| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-53421SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-862-0.1mg | |||
| HRP | NB600-863-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59441-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03453-20ul | |||
![Western Blot: Lactate Dehydrogenase B Antibody [NBP2-53421] - Western blot shows lysates of 293 human embryonic kidney parental cell line and LDHB knockout (KO) 293 cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human LDHB Polyclonal Antibody (NBP2-53421) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for LDHB at approximately 35 kDa (as indicated) in the parental 293 cell line, but is not detectable in the knockout 293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Knockout-Validated-NBP2-53421-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lactate Dehydrogenase B Antibody [NBP2-53421] - Western blot shows lysates of 293 human embryonic kidney parental cell line and LDHB knockout (KO) 293 cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human LDHB Polyclonal Antibody (NBP2-53421) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for LDHB at approximately 35 kDa (as indicated) in the parental 293 cell line, but is not detectable in the knockout 293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Lactate Dehydrogenase B Antibody [NBP2-53421] - Total protein from human HeLa and A431 cells, mouse MEF cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LDHB in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP2-53421-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-LDHB at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53421-img0006.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP2-53421] - Analysis of a FFPE tissue section of mouse liver using Lactate Dehydrogenase B antibody at 1:400 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated a very specific staining in the hepatocytes.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-Paraffin-NBP2-53421-img0002.jpg)
![Flow Cytometry: Lactate Dehydrogenase B Antibody [NBP2-53421] - An intracellular stain was performed on SK-MEL-28 cells with Lactate Dehyrdrogenase B Antibody NBP2-53421F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Flow-Cytometry-NBP2-53421-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-LDHB at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53421-img0001.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Sample Tissue: Human Fetal Lung, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0011.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Sample Tissue: Human Fetal Lung, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-55415] - Human lung adenocarcinoma cell line A549, 2.5.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-55415-img0003.jpg)
![Immunohistochemistry: Lactate Dehydrogenase B Antibody [NBP1-55415] - Human Placenta Antibody Concentration: 5 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-NBP1-55415-img0006.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Human pancreatic cancer cell line MiaPaca-2 and Panc-1, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0001.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - 721_B Whole Cell, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0002.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Antibody Titration: 1 ug/ml Human 721_B.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0007.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Antibody Titration: 1 ug/ml Human Raji.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0008.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Lanes: 1. 30 ug MiaPaca-2 cell lysate 2. 30 ug Panc-1 cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 4000 Gene name: LDHB.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0010.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-55415] - Lane A: Human Fetal Lung Lane B: Human HCT116 Lane C: Human 293T, Antibody Dilution: 0.2 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-55415-img0009.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - LDHB monoclonal antibody (M01), clone 2H6 Analysis of LDHB expression in Hela.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-2H6-Western-Blot-H00003945-M01-img0009.jpg "Western Blot: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - LDHB monoclonal antibody (M01), clone 2H6 Analysis of LDHB expression in Hela.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - Analysis of monoclonal antibody to LDHB on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-2H6-Immunocytochemistry-Immunofluorescence-H00003945-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - Analysis of monoclonal antibody to LDHB on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-2H6-Immunohistochemistry-Paraffin-H00003945-M01-img0007.jpg)
![ELISA: Lactate Dehydrogenase B Antibody (2H6) [H00003945-M01] - Detection limit for recombinant GST tagged LDHB is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-2H6-ELISA-H00003945-M01-img0008.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody(diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP1-31945 was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-31945-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody(diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP1-31945 was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-31945-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-31945] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31945-img0002.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945] - Mouse liver. LDH-B stained by LDH-B antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-31945-img0015.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP1-31945-img0010.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945] - Human gastric cancer. LDH-B antibody dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-31945-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31945_rabbit-polyclonal-lactate-dehydrogenase-b-antibody-161020241940577.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0012.jpg "Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NB600-861-img0015.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0009.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0010.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0011.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody [NBP2-38131] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP2-38131-img0003.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP2-38131] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-38131] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38131-img0005.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP2-38131] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Immunohistochemistry-Paraffin-NBP2-38131-img0004.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Western blot analysis of extracts of various cell lines, using Lactate Dehydrogenase B Rabbit mAb (NBP3-16569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-6B5I2-Western-Blot-NBP3-16569-img0003.jpg "Western Blot: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Western blot analysis of extracts of various cell lines, using Lactate Dehydrogenase B Rabbit mAb (NBP3-16569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Immunohistochemistry of paraffin-embedded human appendix using Lactate Dehydrogenase B Rabbit mAb (NBP3-16569) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-6B5I2-Immunohistochemistry-Paraffin-NBP3-16569-img0002.jpg)
![Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Immunohistochemistry of paraffin-embedded rat ovary using Lactate Dehydrogenase B Rabbit mAb (NBP3-16569) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-6B5I2-Immunohistochemistry-Paraffin-NBP3-16569-img0001.jpg)
![Immunoprecipitation: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Lactate Dehydrogenase B antibody (NBP3-16569). Western blot was performed from the immunoprecipitate using Lactate Dehydrogenase B antibody (NBP3-16569) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-6B5I2-Immunoprecipitation-NBP3-16569-img0004.jpg)
![Western Blot: Lactate Dehydrogenase B Antibody (SR1618) [NBP3-22173] - Western blot analysis using NBP3-22173 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22173_rabbit-lactate-dehydrogenase-b-mab-sr1618-572023156477.jpg "Western Blot: Lactate Dehydrogenase B Antibody (SR1618) [NBP3-22173] - Western blot analysis using NBP3-22173 in HeLa cell lysate.")
![Western Blot: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Lane 1: Recombinant LDHB protein, Lane 2: A549 cell lysates, Lane 3: 293T cell lysates, Lane 4: K562 cell lysates, Lane 5: Jurkat cell lysates](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-14D7-Western-Blot-NBP2-59441-img0001.jpg "Western Blot: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Lane 1: Recombinant LDHB protein, Lane 2: A549 cell lysates, Lane 3: 293T cell lysates, Lane 4: K562 cell lysates, Lane 5: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody (14D7) [NBP2-59441] - Analysis of LDHB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LDHB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-14D7-Immunocytochemistry-Immunofluorescence-NBP2-59441-img0002.jpg)
![Immunoprecipitation:Lactate Dehydrogenase AntibodyNBP3-30578] -Detection of human Lactate Dehydrogenase B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody NBP3-30578 (lot NBP3-30578-1) used for IP at 6 µg per reaction. Lactate Dehydrogenase B was also immunoprecipitated by rabbit anti-Lactate Dehydrogenase B antibody BL18400. For blotting immunoprecipitated Lactate Dehydrogenase B, NBP3-30578 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30578_rabbit-lactate-dehydrogenase-pab-301220241740583.jpg "Immunoprecipitation:Lactate Dehydrogenase AntibodyNBP3-30578] -Detection of human Lactate Dehydrogenase B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody NBP3-30578 (lot NBP3-30578-1) used for IP at 6 µg per reaction. Lactate Dehydrogenase B was also immunoprecipitated by rabbit anti-Lactate Dehydrogenase B antibody BL18400. For blotting immunoprecipitated Lactate Dehydrogenase B, NBP3-30578 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: Lactate Dehydrogenase B Antibody [NBP3-03453] - Analysis of extracts of various cell lines, using Lactate Dehydrogenase B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-B-Antibody-Western-Blot-NBP3-03453-img0002.jpg "Western Blot: Lactate Dehydrogenase B Antibody [NBP3-03453] - Analysis of extracts of various cell lines, using Lactate Dehydrogenase B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")