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![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Analysis in human lung and liver tissues. Corresponding CAPG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Analysis in human lung and liver tissues. Corresponding CAPG RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lung, lymph node, skeletal muscle and small intestine using Anti-CAPG antibody (A) NBP1-90214 shows similar protein distribution across tissues to independent antibody NBP1-90215 (B).](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0021.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CapG Antibody [NBP1-90214] - Analysis using Anti-CAPG antibody NBP1-90214 (A) shows similar pattern to independent antibody NBP1-90213 (B).](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP1-90214-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: CapG Antibody [NBP1-90214] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90214-img0008.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0016.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0017.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0018.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0019.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90214] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in cells in lamina propria.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90214-img0020.jpg)
![Western Blot: CapG Antibody [NBP1-90215] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP1-90215-img0006.jpg "Western Blot: CapG Antibody [NBP1-90215] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90215] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in cells in lamina propria.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90215-img0020.jpg)
![Western Blot: CapG Antibody [NBP1-90215] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP1-90215-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90215] - Analysis in human lung and liver tissues. Corresponding CAPG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90215-img0015.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90215] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90215-img0016.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90215] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90215-img0017.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90215] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90215-img0018.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90215] - Staining of human skeletal muscle shows negative to very weak positivity in myocytes.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90215-img0019.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90215] - Staining of human kidney, placenta, skeletal muscle and small intestine using Anti-CD2AP antibody (A) NBP1-90215 shows similar protein distribution across tissues to independent antibody NBP1-90214 (B).](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90215-img0021.jpg)
![Western Blot: CapG Antibody [NBP1-90213] - Analysis using Anti-CAPG antibody NBP1-90213 (A) shows similar pattern to independent antibody NBP1-90214 (B).](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP1-90213-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CapG Antibody [NBP1-90213] - Analysis using Anti-CAPG antibody NBP1-90213 (A) shows similar pattern to independent antibody NBP1-90214 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90213] - Staining of human lymph node.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90213-img0010.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90213] - Staining of human lung shows strong cytoplasmic and nuclear positivity in macrophages.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90213-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90213] - Staining of human kidney, lung, lymph node and skeletal muscle using Anti-CAPG antibody NBP1-90213 (A) shows similar protein distribution across tissues to independent antibody NBP1-90215 (B).](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90213-img0005.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90213] - Staining of human lung.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90213-img0007.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90213] - Staining of human kidney.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90213-img0008.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP1-90213] - Staining of human skeletal muscle.](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP1-90213-img0009.jpg)
![Western Blot: CapG Antibody [NBP2-92955] - Analysis of extracts of various cell lines, using CapG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP2-92955-img0005.jpg "Western Blot: CapG Antibody [NBP2-92955] - Analysis of extracts of various cell lines, using CapG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry-Paraffin: CapG Antibody [NBP2-92955] - Paraffin-embedded human lung cancer using CapG .](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP2-92955-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CapG Antibody [NBP2-92955] - Analysis of extracts from normal (control) and CAPG knockout (KO) HeLa cells, using CapG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP2-92955-img0003.jpg)
![Western Blot: CapG Antibody [NBP2-92955] - Analysis of extracts of various cell lines, using CapG at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP2-92955-img0004.jpg)
![Immunohistochemistry-Paraffin: CapG Antibody [NBP2-92955] - Paraffin-embedded human liver cancer using CapG .](http://images.novusbio.com/fullsize/CapG-Antibody-Immunohistochemistry-Paraffin-NBP2-92955-img0001.jpg)
![Immunohistochemistry-Paraffin: Actin Regulatory Protein Antibody [NBP2-15259] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Actin-Regulatory-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-15259-img0002.jpg "Immunohistochemistry-Paraffin: Actin Regulatory Protein Antibody [NBP2-15259] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.")
