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![Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Western-Blot-NBP1-39474-img0003.jpg "Western Blot: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Adenosine A2a R (7F6-G5-A2) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0005.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Staining of human brain (putamen), antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0002.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-87 MG cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Adenosine A2a R (7F6-G5-A2), NBP1-39474, at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunocytochemistry-Immunofluorescence-NBP1-39474-img0004.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:100 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Analysis of a FFPE tissue section of mouse brain using Adenosine A2a R antibody (clone 7F6-G5-A2) at 1:400 dilution. The antibody generated nice membranous / punctate staining of Adenosine A2a receptors.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Immunohistochemistry-Paraffin-NBP1-39474-img0008.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on hPBMCs with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control NBP2-14864. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0009.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control NB600-986PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0011.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0012.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin (PE).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0013.jpg)
![Flow (Intracellular): Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin (APC).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Intracellular-NBP1-39474-img0014.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on SH-SY5Y with Adenosine A2a R Antibody (7F6-G5-A2) NBP1-39474 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B).](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0015.jpg)
![Flow Cytometry: Adenosine A2aR Antibody (7F6-G5-A2) [NBP1-39474] - An intracellular stain was performed on U-937 cells with Adenosine A2a R (7F6-G5-A2) antibody NBP1-39474F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-7F6-G5-A2-Flow-Cytometry-NBP1-39474-img0016.jpg)
![Western Blot: Adenosine A2aR Antibody [NB300-597] - Analysis of 25 ug of human placenta (lane 1), HepG2 (lane 2), Hela (lane 3) and mouse liver (lane 4) cell lysates.](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Western-Blot-NB300-597-img0011.jpg "Western Blot: Adenosine A2aR Antibody [NB300-597] - Analysis of 25 ug of human placenta (lane 1), HepG2 (lane 2), Hela (lane 3) and mouse liver (lane 4) cell lysates.")
![Simple Western: Adenosine A2aR Antibody [NB300-597] - Simple Western lane view shows a specific band for Adenosine A2a R in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Simple-Western-NB300-597-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Immunohistochemistry-Paraffin-NB300-597-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody [NB300-597] - Analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-597-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody [NB300-597] - Mixed cortical rat neurons culture (21 DIV) stained for A2A labelled with Alexa647. The staining visualised the astrocytes in culture. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Immunocytochemistry-NB300-597-img0012.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Staining in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Immunohistochemistry-Paraffin-NB300-597-img0008.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Immunohistochemistry-Paraffin-NB300-597-img0009.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Analysis in human bone marrow and skeletal muscle tissues using NBP2-62698 antibody. Corresponding ADORA2A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Analysis in human bone marrow and skeletal muscle tissues using NBP2-62698 antibody. Corresponding ADORA2A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0009.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human caudate shows strong membranous positivity.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0007.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0008.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0010.jpg)
![Western Blot: Adenosine A2a R Antibody [H00000135-B01P] - Analysis of ADORA2A expression in HeLa.](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Western-Blot-H00000135-B01P-img0002.jpg "Western Blot: Adenosine A2a R Antibody [H00000135-B01P] - Analysis of ADORA2A expression in HeLa.")
![Flow Cytometry: Adenosine A2a R Antibody [H00000135-B01P] - Analysis of negative control 293 cells (Black) and ADORA2A expressing 293 cells (Green) using ADORA2A purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Flow-Cytometry-H00000135-B01P-img0001.jpg)
![Western Blot: Adenosine A2a R Antibody [H00000135-B01P] - Analysis of ADORA2A expression in transfected 293T cell line by ADORA2A polyclonal antibody. Lane 1: ADORA2A transfected lysate(45.43 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Adenosine-A2a-R-Antibody-Western-Blot-H00000135-B01P-img0003.jpg)
![SDS-Page: Recombinant Human Adenosine A2a R Protein [H00000135-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Adenosine-A2a-R-Protein-SDS-Page-H00000135-Q01-img0001.jpg "SDS-Page: Recombinant Human Adenosine A2a R Protein [H00000135-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human Adenosine A2a R Protein [H00000135-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Adenosine-A2a-R-Protein-SDS-Page-H00000135-P02-img0001.jpg "SDS-Page: Recombinant Human Adenosine A2a R Protein [H00000135-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Human Adenosine A2aR ELISA Kit (Colorimetric) [NBP2-66650] - Samples were spiked with high concentrations of Human Adenosine A2aR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66650_human-adenosine-a2ar-elisa-kit-colorimetric-132202416232327.jpg "ELISA: Human Adenosine A2aR ELISA Kit (Colorimetric) [NBP2-66650] - Samples were spiked with high concentrations of Human Adenosine A2aR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Adenosine A2aR ELISA Kit (Colorimetric) [NBP2-66650] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66650_human-adenosine-a2ar-elisa-kit-colorimetric-4320249124820.jpg)
![Western Blot: Adenosine A2aR Overexpression Lysate [NBP2-04171] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenosine A2a Receptor.](http://images.novusbio.com/fullsize/Adenosine-A2a-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04171-img0001.jpg "Western Blot: Adenosine A2aR Overexpression Lysate [NBP2-04171] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenosine A2a Receptor.")
![Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric [NBP3-09003] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Ig2838 (NBP3-09003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing granular cytoplasmic staining, possibly of protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09003, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Ig2838-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09003-img0001.jpg "Immunocytochemistry/Immunofluorescence: Adenosine A2aR Antibody (Ig2838) - Chimeric [NBP3-09003] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of Ig2838 (NBP3-09003) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing granular cytoplasmic staining, possibly of protein in trafficking vesicles. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09003, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor(R) 488 secondary antibody.")