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![Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OPA1-Antibody-Simple-Western-NB110-55290-img0007.jpg "Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: OPA1 Antibody [NB110-55290] - Depletion of Oma1 in fish affects development. Immunoblot of protein extracts from injected fish (yolk removed) at 24, 48 and 72 hpf. Expression of Oma1, Opa1 isoforms (a-f) and Cox4 (mitochondrial abundance marker) was analyzed with respective antibodies. Actin and Ponceau S staining were loading controls. Source data (full-length blots) are available online in Supplementary information. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep13989), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OPA1-Antibody-Western-Blot-NB110-55290-img0010.jpg)
![Western Blot: OPA1 Antibody [NB110-55290] - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-OPA1 in 1% blocking buffer. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). The protein ladder was detected with an anti-mouse HRP secondary antibody and OPA1 with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/OPA1-Antibody-Western-Blot-NB110-55290-img0009.jpg)
![Immunohistochemistry: OPA1 Antibody [NB110-55290] - Staining in prostatic smooth muscle and glandular epithelium. Human Prostate 40X magnification.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-NB110-55290-img0005.jpg)
![Western Blot: OPA1 Antibody [NB110-55290] - Detection of OPA1 protein in post-nuclear extracts of mouse embryonic fibroblasts.](http://images.novusbio.com/fullsize/OPA1-Antibody-Western-Blot-NB110-55290-img0006.jpg)
![Western Blot: OPA1 Antibody [NB110-55290] - Analysis of OPA1 in porcine retinal pigment epithelium lysate using anti-OPA1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/OPA1-Antibody-Western-Blot-NB110-55290-img0008.jpg)
![Western Blot: OPA1 Antibody - BSA Free [NB110-55290] - High content screening of the preadipocytes & beige cells, & detection of mitochondrial fission- & fusion-related proteins: (A) Representative high content screening images showing mitochondrial morphology in TG2+/+ & TG2−/− preadipocytes, scale bars represent 50 μm; (B) fractions of fragmented & tubular mitochondrial morphology in preadipocytes (%); (C) quantitative analysis of mitochondrial morphology in preadipocytes; (D) representative high content screening images showing mitochondrial morphology in TG2+/+ & TG2−/− beige cells; (E) fractions of fragmented & tubular mitochondrial morphology in beige cells (%); (F) quantitative analysis of mitochondrial morphology in beige cells, DAPI staining was used to determine the number of nuclei, the mitochondria were stained with Mito Tracker Deep Red either in the absence or presence of 10 μM antimycin A, Texas Red-X phalloidin was used to stain actin filaments for the detection of cell shapes (n = 3); (G–J) representative Western blot analyses & quantitative analyses of the mitochondrial fusion proteins (MFN2, OPA1) & mitochondrial fission proteins (DRP1, MFF) in preadipocytes & differentiated beige cells. beta -ACTIN was used as a loading control. Columns represent the mean values ± SD. Statistical analyses were performed using Student’s t-test. n = 5. * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563567), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-55290_rabbit-polyclonal-opa1-antibody-31020241624918.jpg)
![Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Western-Blot-NBP2-59770-img0001.jpg "Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with OPA1 Antibody [1284B] conjugated to Biotin (NBP2-59770B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0022.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) [NBP2-59770] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This OPA1 antibody generated a diffused cytoplasmic staining of OPA1 protein in the tubular epithelial cells, the spermatids/spermatocytes and Leydig's cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunohistochemistry-Paraffin-NBP2-59770-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0002.jpg)
![Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Western-Blot-NBP1-71656-img0009.jpg "Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunocytochemistry-Immunofluorescence-NBP1-71656-img0007.jpg)
![Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunohistochemistry-NBP1-71656-img0008.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Simple-Western-NBP1-71656-img0010.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-BSA-Free-Simple-Western-NBP1-71656-img0011.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Analysis in human cerebral cortex and pancreas tissues . Corresponding OPA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Analysis in human cerebral cortex and pancreas tissues . Corresponding OPA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: OPA1 Antibody [NBP2-34206] - Staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34206-img0006.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0007.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0009.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-34206] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-34206-img0010.jpg)
![Western Blot: OPA1 Antibody [NBP2-48669] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/OPA1-Antibody-Western-Blot-NBP2-48669-img0002.jpg "Western Blot: OPA1 Antibody [NBP2-48669] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: OPA1 Antibody [NBP2-48669] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/OPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-48669-img0003.jpg)
![Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05725] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.](http://images.novusbio.com/fullsize/OPA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05725-img0001.jpg "Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05725] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.")
![Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05723] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.](http://images.novusbio.com/fullsize/OPA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05723-img0001.jpg "Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05723] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.")
![Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-07150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.](http://images.novusbio.com/fullsize/OPA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07150-img0001.jpg "Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-07150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.")
![Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05727] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.](http://images.novusbio.com/fullsize/OPA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05727-img0001.jpg "Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05727] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.")
![Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05726] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.](http://images.novusbio.com/fullsize/OPA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05726-img0001.jpg "Western Blot: OPA1 Overexpression Lysate (Adult Normal) [NBP2-05726] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OPA1.")