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![Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of Mouse spleen, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Western-Blot-NBP3-15386-img0006.jpg "Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of Mouse spleen, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of NIH-3T3 cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunocytochemistry-Immunofluorescence-NBP3-15386-img0004.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded human liver cancer using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunohistochemistry-Paraffin-NBP3-15386-img0002.jpg)
![Western Blot: MCM3 Antibody (9Z5W4) [NBP3-15386] - Western blot analysis of extracts of various cell lines, using MCM3 Rabbit mAb (NBP3-15386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Western-Blot-NBP3-15386-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunofluorescence analysis of HeLa cells using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunocytochemistry-Immunofluorescence-NBP3-15386-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunohistochemistry of paraffin-embedded rat spleen using MCM3 Rabbit mAb (NBP3-15386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunohistochemistry-Paraffin-NBP3-15386-img0001.jpg)
![Immunoprecipitation: MCM3 Antibody (9Z5W4) [NBP3-15386] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM3 antibody (NBP3-15386). Western blot was performed from the immunoprecipitate using MCM3 antibody (NBP3-15386) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/MCM3-Antibody-9Z5W4-Immunoprecipitation-NBP3-15386-img0007.jpg)
![Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-289] - Detection of Human MCM3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody NB100-289 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-289] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-289-img0010.jpg)
![Western Blot: MCM3 Antibody [NB100-289] - RIPA extract (50 ug) from HeLa cells. Antibody used at 0.2 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-289-img0002.jpg)
![Western Blot: MCM3 Antibody [NBP1-85797] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NBP1-85797-img0005.jpg "Western Blot: MCM3 Antibody [NBP1-85797] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: MCM3 Antibody [NBP1-85797] - Staining of human tonsil shows strong nuclear positivity in reaction center cells.](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-Paraffin-NBP1-85797-img0001.jpg)
![Western Blot: MCM3 Antibody [NBP1-85797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NBP1-85797-img0004.jpg)
![Western Blot: MCM3 Antibody [NBP1-85796] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NBP1-85796-img0007.jpg "Western Blot: MCM3 Antibody [NBP1-85796] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody [NBP1-85796] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & centrosome.](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85796-img0008.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody [NBP1-85796] - Staining of human lymph node shows strong nuclear positivity in reaction center cells.](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-Paraffin-NBP1-85796-img0006.jpg)
![Western Blot: MCM3 Antibody [NBP1-85796] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NBP1-85796-img0005.jpg)
![Western Blot: MCM3 Antibody (S04-6B9) [NBP3-19485] - Western blot detection of MCM3 in Jurkat, C6, Hela cell lysates using NBP3-19485 (1:1000 diluted). Predicted band size: 91kDa. Observed band size: 100kDa.](http://images.novusbio.com/fullsize/MCM3-Antibody-S04-6B9-Western-Blot-NBP3-19485-img0003.jpg "Western Blot: MCM3 Antibody (S04-6B9) [NBP3-19485] - Western blot detection of MCM3 in Jurkat, C6, Hela cell lysates using NBP3-19485 (1:1000 diluted). Predicted band size: 91kDa. Observed band size: 100kDa.")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (S04-6B9) [NBP3-19485] - Immunofluorescence of MCM3 (green) in Hela cells using NBP3-19485 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/MCM3-Antibody-S04-6B9-Immunocytochemistry-Immunofluorescence-NBP3-19485-img0001.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody (S04-6B9) [NBP3-19485] - Immunohistochemistry of MCM3 in paraffin-embedded Human tonsil using NBP3-19485 at dilution 1/1](http://images.novusbio.com/fullsize/MCM3-Antibody-S04-6B9-Immunohistochemistry-Paraffin-NBP3-19485-img0002.jpg)
![Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in human ovarian cancer.](http://images.novusbio.com/fullsize/MCM3-Antibody-2H3-Western-Blot-H00004172-M04-img0009.jpg "Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in human ovarian cancer.")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of monoclonal antibody to MCM3 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MCM3-Antibody-2H3-Immunocytochemistry-Immunofluorescence-H00004172-M04-img0006.jpg)
![Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/MCM3-Antibody-2H3-Western-Blot-H00004172-M04-img0007.jpg)
![Western Blot: MCM3 Antibody (2H3) [H00004172-M04] - Analysis of MCM3 expression in Y-79 (Cat # L042V1).](http://images.novusbio.com/fullsize/MCM3-Antibody-2H3-Western-Blot-H00004172-M04-img0008.jpg)
![ELISA: MCM3 Antibody (2H3) [H00004172-M04] - Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM3-Antibody-2H3-ELISA-H00004172-M04-img0004.jpg)
![Western Blot: MCM3 Antibody [NBP1-33028] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NBP1-33028-img0005.jpg "Western Blot: MCM3 Antibody [NBP1-33028] - Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody [NBP1-33028] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM3 protein stained by MCM3 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33028-img0003.jpg)
![Immunohistochemistry-Paraffin: MCM3 Antibody [NBP1-33028] - Paraffin-embedded NCIN87 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-Paraffin-NBP1-33028-img0004.jpg)
![Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa [L013V1].](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Western-Blot-H00004172-M03-img0010.jpg "Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa [L013V1].")
![Immunocytochemistry/Immunofluorescence: MCM3 Antibody (3E1) [H00004172-M03] - Analysis of monoclonal antibody to MCM3 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Immunocytochemistry-Immunofluorescence-H00004172-M03-img0006.jpg)
![Western Blot: MCM3 Antibody (3E1) [H00004172-M03] - Analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Western-Blot-H00004172-M03-img0009.jpg)
![Sandwich ELISA: MCM3 Antibody (3E1) [H00004172-M03] - Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MCM3-Antibody-3E1-Sandwich-ELISA-H00004172-M03-img0007.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0059.jpg "<b>Biological Strategies Validation. </b>Western Blot: MCM3 Antibody [NB100-249] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: MCM3 Antibody [NB100-249] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified goat anti-MCM3 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM3-Antibody-Immunohistochemistry-NB100-249-img0061.jpg)
![Western Blot: MCM3 Antibody [NB100-249] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.](http://images.novusbio.com/fullsize/MCM3-Antibody-Western-Blot-NB100-249-img0002.jpg)
![SDS-Page: Recombinant Human MCM3 Protein [H00004172-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004172-Q01-1.jpg "SDS-Page: Recombinant Human MCM3 Protein [H00004172-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
