![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0011.jpg "Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.")
| Reactivity | Hu, Mu, Pm, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 1F8-3A8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Purified internal fragment of human recombinant PAX8 expressed in E. coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PAX8 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Detects a prominent approx. 48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8. IHC reported in a review from a verified customer. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA and 30% glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Feng Li |
ICC | Mouse | 05/30/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for PAX8 Antibody (NBP2-29903)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-Immunofluorescence-NBP2-29903-img0005.jpg "Immunocytochemistry/Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.")
![Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Staining in mouse thyroid tissue at pre-tumor stage (red). Dilution is 1:100. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-NBP2-29903-img0012.jpg "Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Staining in mouse thyroid tissue at pre-tumor stage (red). Dilution is 1:100. This image was submitted via customer Review.")
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 60ug of SKOV-3, COS7, and negative control HeLa whole cell lysates, and 10ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0009.jpg "Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 60ug of SKOV-3, COS7, and negative control HeLa whole cell lysates, and 10ul of PageRuler Plus Prestained Protein Ladder.")
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 25ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane), and 10ul PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0010.jpg "Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 25ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane), and 10ul PageRuler Plus Prestained Protein Ladder.")
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of mouse kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0006.jpg "Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of mouse kidney tissue (right) compared to a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0007.jpg "Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).")
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0008.jpg "Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of human kidney tissue (right) compared to a negative control without primary antibody (left).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
