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![Western Blot: Nrf1 Antibody [NBP1-89125] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-89125-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nrf1 Antibody [NBP1-89125] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Nrf1 Antibody [NBP1-89125] - The SIAH2-NRF1 axis facilitates hypoxia-induced metabolic reprogramming. Wild-type or K230R stably expressed MDA-MB-231 cells were cultured under normoxia or hypoxia for 36 h. Cells were harvested and analyzed by western blotting with indicated antibodies. Right: densitometric quantification of the indicated proteins. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-08618-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-89125-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP1-89125] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89125-img0005.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0011.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0009.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP1-89125] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP1-89125-img0010.jpg)
![Western Blot: Nrf1 Antibody [NBP2-19586] - A. 30 ug 293T whole cell lysate/extractB. 30 ug 293T nuclear lysate/extract C. 30 ug A431 whole cell lysate/extract | D. 30 ug A431 nuclear lysate/extract E. 30 ug HeLa whole cell lysate/extract F. 30 ug HeLa nuclear lysate/extract G. 30 ug HepG2 whole cell lysate/extract H. 30 ug HepG2 nuclear lysate/extract10 % SDS-PAGE NRF1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP2-19586-img0008.jpg "Western Blot: Nrf1 Antibody [NBP2-19586] - A. 30 ug 293T whole cell lysate/extractB. 30 ug 293T nuclear lysate/extract C. 30 ug A431 whole cell lysate/extract | D. 30 ug A431 nuclear lysate/extract E. 30 ug HeLa whole cell lysate/extract F. 30 ug HeLa nuclear lysate/extract G. 30 ug HepG2 whole cell lysate/extract H. 30 ug HepG2 nuclear lysate/extract10 % SDS-PAGE NRF1 antibody dilution: 1:1000")
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0010.jpg)
![Western Blot: Nrf1 Antibody [NBP2-19586] - IP samples: 293T whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of NRF1 protein by 4 ug NRF1 antibody. 10 % SDS-PAGE The immunoprecipitated NRF1 protein was detected by NRF1 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent].](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP2-19586-img0003.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded human lung adenocarcinoma. NRF1 antibody diluted at 1:250.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0006.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse muscle. NRF1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP2-19586] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF1 stained by NRF1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Scale bar= 10μm.](http://images.novusbio.com/fullsize/antibody/nbp2-19586_rabbit-polyclonal-nrf1-antibody-immunocytochemistry-immunofluorescence-582024111857..jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Nrf1 antibody detects Nrf1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Nrf1 stained by Nrf1 antibody (NBP2-19586) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19586_rabbit-polyclonal-nrf1-antibody-91020242091460.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0012.jpg "Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate (Predicted MW: 55.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of monoclonal antibody to NRF1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Immunohistochemistry-Paraffin-H00004899-M01-img0007.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - Analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # H00004899-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0008.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in HeLa.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0010.jpg)
![Western Blot: Nrf1 Antibody (2F9) [H00004899-M01] - NRF1 monoclonal antibody (M01), clone 2F9. Analysis of NRF1 expression in human Skeletal muscle.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-Western-Blot-H00004899-M01-img0011.jpg)
![ELISA: Nrf1 Antibody (2F9) [H00004899-M01] - Detection limit for recombinant GST tagged NRF1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nrf1-Antibody-2F9-ELISA-H00004899-M01-img0009.jpg)
![Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Western-Blot-NBP3-16161-img0003.jpg "Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Immunohistochemistry of paraffin-embedded human colon using Nrf1 Rabbit mAb (NBP3-16161) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Immunohistochemistry-Paraffin-NBP3-16161-img0001.jpg)
![Western Blot: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Western blot analysis of extracts of various cell lines, using Nrf1 Rabbit mAb (NBP3-16161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Nrf1-Antibody-4Q4E7-Western-Blot-NBP3-16161-img0002.jpg)
![Chromatin Immunoprecipitation: Nrf1 Antibody (4Q4E7) [NBP3-16161] - Analysis of extracts of HepG2 cells, using NRF1 antibody (A3252) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16161_rabbit-nrf1-mab-4q4e7-chromatin-immunoprecipitation-193202413322..jpg)
![ChIP sequencing-Nrf1 Antibody (4Q4E7) [NBP3-16161] - CUT&Tag was performed using the CUT&Tag Assay Kit for Illumina from 10⁵ HepG2 cells with 1 μg NRF1 Rabbit mAb along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of NRF1 in representative gene loci (LINS1), as shown in figure.](http://images.novusbio.com/fullsize/nbp3-16161_rabbit-nrf1-mab-4q4e7-chromatin-immunoprecipitation-sequencing-203202463743..jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0003.jpg "Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.")
![Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-NBP1-77822-img0004.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0002.jpg)
![Western Blot: Nrf1 Antibody (S02-9A7) [NBP3-14960] - Western blot detection of Nrf1 in C6, 3T3, Hela cell lysates using Nrf1 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 68kDa.](http://images.novusbio.com/fullsize/Nrf1-Antibody-S02-9A7-Western-Blot-NBP3-14960-img0001.jpg "Western Blot: Nrf1 Antibody (S02-9A7) [NBP3-14960] - Western blot detection of Nrf1 in C6, 3T3, Hela cell lysates using Nrf1 Rabbit mAb (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 68kDa.")
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody (S02-9A7) [NBP3-14960] - Immunofluorescence of Nrf1 (green) in Hela cells using Nrf1 Rabbit mAb at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/Nrf1-Antibody-S02-9A7-Immunocytochemistry-Immunofluorescence-NBP3-14960-img0002.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody (S02-9A7) [NBP3-14960] - Immunohistochemistry of Nrf1 in paraffin-embedded Human colon cancer tissue using Nrf1 Rabbit mAb at dilution 1/5](http://images.novusbio.com/fullsize/Nrf1-Antibody-S02-9A7-Immunohistochemistry-Paraffin-NBP3-14960-img0003.jpg)
![Western Blot: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Azide-and-BSA-Free-Western-Blot-NBP3-08773-img0003.jpg "Western Blot: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08773-img0002.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) - Azide and BSA Free [NBP3-08773] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08773-img0001.jpg)
![Western Blot: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Western-Blot-NBP3-07451-img0003.jpg "Western Blot: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).")
![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Immunohistochemistry-Paraffin-NBP3-07451-img0002.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2609-Immunohistochemistry-Paraffin-NBP3-07451-img0001.jpg)
![Western Blot: Nrf1 Antibody (C.15200013) [NBP2-59255] - Whole cell extracts from K562 cells were analyzed using the antibody against diluted 1:1000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Nrf1-Antibody-C-15200013-Western-Blot-NBP2-59255-img0002.jpg "Western Blot: Nrf1 Antibody (C.15200013) [NBP2-59255] - Whole cell extracts from K562 cells were analyzed using the antibody against diluted 1:1000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Nrf1 Antibody (C.15200013) [NBP2-59255] - ChIP was performed using K562 cells, the antibody against NRF1 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for the BRCA1 and AURKA promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Nrf1-Antibody-C-15200013-Chromatin-Immunoprecipitation-NBP2-59255-img0001.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2608) [NBP3-07450] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2608).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2608-Immunohistochemistry-Paraffin-NBP3-07450-img0002.jpg "Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2608) [NBP3-07450] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2608).")
![Protein Array: Nrf1 Antibody (NRF1/2608) [NBP3-07450] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Nrf1 Mouse Monoclonal Antibody (Nrf1/2608).](http://images.novusbio.com/fullsize/Nrf1-Antibody-NRF1-2608-Protein-Array-NBP3-07450-img0001.jpg)
![SDS-Page: Recombinant Human Nrf1 Protein [H00004899-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Nrf1-Protein-H00004899-P01-img0001.jpg "SDS-Page: Recombinant Human Nrf1 Protein [H00004899-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")