| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74145 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34101-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20145-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03470-20ul | |||
![Western Blot: SETD7/9 Antibody [NB100-56664] - Whole cell extract from MCF7 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/SETD7-9-Antibody-Western-Blot-NB100-56664-img0005.jpg "Western Blot: SETD7/9 Antibody [NB100-56664] - Whole cell extract from MCF7 cells. Image from verified customer review.")
![Immunohistochemistry-Paraffin: SETD7/9 Antibody [NB100-56664] - Analysis of human small instestine using SETD7 antibody at 1:100.](http://images.novusbio.com/fullsize/SETD7-9-Antibody-Immunohistochemistry-Paraffin-NB100-56664-img0003.jpg)
![Western Blot: SETD7/9 Antibody [NB100-56664] - Analysis of SETD7 in A) MCF7 and B) HeLa lysate using SETD7 antibody at 1:500.](http://images.novusbio.com/fullsize/SETD7-9-Antibody-Western-Blot-NB100-56664-img0004.jpg)
![Western Blot: SETD7 Antibody (2D10) [NBP2-01066] Analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody.](http://images.novusbio.com/fullsize/SETD7-Antibody-2D10-Western-Blot-NBP2-01066-img0006.jpg "Western Blot: SETD7 Antibody (2D10) [NBP2-01066] Analysis of extracts (35ug) from 9 different cell lines by using anti-SETD7 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SETD7 Antibody (2D10) [NBP2-01066] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SETD7.](http://images.novusbio.com/fullsize/SETD7-Antibody-2D10-Immunocytochemistry-Immunofluorescence-NBP2-01066-img0005.jpg)
![Immunohistochemistry-Paraffin: SETD7 Antibody (2D10) [NBP2-01066] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SETD7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SETD7-Antibody-2D10-Immunohistochemistry-Paraffin-NBP2-01066-img0004.jpg)
![Flow Cytometry: SETD7 Antibody (2D10) [NBP2-01066] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SETD7 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SETD7-Antibody-2D10-Flow-Cytometry-NBP2-01066-img0001.jpg)
![Western Blot: SETD7 Antibody (2D10) [NBP2-01066] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SETD7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD7.](http://images.novusbio.com/fullsize/SETD7-Antibody-2D10-Western-Blot-NBP2-01066-img0002.jpg)
![Immunohistochemistry-Paraffin: SETD7 Antibody (2D10) [NBP2-01066] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SETD7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SETD7-Antibody-2D10-Immunohistochemistry-Paraffin-NBP2-01066-img0003.jpg)
![Western Blot: SETD7 Antibody (5B4) [H00080854-M01] - SET7 monoclonal antibody (M01), clone 5B4 Analysis of SET7 expression in Jurkat.](http://images.novusbio.com/fullsize/SETD7-Antibody-5B4-Western-Blot-H00080854-M01-img0008.jpg "Western Blot: SETD7 Antibody (5B4) [H00080854-M01] - SET7 monoclonal antibody (M01), clone 5B4 Analysis of SET7 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: SETD7 Antibody (5B4) [H00080854-M01] - Analysis of monoclonal antibody to SET7 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SETD7-Antibody-5B4-Immunocytochemistry-Immunofluorescence-H00080854-M01-img0004.jpg)
![Immunohistochemistry-Paraffin: SETD7 Antibody (5B4) [H00080854-M01] - Analysis of monoclonal antibody to SETD7 on formalin-fixed paraffin-embedded human colon. Antibody concentration 0.5 ug/ml.](http://images.novusbio.com/fullsize/SETD7-Antibody-5B4-Immunohistochemistry-Paraffin-H00080854-M01-img0006.jpg)
![Sandwich ELISA: SETD7 Antibody (5B4) [H00080854-M01] - Detection limit for recombinant GST tagged SETD7 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SETD7-Antibody-5B4-Sandwich-ELISA-H00080854-M01-img0007.jpg)
![Western Blot: SETD7 Antibody [NBP2-34101] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: U-87 MG](http://images.novusbio.com/fullsize/SETD7-Antibody-Western-Blot-NBP2-34101-img0004.jpg "Western Blot: SETD7 Antibody [NBP2-34101] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: U-87 MG")
![Immunocytochemistry/Immunofluorescence: SETD7 Antibody [NBP2-34101] - Staining of human cell line MCF7 shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SETD7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34101-img0008.jpg)
![Immunohistochemistry-Paraffin: SETD7 Antibody [NBP2-34101] - Staining of human vagina shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SETD7-Antibody-Immunohistochemistry-Paraffin-NBP2-34101-img0007.jpg)
![Western Blot: SETD7 Antibody [NB100-93288] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-93288 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/SETD7-Antibody-Western-Blot-NB100-93288-img0004.jpg "Western Blot: SETD7 Antibody [NB100-93288] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-93288 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.")
