![Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] - Image of HIST1H3A Antibody (9G8) diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26231_mouse-hist1h3a-mab-9g8-27220248573318.jpg "Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] - Image of HIST1H3A Antibody (9G8) diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 28H10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | Recombinant Human HIST1H3A protein [UniProt P68431] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H3C1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol, 0.01M PBS, PH 7.4 |
| Preservative | 0.03% Proclin 300 |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | H3C1 |
![Western Blot: HIST1H3A Antibody (9G8) [NBP3-26231] - Positive Western Blot detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: HIST1H3A Antibody at 1: 1000 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/nbp3-26231_mouse-hist1h3a-mab-9g8-28220241140916.jpg "Western Blot: HIST1H3A Antibody (9G8) [NBP3-26231] - Positive Western Blot detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: HIST1H3A Antibody at 1: 1000 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 16 kDa Observed band size: 16 kDa")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (9G8) [NBP3-26231] - Staining of HepG2 cell with HIST1H3A Antibody (9G8) at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26231_mouse-hist1h3a-mab-9g8-28220241247511.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (9G8) [NBP3-26231] - Staining of HepG2 cell with HIST1H3A Antibody (9G8) at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).")
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](https://images.novusbio.com/images/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")