apart) or PBS. Scale bar, 200 μm. (C) Immunohistochemical analysis and quantification of PAX2+ neurons in the superficial laminae of the ipsilateral and contralateral SDH at 4 weeks after DTX injection (n = 6 rats tested, PAX2+ neurons: 992 [ipsi] and 1574 [contra] cells quantified). Scale bar, 50 μm. ****P < 0.0001, Unpaired t-test. (D) Paw withdrawal behaviors by light (score), von Frey filaments (threshold), and heat (latency) at 4 weeks after DTX or PBS injection (n = 7–9 rats). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3364_human-pax2-affinity-purified-polyclonal-ab-22820241723636.jpg)
![Western Blot: PAX8 Antibody [NBP1-32440] - 293T whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP1-32440-img0019.jpg "Western Blot: PAX8 Antibody [NBP1-32440] - 293T whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs). Schematic description of the two-stage protocol applied to iPSC-renal commitment. Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. Pictured are the IM and MM marker expressions as Wt1, Pax8, Pax2, Six2 and Sall1. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32440-img0020.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Pax8 immunoreactivity in canine renal carcinoma. Primary antibody incubated with tissue for 1h at room temperature. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0021.jpg)
![Chromatin Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of PAX8 antibody The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.](http://images.novusbio.com/fullsize/PAX8-Antibody-Chromatin-Immunoprecipitation-NBP1-32440-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32440-img0011.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Rat thyroid gland. PAX8 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0015.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - DLD1 xenograft . PAX8 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0016.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Mouse liver. PAX8 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunohistochemistry-Paraffin-NBP1-32440-img0018.jpg)
![Immunoprecipitation: PAX8 Antibody [NBP1-32440] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of PAX8 protein by 2 ug of PAX8 antibody 10% SDS-PAGE gel.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunoprecipitation-NBP1-32440-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP1-32440] - Differentiation of human iPSC clone IV towards renal commitment.(a) Representative immunofluorescence images of co-staining for Lhx1/Osr1 up to day 6. (b) Images of co-staining of Pax2/Six2, Pax8/Six2 & Six2/Wt1 from day 6 to 12. (c) Expression of renal progenitor markers such as CD24, Claudin1 & GGT1 from day 0 to 19. Nuclei are stained with DAPI (blue). Scale bars: 50 μm (a, b), 20 μm (c). (d) Gene expression analysis for renal progenitor markers at different points in time. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-310202415345341.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - PDX model establishment from ccRCC injection. a Hematoxylin & Eosin staining of human tumor engrafted in murine models. A epresentative image of kidney PDX reported. Microscope Nikon Eclipse E1000 10X & 20X (b) Graph reporting % of patients who engrafted when orthotopically injected in mice & distributed following grading (3 G1; 7 G2; 13 G3; 7 G4). Eight mice for each patient injected & all 18 tumors on 30 which evaluated as engrafted developed tumor masses on ≥ of 6 mice. Tumors declared unable to engraft did not produce, at all, tumor masses. c Representative images (1 × 0.63) of PDXs excised 90 days after injection by Stereomicroscope (Olympus SZX10,XCX50). One representative image for G2, G3 & G4 types reported. d Representative images (1 × 0.63 & 1.25) of aberrant neo-angiogenesis formation in PDXs by Stereomicroscope (Olympus SZX10, XCX50 camera). e Hematoxylin & Eosin staining of PDXs versus parental primary patients. One representative tumor for each grade (G2, G3, G4) reported. Staining executed on OCT frozen samples. f Hematoxylin & Eosin & anti-PAX8, CD10, Vimentin & EMA staining reported on formalin-fixed & paraffin- embedded parental primary, metastatic tissues & PDXs. Primary, metastatic & PDX tissues obtained & shown from one representative patient. Microscope Nikon Eclipse 55i, magnification 20×. g Histogram showing number of engrafting tumor populations evaluated over 30 injected patient samples & correlated w/ patient recurrence frequency calculated as development of metastases after surgery. Orange color represents recurrent (n = 7) & metastatic (n = 3) patients in engrafted group (n = 18). Pink color represents recurrent patients (n = 2) in non-engrafted group (n = 12) for a total of 30 injected samples Image collected & cropped by CiteAb from following publication (//pubmed.ncbi.nlm.nih.gov/30185225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-310202416165612.jpg)
![Western Blot: PAX8 Antibody [NBP1-32440] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (NBP1-32440) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-910202420134072.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - PAX8 antibody detects PAX8 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.PAX8 stained by PAX8 antibody (NBP1-32440) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-910202419593589.jpg)
![Western Blot: PAX8 Antibody [NBP1-32440] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (NBP1-32440) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32440_rabbit-polyclonal-pax8-antibody-9102024201466.jpg)
![Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Western-Blot-H00005076-M01-img0006.jpg "Western Blot: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of PAX2 expression in transfected 293T cell line by PAX2 monoclonal antibody (M01), clone 3C7.Lane 1: PAX2 transfected lysate(47.41 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Pax2 Antibody (3C7) [H00005076-M01] - Analysis of monoclonal antibody to PAX2 on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-Immunohistochemistry-Paraffin-H00005076-M01-img0004.jpg)
![ELISA: Pax2 Antibody (3C7) [H00005076-M01] - Detection limit for recombinant GST tagged PAX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pax2-Antibody-3C7-ELISA-H00005076-M01-img0007.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0023.jpg "Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-NB300-750-img0024.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0017.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0016.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0019.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-750-img0011.jpg)
![Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunoprecipitation-NB300-750-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) & IM marker Osr1. (c) IM & MM marker expression as Wt1, Pax8, Pax2, Six2 & Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 & proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, & cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-750_rabbit-polyclonal-pax6-antibody-3102024161898.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0011.jpg "Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.")
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-Immunofluorescence-NBP2-29903-img0005.jpg)
![Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Staining in mouse thyroid tissue at pre-tumor stage (red). Dilution is 1:100. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-NBP2-29903-img0012.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 60ug of SKOV-3, COS7, and negative control HeLa whole cell lysates, and 10ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0009.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 25ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane), and 10ul PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0010.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of mouse kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0006.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0007.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0008.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0023.jpg "Western Blot: Pax6 Antibody [NBP2-19711] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP2-19711] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAX6 protein stained by PAX6 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19711-img0013.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Mouse retina. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP2-19711-img0021.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0015.jpg)
![Western Blot: Pax6 Antibody [NBP2-19711] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NBP2-19711-img0017.jpg)
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0010.jpg)
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0012.jpg)
![Immunoprecipitation: PAX6 Antibody [NBP2-19711] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ug of PAX6 antibody 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PAX6-Antibody-Immunoprecipitation-NBP2-19711-img0004.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420104458.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202419593540.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420115034.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242075957.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242001050.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Pax6 Antibody [NBP2-19711] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-161020241941454.jpg)
![Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.](http://images.novusbio.com/fullsize/Pax6-Antibody-PAX6-498-Western-Blot-NBP2-32893-img0001.jpg "Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.")
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of HEK293 lysate overexpressing PAX8 (right lane) or empty vector control (left lane) and 10ul PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0011.jpg "Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of HEK293 lysate overexpressing PAX8 (right lane) or empty vector control (left lane) and 10ul PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP2-29907] - Analysis showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29907-img0007.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 75 ug of various whole cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0009.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 60ug of COS7 whole cell lysate (left lane), negative control HeLa lysate (right lane), and 10ul PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0010.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 polyclonal antibody at a dilution of 1:400 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29907-img0006.jpg)
![Immunoprecipitation: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 was performed using COS7 whole cell lysate. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of a PAX8 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with a PAX8 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. Membranes were washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunoprecipitation-NBP2-29907-img0008.jpg)