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SETD7 Antibody (5B4)

Images

 
Western Blot: SETD7 Antibody (5B4) [H00080854-M01] - SET7 monoclonal antibody (M01), clone 5B4 Analysis of SET7 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: SETD7 Antibody (5B4) [H00080854-M01] - Analysis of monoclonal antibody to SET7 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SETD7 Antibody (5B4) [H00080854-M01] - Analysis of monoclonal antibody to SETD7 on formalin-fixed paraffin-embedded human colon. Antibody concentration 0.5 ug/ml.
Sandwich ELISA: SETD7 Antibody (5B4) [H00080854-M01] - Detection limit for recombinant GST tagged SETD7 is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
5B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SETD7 Antibody (5B4) Summary

Immunogen
SET7 (NP_085151, 257 a.a. ~ 366 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK
Specificity
SET7 - SET domain-containing protein 7
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SETD7
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SETD7 Antibody (5B4)

  • EC 2.1.1.364
  • EC 2.1.1.43
  • H3-K4-HMTase SETD7
  • histone H3-lysine 4-specific methyltransferase
  • histone-lysine N-methyltransferase SETD7
  • KIAA1717
  • KIAA1717SET domain-containing protein 7
  • KMT7
  • KMT7FLJ21193
  • Lysine N-methyltransferase 7
  • SET domain containing (lysine methyltransferase) 7
  • SET7
  • SET7/9
  • SET7/9Histone H3-K4 methyltransferase SETD7
  • SET7Set9
  • SET9
  • SETD7

Background

SET7 is a member of the SET (SuVar3-9, enhancer of Zeste, Trithorax) domain-containing protein family. SET7 functions as a histone methyltransferase that specifically monomethylates lysine-4 of histone H3. SET7 has also been observed to monomethylate non-histone proteins such as p53, TAF10, and the estrogen receptor. The methylating activity of SET7 is essential to cell cycle progression and genomic stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SETD7
Entrez
OMIM
Uniprot