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![Western Blot: NNT Antibody [NBP1-32109] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NNT-Antibody-Western-Blot-NBP1-32109-img0016.jpg "Western Blot: NNT Antibody [NBP1-32109] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse kidney. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-32109-img0010.jpg)
![Western Blot: NNT Antibody [NBP1-32109] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NNT-Antibody-Western-Blot-NBP1-32109-img0012.jpg)
![Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - SW480 xenograft, using NNT antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-32109-img0007.jpg)
![Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Mouse stomach. NNT antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-32109-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: NNT Antibody [NBP1-32109] - NNT antibody detects NNT protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: NNT stained by NNT antibody (NBP1-32109) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp1-32109_rabbit-polyclonal-nnt-antibody-91020242001076.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: NNT Antibody [NBP1-32109] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32109_rabbit-polyclonal-nnt-antibody-1610202419414517.jpg)
![Western Blot: NNT Antibody [NBP1-86512] - Analysis in human cell lines HeLa and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NNT-Antibody-Western-Blot-NBP1-86512-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: NNT Antibody [NBP1-86512] - Analysis in human cell lines HeLa and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512] - Staining in human heart muscle and skin tissues using NBP1-86512 antibody. Corresponding NNT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-86512-img0014.jpg)
![Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-86512-img0010.jpg)
![Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-86512-img0011.jpg)
![Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512] - Staining of human skin shows very weak positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-86512-img0012.jpg)
![Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP1-86512-img0013.jpg)
![Western Blot: NNT Antibody (1D6) [H00023530-M01] - NNT monoclonal antibody (M01), clone 1D6 Analysis of NNT expression in HepG2.](http://images.novusbio.com/fullsize/NNT-Antibody-1D6-Western-Blot-H00023530-M01-img0008.jpg "Western Blot: NNT Antibody (1D6) [H00023530-M01] - NNT monoclonal antibody (M01), clone 1D6 Analysis of NNT expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: NNT Antibody (1D6) [H00023530-M01] - Analysis of monoclonal antibody to NNT on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NNT-Antibody-1D6-Immunocytochemistry-Immunofluorescence-H00023530-M01-img0006.jpg)
![ELISA: NNT Antibody (1D6) [H00023530-M01] - Detection limit for recombinant GST tagged NNT is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NNT-Antibody-1D6-ELISA-H00023530-M01-img0003.jpg)
![Sandwich ELISA: NNT Antibody (1D6) [H00023530-M01] - Detection limit for recombinant GST tagged NNT is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NNT-Antibody-1D6-Sandwich-ELISA-H00023530-M01-img0007.jpg)
![Western Blot: NNT Antibody [NBP1-59612] - Sample Tissue: Hela Whole cell, Lane A: Primary Antibody, Lane B:Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/Lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/NNT-Antibody-Western-Blot-NBP1-59612-img0004.jpg "Western Blot: NNT Antibody [NBP1-59612] - Sample Tissue: Hela Whole cell, Lane A: Primary Antibody, Lane B:Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/Lane, Gel Concentration: 0.12")
![Western Blot: NNT Antibody [NBP1-59612] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/NNT-Antibody-Western-Blot-NBP1-59612-img0003.jpg)
![Western Blot: NNT Antibody [NBP2-99302] - Anti-NNT rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 114 kDa](http://images.novusbio.com/fullsize/NNT-Antibody-Western-Blot-NBP2-99302-img0003.jpg "Western Blot: NNT Antibody [NBP2-99302] - Anti-NNT rabbit polyclonal antibody at 1:500 dilution. Lane A: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 114 kDa")
![Immunohistochemistry-Paraffin: NNT Antibody [NBP2-99302] - Immunochemical staining of human NNT in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP2-99302-img0002.jpg)
![Western Blot: NNT Overexpression Lysate (Adult Normal) [NBL1-13701] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NNT.](http://images.novusbio.com/fullsize/NNT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13701-img0002.jpg "Western Blot: NNT Overexpression Lysate (Adult Normal) [NBL1-13701] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NNT.")
![Western Blot: NNT Overexpression Lysate (Adult Normal) [NBP2-04791] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NNT.](http://images.novusbio.com/fullsize/NNT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04791-img0001.jpg "Western Blot: NNT Overexpression Lysate (Adult Normal) [NBP2-04791] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NNT.")
![Immunohistochemistry-Paraffin: NNT Antibody [NBP2-98006] - Immunochemical staining of human NNT in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP2-98006-img0002.jpg "Immunohistochemistry-Paraffin: NNT Antibody [NBP2-98006] - Immunochemical staining of human NNT in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: NNT Antibody [NBP2-98006] - Immunochemical staining of human NNT in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NNT-Antibody-Immunohistochemistry-Paraffin-NBP2-98006-img0001.jpg)