| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56509SS | |||
| Azide and BSA Free | NBP2-80788 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56513SS | |||
| Azide Free | NBP2-33214 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7155-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4535-SP | |||
![Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Representative western blot of IKKB (E) in monocytes with quantification on the right. All data are representative of 5 separate experiments. Unpaired student t-test. ***p < 0.001. Image collected and cropped by CiteAb from the following publication (doi.org/10.3389/fimmu.2020.01478) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Western-Blot-NB100-56509-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Representative western blot of IKKB (E) in monocytes with quantification on the right. All data are representative of 5 separate experiments. Unpaired student t-test. ***p < 0.001. Image collected and cropped by CiteAb from the following publication (doi.org/10.3389/fimmu.2020.01478) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of IKK beta Antibody in HeLa cells. Antibody dilution 5 ug/ml. Image from verified customer review.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Immunocytochemistry-Immunofluorescence-NB100-56509-img0016.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of IKK beta clone 10AG2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Immunohistochemistry-Paraffin-NB100-56509-img0014.jpg)
![Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized wi](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Flow-Cytometry-NB100-56509-img0012.jpg)
![Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Western-Blot-NB100-56509-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (10AG2) [NB100-56509] - U-87 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-IKK beta Antibody (10AG2) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Immunocytochemistry-Immunofluorescence-NB100-56509-img0013.jpg)
![Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green histogram represents the isotype control , red represents the IKKbeta antibody.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Flow-Cytometry-NB100-56509-img0010.jpg)
![Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Flow-Cytometry-NB100-56509-img0011.jpg)
![Simple Western: IKK beta Antibody (10AG2) [NB100-56509] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10AG2-Simple-Western-NB100-56509-img0008.jpg)
![Western Blot: IKK beta Antibody (10AG2) - BSA Free [NB100-56509] - TRAM is required for the pro-inflammatory polarization of macrophages by SLD-LPS. (A,B) Flow cytometry analysis of Ly6C (A) or CD200R (B) expression in WT or TRAM−/− live-cell-gated macrophages with geometric MFI quantification on right. Pink = PBS, Blue = 100 pg/ml LPS. (C–E) Representative Western blots of IKK-beta (C), p-p65 (D), & total NF-kappa B p65 (E) expression in WT & TRAM−/− monocytes treated with either PBS or 100 pg/mL LPS for 5 days. Quantification is depicted on the right. (F) Ratio of p-p65/total p65 (p-p65 levels from (D) divided by corresponding total p65 levels). All data are representative of at least 3 separate experiments (n = 3) for (A,B) & n = 6 for (C–F). ANOVA with Tukey's multiple comparisons test (A,B); unpaired student t-test (C–F) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s, non-significant; MFI, Mean fluorescence intensity. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32765513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56509_mouse-monoclonal-ikk-beta-antibody-10ag2-31020241554520.jpg)
![Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Western-Blot-NB100-56513-img0005.jpg "Western Blot: IKK beta Antibody (10A9B6) [NB100-56513] - IKK beta in Daudi cell lysate using IKK beta antibody at 5 ug/ml.")
![Immunohistochemistry-Frozen: IKK beta Antibody (10A9B6) [NB100-56513] - Mouse skin tissue section stained with IKK beta antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Immunohistochemistry-Frozen-NB100-56513-img0013.jpg)
![Flow (Intracellular): IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control , red represents the IKK antibody. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Flow-Intracellular-NB100-56513-img0011.jpg)
![Simple Western: IKK beta Antibody (10A9B6) [NB100-56513] - Analysis using Azide Free version of NB100-56513. Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Simple-Western-NB100-56513-img0010.jpg)
![Immunoprecipitation: IKK beta Antibody (10A9B6) [NB100-56513] - Antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-10A9B6-Western-Blot-NB100-56513-img0012.jpg)
![Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NB600-477-img0006.jpg "Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.")
![Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Paraffin-embedded mouse brain sections (IKK beta KO or IKK beta WT) were stained with IKK beta (green). Note: during staining, remember to add 0.1-0.3% Triton-100 in the antibody incubation buffer. See PMID 25253847 for further details regarding the experiemental conditions. Image from verified customer review.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NB600-477-img0003.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Human placenta tissue.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NB600-477-img0004.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0008.jpg "Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody [NBP1-32258] - analysis of A431, using IKK beta antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32258-img0002.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NBP1-32258] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-32258-img0003.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0004.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0005.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of DDDDK-human IKBKB-transfected 293T cells. 7.5 % SDS-PAGE. IKK beta antibody dilution: 1:5000](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0006.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0007.jpg)
![Western Blot: IKK beta Antibody [NBP1-87699] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-IKBKB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-87699-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: IKK beta Antibody [NBP1-87699] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-IKBKB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: IKK beta Antibody [NBP1-87699] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87699-img0008.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NBP1-87699] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-87699-img0009.jpg)
![Western Blot: IKK beta Antibody (SN63-02) [NBP2-67659] - Western blot analysis of IKK beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-](http://images.novusbio.com/fullsize/IKK-beta-Antibody-SN63-02-Western-Blot-NBP2-67659-img0005.jpg "Western Blot: IKK beta Antibody (SN63-02) [NBP2-67659] - Western blot analysis of IKK beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-")
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659] - Staining IKK alpha+IKK beta in B-6F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-SN63-02-Immunocytochemistry-Immunofluorescence-NBP2-67659-img0004.jpg)
![Western Blot: IKK beta Antibody (SN63-02) [NBP2-67659] - Analysis of IKK alpha+IKK beta on different lysates using anti-IKK alpha+IKK beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Daudi Lane 3: A431](http://images.novusbio.com/fullsize/IKK-beta-Antibody-SN63-02-Western-Blot-NBP2-67659-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659] - Staining IKK alpha+IKK beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-SN63-02-Immunocytochemistry-Immunofluorescence-NBP2-67659-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659] - Staining IKK alpha+IKK beta in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-SN63-02-Immunocytochemistry-Immunofluorescence-NBP2-67659-img0002.jpg)
![Western Blot: IKK beta Antibody (7G8J8) [NBP3-15810] - Western blot analysis of extracts of various cell lines, using IKK beta antibody (NBP3-15810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-7G8J8-Western-Blot-NBP3-15810-img0004.jpg "Western Blot: IKK beta Antibody (7G8J8) [NBP3-15810] - Western blot analysis of extracts of various cell lines, using IKK beta antibody (NBP3-15810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (7G8J8) [NBP3-15810] - Immunofluorescence analysis of PC-12 cells using IKK beta Rabbit mAb (NBP3-15810) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-7G8J8-Immunocytochemistry-Immunofluorescence-NBP3-15810-img0003.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody (7G8J8) [NBP3-15810] - Immunohistochemistry of paraffin-embedded human lung cancer using IKK beta antibody (NBP3-15810) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-7G8J8-Immunohistochemistry-Paraffin-NBP3-15810-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (7G8J8) [NBP3-15810] - Immunofluorescence analysis of NIH/3T3 cells using IKK beta Rabbit mAb (NBP3-15810) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-7G8J8-Immunocytochemistry-Immunofluorescence-NBP3-15810-img0002.jpg)
![Immunohistochemistry: IKK beta Antibody (3E1) [NBP3-26206] - Image of IKK beta Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-262202415475613.jpg "Immunohistochemistry: IKK beta Antibody (3E1) [NBP3-26206] - Image of IKK beta Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: IKK beta Antibody (3E1) [NBP3-26206] - Positive Western Blot detected in: K562 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate. All lanes: IKK beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87 kDa Observed band size: 87 kDa](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-28220241140932.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (3E1) [NBP3-26206] - Staining of Hela Cells with IKK beta Antibody (3E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-2822024132367.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (3C12) [H00003551-M08] - Analysis of monoclonal antibody to IKBKB on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/IKK-beta-Antibody-3C12-Immunocytochemistry-Immunofluorescence-H00003551-M08-img0003.jpg "Immunocytochemistry/Immunofluorescence: IKK beta Antibody (3C12) [H00003551-M08] - Analysis of monoclonal antibody to IKBKB on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: IKK beta Antibody (3C12) [H00003551-M08] - Detection limit for recombinant GST tagged IKBKB is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-3C12-Sandwich-ELISA-H00003551-M08-img0004.jpg)
![Western Blot: IKK beta Antibody (S01-9H6) [NBP3-19661] - Western blot detection of IKK beta in 3T3 cell lysates using NBP3-19661 (1:1000 diluted). Predicted band size: 87kDa. Observed band size: 87kDa.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-S01-9H6-Western-Blot-NBP3-19661-img0001.jpg "Western Blot: IKK beta Antibody (S01-9H6) [NBP3-19661] - Western blot detection of IKK beta in 3T3 cell lysates using NBP3-19661 (1:1000 diluted). Predicted band size: 87kDa. Observed band size: 87kDa.")