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![Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Western-Blot-NB100-360-img0003.jpg "Western Blot: ATP7b Antibody [NB100-360] - ATP7b detected in 20 ug of mouse brain membrane fraction.")
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-360] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ATP7b [NB100-360] at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NB100-360-img0005.jpg)
![Immunohistochemistry: ATP7b Antibody [NB100-360] - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-NB100-360-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-361] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATP7b Antibody NB100-361 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NB100-361-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NB100-361] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATP7b Antibody NB100-361 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ATP7b Antibody [NB100-361] - Effects & mechanism of action of CASIN on melphalan-resistant MM cells. (A) CASIN reduces Cdc42 activity (Cdc42-GTP) but not Rac1 activity (Rac1-GTP). Melphalan-sensitive MM cells (S) were treated with Vehicle or CASIN (5 μM) for 8 h. Cdc42 & Rac1 activities were measured using pull-down assay. (B) CASIN (5 μM) inhibits proliferation of both S & melphalan-resistant (LR5) MM cells. **P < 0.01 vs. S-Vehicle; ##P < 0.01 vs. LR5-Vehicle. (C) CASIN (5 μM) preferentially causes apoptosis of melphalan-resistant MM cells. S & LR5 cells were treated with or without CASIN or melphalan (Mel, 25 μM) or both for 2 days. Annexin V+ cells were analyzed using flow cytometry. **P < 0.01. (D–F) Mechanism of action of CASIN on melphalan-resistant MM cells. (D) Cdc42 activity & expression are increased in LR5 cells compared to that in S cells. Cdc42 activity was measured using pull-down assay. beta -Actin was used as loading control. Vertical lines indicate the gel lanes being switched in position from the original blots. (E) CASIN (5 μM) decreases melphalan-induced FANCD2 mono-ubiquitination (Ub-FANCD2) in LR5 but not in S cells. S & LR5 cells were treated with or without CASIN, Mel (25 μM), or both for 16 h. FANCD2 was detected using western blotting. beta -Actin was used as loading control. (F) CASIN sensitizes melphalan-resistant but not -sensitive MM cells to melphalan-induced DNA damage. gamma H2A histone family member X-positive ( gamma H2AX+) cells were detected using flow cytometry. **P < 0.01. Error bars represent means ± SD of triplicates & data are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31632904), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-361_rabbit-polyclonal-atp7b-antibody-310202415535181.jpg)
![Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Western-Blot-NBP2-59375-img0005.jpg "Western Blot: ATP7b Antibody (S62-29) [NBP2-59375] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (S62-29) [NBP2-59375] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29 (NBP2-59375). Tissue: NIH 3T3 (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody (NBP2-59375) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Copper Transporting ATPase 2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/ATP7b-Antibody-S62-29-Immunocytochemistry-Immunofluorescence-NBP2-59375-img0007.jpg)
![Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-262202414471517.jpg "Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.")
![Immunohistochemistry: ATP7b Antibody (36D12) [NBP3-26488] - Image of ATP7b Antibody (36D12) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.19% DAB.](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-262202415253317.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (36D12) [NBP3-26488] - Staining of HepG2 with ATP7b Antibody (36D12) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-28220241323633.jpg)
![Flow Cytometry: ATP7b Antibody (36D12) [NBP3-26488] - Overlay Peak curve showing HepG2 cells stained with ATP7b Antibody (36D12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26488_rabbit-atp7b-mab-36d12-282202414183628.jpg)
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0004.jpg "Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Liver shows strong granular cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0003.jpg)
![Immunohistochemistry-Paraffin: ATP7b Antibody [NBP2-48523] - Staining of human Testis shows moderate granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunohistochemistry-Paraffin-NBP2-48523-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody (9H8) [NBP3-26489] - Staining of Hela cell with ATP7b Antibody (9H8) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26489_rabbit-atp7b-mab-9h8-28220241312249.jpg "Immunocytochemistry/Immunofluorescence: ATP7b Antibody (9H8) [NBP3-26489] - Staining of Hela cell with ATP7b Antibody (9H8) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: ATP7b Antibody (9H8) [NBP3-26489] - Overlay Peak curve showing Hela cells stained with ATP7b Antibody (9H8) (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26489_rabbit-atp7b-mab-9h8-282202414312774.jpg)
![Western Blot: ATP7b Antibody (3E10) [H00000540-M01] - ATP7B monoclonal antibody (M01), clone 3E10. Analysis of ATP7B expression in human colon.](http://images.novusbio.com/fullsize/ATP7b-Antibody-3E10-Western-Blot-H00000540-M01-img0003.jpg "Western Blot: ATP7b Antibody (3E10) [H00000540-M01] - ATP7B monoclonal antibody (M01), clone 3E10. Analysis of ATP7B expression in human colon.")
![ELISA: ATP7b Antibody (3E10) [H00000540-M01] - ATP7b Antibody (3E10) [H00000540-M01] - Detection limit for recombinant GST tagged ATP7B is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATP7b-Antibody-3E10-ELISA-H00000540-M01-img0002.jpg)
![SDS-Page: Recombinant Human ATP7b Protein [H00000540-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-ATP7b-Protein-SDS-Page-H00000540-Q01-img0002.jpg "SDS-Page: Recombinant Human ATP7b Protein [H00000540-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NBP2-58923] - Staining of human cell line CACO-2 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/ATP7b-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58923-img0001.jpg "Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NBP2-58923] - Staining of human cell line CACO-2 shows localization to the Golgi apparatus.")