| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5787-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB57871-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5787-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-212SS | |||
![Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Loss of Elf3 preserves the mesenchymal state. Immunoblot showing ELF3 and E-cadherin protein levels in response to Elf3 silencing by siRNAs. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/8/858) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Western-Blot-NBP1-30873-img0010.jpg "Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Loss of Elf3 preserves the mesenchymal state. Immunoblot showing ELF3 and E-cadherin protein levels in response to Elf3 silencing by siRNAs. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/8/858) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: ELF3/ESE-1 Antibody [NBP1-30873] - Methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30873-img0006.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - Human lung carcinoma. ESE1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30873-img0009.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - NCI-N87 xenograft, using ESE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30873-img0007.jpg)
![Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody [NBP1-30873] - The transcription factor ELF3 regulates the Grhl3 promoter. (A) The sequence of the Grhl3 promoter (1000 bp) showing the location of the predicted TATA box, SP1 sequence, & the transcription start site. Sequences in red represent the conserved regions, & green bases represent putative ELF3 binding sites. (B) Luciferase reporter assay showing the activation of the Grhl3 promoter by ELF3. Relative luciferase activity is calculated as fold induction relative to vector. Drawings on the left side show a schematic representation of the Grhl3 promoter region (top) & the structure of each reporter plasmid (below). The conserved regions are shown as horizontal boxes labeled 1,2 & 3, the restriction enzymes used to generate the reporter deletions of the promoter are labeled. (C) ELF3 binding at the promoter of Grhl3. NMuMG cells were treated with either vehicle or TGF beta 3 for 72 h, TGF beta 3 treated cells were then washed with PBS & continued incubation for an additional 48 h to initiate MET (post-treatment: PT48). Cells were cross-linked, & chromatin immunoprecipitation was performed. ChIP DNA was used in qPCR to measure the occupancy of ELF3 compared to control antibodies. (D) Sequence alignment of one putative ELF3 binding site in the Grhl3 promoter showing conservation among different species. In B & C, data represent the averages of at least three independent experiments. The paired Student’s t-test was used to calculate statistical significance. ** p-value < 0.01; **** p-value < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31404945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30873_rabbit-polyclonal-elf3-ese-1-antibody-310202416182629.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ESE1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-30873_rabbit-polyclonal-elf3-ese-1-antibody-161020241941455.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human colon and skeletal muscle tissues. Corresponding ELF3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87945-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human colon and skeletal muscle tissues. Corresponding ELF3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon, endometrium, pancreas and skeletal muscle using Anti-ELF3/ESE-1 antibody NBP1-87945 (A) shows similar protein distribution across tissues to independent antibody NBP1-87946 (B).](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87945-img0019.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ELF3/ESE-1 Antibody [NBP1-87945] - Analysis in human cell lines A-431 and SK-MEL-30 using anti-ELF3 antibody. Corresponding ELF3 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Western-Blot-NBP1-87945-img0012.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human pancreas shows moderate nuclear positivity in pancreatic ducts.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87945-img0008.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87945-img0016.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human colon shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87945-img0017.jpg)
![Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-87945] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87945-img0018.jpg)
![Western Blot: ESE1 Antibody [NB600-212] - WB detection of ESE1 in T84 colon carcinoma cells with NB600-212 antibody at a dilution of 1:500. Please note that this antibody detects both isoforms of ESE1 with expected molecular weight of 41.4 kDa and 39.3 kDa.](http://images.novusbio.com/fullsize/ESE1-Antibody-Western-Blot-NB600-212-img0001.jpg "Western Blot: ESE1 Antibody [NB600-212] - WB detection of ESE1 in T84 colon carcinoma cells with NB600-212 antibody at a dilution of 1:500. Please note that this antibody detects both isoforms of ESE1 with expected molecular weight of 41.4 kDa and 39.3 kDa.")