![Western Blot: Actin Regulatory Protein Antibody [NBP2-15259] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/Actin-Regulatory-Protein-Antibody-Western-Blot-NBP2-15259-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CapG Antibody [NBP2-15259] - CapG antibody [N3C3] detects CapG protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CapG protein stained by CapG antibody [N3C3] (NBP2-15259) diluted at 1:400.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15259_rabbit-polyclonal-capg-antibody-910202420104435.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CapG Antibody [NBP2-15259] - Wild-type (WT) and CAPG knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CAPG antibody [N3C3] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15259_rabbit-polyclonal-capg-antibody-1610202419414516.jpg)
![Western Blot: CapG Antibody (1F1) [H00000822-M01] - CAPG monoclonal antibody (M01), clone 1F1 Analysis of CAPG expression in HL-60.](http://images.novusbio.com/fullsize/CapG-Antibody-1F1-Western-Blot-H00000822-M01-img0010.jpg "Western Blot: CapG Antibody (1F1) [H00000822-M01] - CAPG monoclonal antibody (M01), clone 1F1 Analysis of CAPG expression in HL-60.")
![Immunocytochemistry/Immunofluorescence: CapG Antibody (1F1) [H00000822-M01] - Analysis of monoclonal antibody to CAPG on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/CapG-Antibody-1F1-Immunocytochemistry-Immunofluorescence-H00000822-M01-img0008.jpg)
![Sandwich ELISA: CapG Antibody (1F1) [H00000822-M01] - Detection limit for recombinant GST tagged CAPG is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CapG-Antibody-1F1-Sandwich-ELISA-H00000822-M01-img0009.jpg)
![Western Blot: CapG Antibody (1D10) [NBP2-59450] - Lane 1: HeLa cell lysates, Lane 2: NIH3T3 cell lysates, Lane 3: Mouse spleen tissue lysates, Lane 4: THP-1 cell lysates, Lane 5: Raw 264.7 cell lysates](http://images.novusbio.com/fullsize/CapG-Antibody-1D10-Western-Blot-NBP2-59450-img0001.jpg "Western Blot: CapG Antibody (1D10) [NBP2-59450] - Lane 1: HeLa cell lysates, Lane 2: NIH3T3 cell lysates, Lane 3: Mouse spleen tissue lysates, Lane 4: THP-1 cell lysates, Lane 5: Raw 264.7 cell lysates")
![Immunocytochemistry/Immunofluorescence: CapG Antibody (1D10) [NBP2-59450] - Analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/CapG-Antibody-1D10-Immunocytochemistry-Immunofluorescence-NBP2-59450-img0002.jpg)
![Western Blot: CapG Antibody (5V9G1) [NBP3-16408] - Western blot analysis of extracts of various cell lines, using CapG Rabbit mAb (NBP3-16408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CapG-Antibody-5V9G1-Western-Blot-NBP3-16408-img0001.jpg "Western Blot: CapG Antibody (5V9G1) [NBP3-16408] - Western blot analysis of extracts of various cell lines, using CapG Rabbit mAb (NBP3-16408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: CapG Antibody (5V9G1) [NBP3-16408] - Western blot analysis of extracts of various cell lines, using CapG Rabbit mAb (NBP3-16408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CapG-Antibody-5V9G1-Western-Blot-NBP3-16408-img0002.jpg)
![Western Blot: CapG Antibody (6D6) [H00000822-M02] - CAPG monoclonal antibody (M02), clone 6D6 Analysis of CAPG expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CapG-Antibody-6D6-Western-Blot-H00000822-M02-img0007.jpg "Western Blot: CapG Antibody (6D6) [H00000822-M02] - CAPG monoclonal antibody (M02), clone 6D6 Analysis of CAPG expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: CapG Antibody (6D6) [H00000822-M02] - Analysis of monoclonal antibody to CAPG on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/CapG-Antibody-6D6-Immunocytochemistry-Immunofluorescence-H00000822-M02-img0006.jpg)
![Western Blot: CapG Antibody [NBP3-00258] - Anti-CapG rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/CapG-Antibody-Western-Blot-NBP3-00258-img0004.jpg "Western Blot: CapG Antibody [NBP3-00258] - Anti-CapG rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa")
![Immunoprecipitation: CapG Antibody [NBP3-00258] - CapG was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-CapG rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CapG rabbit polyclonal antibody, at 1:330 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/CapG-Antibody-Immunoprecipitation-NBP3-00258-img0003.jpg)
![Western Blot: CapG Antibody (SR1632) [NBP3-22152] - Western blot analysis using NBP3-22152 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22152_rabbit-capg-mab-sr1632-672023953370.jpg "Western Blot: CapG Antibody (SR1632) [NBP3-22152] - Western blot analysis using NBP3-22152 in HeLa cell lysate.")