![Immunohistochemistry-Paraffin: SETD7 Antibody [NB100-93288] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SET7 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SETD7-Antibody-Immunohistochemistry-Paraffin-NB100-93288-img0005.jpg)
![Flow Cytometry: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C220 (NBP3-20145, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20145_rabbit-setd7-mab-rab-c220-chimeric-azide-and-bsa-free-2742023924289.png "Flow Cytometry: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of RAB-C220 (NBP3-20145, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Nuclear lysate of MCF7(A) (0.01ug/ml), Jurkat(B) (0.1ug/ml) cells and A549(C) (0.03ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C220 (NBP3-20145) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20145_rabbit-setd7-mab-rab-c220-chimeric-azide-and-bsa-free-2742023924219.png)
![Immunocytochemistry/Immunofluorescence: SETD7 Antibody (RAB-C220) - Chimeric - Azide and BSA Free [NBP3-20145] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C220 (NBP3-20145) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20145, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20145_rabbit-setd7-mab-rab-c220-chimeric-azide-and-bsa-free-2742023924216.png)
![Immunocytochemistry/ Immunofluorescence: SETD7 Antibody (PSH02-90) [NBP3-32945] - Immunocytochemistry analysis of HeLa cells labeling SETD7 with Rabbit anti-SETD7 antibody (NBP3-32945) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SETD7 antibody (NBP3-32945) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32945_rabbit-setd7-mab-psh02-90-2920241192368.jpg "Immunocytochemistry/ Immunofluorescence: SETD7 Antibody (PSH02-90) [NBP3-32945] - Immunocytochemistry analysis of HeLa cells labeling SETD7 with Rabbit anti-SETD7 antibody (NBP3-32945) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SETD7 antibody (NBP3-32945) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.")
![Flow Cytometry: SETD7 Antibody (PSH02-90) [NBP3-32945] - Flow cytometric analysis of HeLa cells labeling SETD7. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32945, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32945_rabbit-setd7-mab-psh02-90-29202416372122.jpg)
![Western Blot: SETD7 Antibody (PSH02-90) [NBP3-32945] - Western blot analysis of SETD7 on different lysates with Rabbit anti-SETD7 antibody (NBP3-32945) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-3M cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: PC-12 cell lysate Lane 8: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 50 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32945_rabbit-setd7-mab-psh02-90-3920248453546.jpg)
![Western Blot: SETD7/9 Antibody (s4E5) [NBP1-04298] - The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/SETD7-9-Antibody-s4E5-Western-Blot-NBP1-04298-img0011.jpg "Western Blot: SETD7/9 Antibody (s4E5) [NBP1-04298] - The Recombinant human SET7/9 protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SET7/9 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: SETD7/9 Antibody (s4E5) [NBP1-04298] - Analysis of SET7/9 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SET7/9 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/SETD7-9-Antibody-s4E5-Immunocytochemistry-Immunofluorescence-NBP1-04298-img0008.jpg)
![Flow Cytometry: SETD7/9 Antibody (s4E5) [NBP1-04298] - Analysis of SET7/9 in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/SETD7-9-Antibody-s4E5-Flow-Cytometry-NBP1-04298-img0009.jpg)
![Western Blot: SETD7/9 Antibody (s4E5) [NBP1-04298] - Recombinant human SET7/9 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed at a 1:2000. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. 293T cell lysate Lane 2. SET7/9 transfected 293T cell lysate.](http://images.novusbio.com/fullsize/SETD7-9-Antibody-s4E5-Western-Blot-NBP1-04298-img0007.jpg)
![Western Blot: SETD7/9 Antibody [NBP2-99744] - Anti-SETD7/9 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 53 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/SETD7-9-Antibody-Western-Blot-NBP2-99744-img0004.jpg "Western Blot: SETD7/9 Antibody [NBP2-99744] - Anti-SETD7/9 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 53 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: SETD7/9 Antibody [NBP2-99744] - SETD7/9 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 2 ul anti-SETD7/9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-SETD7/9 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 52 kDa](http://images.novusbio.com/fullsize/SETD7-9-Antibody-Immunoprecipitation-NBP2-99744-img0003.jpg)
![Western Blot: SETD7 Antibody (7Z6I8) [NBP3-16788] - Western blot analysis of extracts of various cell lines, using SETD7 Rabbit mAb (NBP3-16788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/SETD7-Antibody-7Z6I8-Western-Blot-NBP3-16788-img0001.jpg "Western Blot: SETD7 Antibody (7Z6I8) [NBP3-16788] - Western blot analysis of extracts of various cell lines, using SETD7 Rabbit mAb (NBP3-16788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Western Blot: SETD7 Antibody (7Z6I8) [NBP3-16788] - Western blot analysis of lysates from HeLa cells, using SETD7 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16788_rabbit-setd7-mab-7z6i8-8220252010429.jpg)
![Western Blot: SETD7 Antibody [NBP3-03470] - Western blot analysis of extracts of 293T cells, using [KO Validated] SETD7 antibody (NBP3-03470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/SETD7 Antibody-Western Blot-NBP3-03470-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SETD7 Antibody [NBP3-03470] - Western blot analysis of extracts of 293T cells, using [KO Validated] SETD7 antibody (NBP3-03470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: SETD7 Antibody (SR1694) [NBP3-21620] - Western blot analysis using NBP3-21620 in (1) Jurkat cell lysate; (2) HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21620_rabbit-setd7-mab-sr1694-572023152615.jpg "Western Blot: SETD7 Antibody (SR1694) [NBP3-21620] - Western blot analysis using NBP3-21620 in (1) Jurkat cell lysate; (2) HeLa cell lysate.")