![Western Blot: ESE1 Antibody [NB600-212] - Western blot analysis of ESE1 in T84 colon carcinoma cells using NB600-212 at 1:500 dilution. Recognizes both isoforms of ESE1.](http://images.novusbio.com/fullsize/ESE1-Antibody-Western-Blot-NB600-212-img0002.jpg)
![Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Western blot analysis of extracts of various cell lines, using ELF3/ESE-1 Rabbit mAb (NBP3-16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ELF3-ESE-1-Antibody-7O2V4-Western-Blot-NBP3-16429-img0001.jpg "Western Blot: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Western blot analysis of extracts of various cell lines, using ELF3/ESE-1 Rabbit mAb (NBP3-16429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Chromatin Immunoprecipitation: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] - Analysis of extracts of HepG2 cells, using ESE1/ELF3 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16429_rabbit-elf3-ese-1-mab-7o2v4-chromatin-immunoprecipitation-chip--2492024124532..jpg)
![Chip IP Sequencing: ELF3/ESE-1 Antibody (7O2V4) [NBP3-16429] -CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HepG5 with 1μg ESE1 along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (MARK4), as shown in figure.](http://images.novusbio.com/fullsize/nbp3-16429_rabbit-elf3-ese-1-mab-7o2v4-chromatin-immunoprecipitation-sequencing-2492024131643..jpg)
![Western Blot: ELF3/ESE-1 Antibody (JE33-30) [NBP3-32320] - Western blot analysis of ELF3/ESE-1 on different lysates with Rabbit anti-ELF3/ESE-1 antibody (NBP3-32320) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 ug/Lane) Lane 2: SK-Br-3 cell lysate (10 ug/Lane) Lane 3: A431 cell lysate (10 ug/Lane) Lane 4: Mouse lung tissue lysate (20 ug/Lane) Lane 5: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 41.5 kDa Observed band size: 41.5 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32320) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32320_rabbit-elf3-ese-1-mab-je33-30-278202415212488.jpg "Western Blot: ELF3/ESE-1 Antibody (JE33-30) [NBP3-32320] - Western blot analysis of ELF3/ESE-1 on different lysates with Rabbit anti-ELF3/ESE-1 antibody (NBP3-32320) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 ug/Lane) Lane 2: SK-Br-3 cell lysate (10 ug/Lane) Lane 3: A431 cell lysate (10 ug/Lane) Lane 4: Mouse lung tissue lysate (20 ug/Lane) Lane 5: Rat kidney tissue lysate (20 ug/Lane) Predicted band size: 41.5 kDa Observed band size: 41.5 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32320) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: ELF3/ESE-1 Antibody (JE33-30) [NBP3-32320] - Flow cytometric analysis of A431 cells labeling ELF3/ESE-1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32320, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32320_rabbit-elf3-ese-1-mab-je33-30-392024884669.jpg)
![Western Blot: ELF3/ESE-1 Antibody (SR1813) [NBP3-22292] - Western blot analysis using NBP3-22292 in (1) A431 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22292_rabbit-elf3-ese-1-mab-sr1813-57202316494294.jpg "Western Blot: ELF3/ESE-1 Antibody (SR1813) [NBP3-22292] - Western blot analysis using NBP3-22292 in (1) A431 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.")
![SDS-Page: Recombinant Human ELF3/ESE-1 Protein [H00001999-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001999-P01-1.jpg "SDS-Page: Recombinant Human ELF3/ESE-1 Protein [H00001999-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human ELF3/ESE-1 Protein [H00001999-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001999-Q01-1.jpg "SDS-Page: Recombinant Human ELF3/ESE-1 Protein [H00001999-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: ESE1 Overexpression Lysate (Adult Normal) [NBP2-11404] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ESE1.](http://images.novusbio.com/fullsize/ESE1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11404-img0001.jpg "Western Blot: ESE1 Overexpression Lysate (Adult Normal) [NBP2-11404] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ESE1.")