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![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Human Jurkat cell lysate Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0010.jpg "Western Blot: Pannexin-1 Antibody [NBP1-59672] - Human Jurkat cell lysate Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry: Pannexin-1 Antibody [NBP1-59672] - Paraffin Embedded Tissue: Human neural cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-NBP1-59672-img0008.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Tissue: Hela whole cell lysates](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0004.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - PANX1 Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0001.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-59672] - Mouse Retina and Knockout PANX-1 Mouse Tissue.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-59672-img0003.jpg)
![Immunohistochemistry: Pannexin-1 Antibody [NBP1-59672] - Human Intestine](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-NBP1-59672-img0007.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-92241] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PANX1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-92241-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Pannexin-1 Antibody [NBP1-92241] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PANX1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody [NBP1-92241] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92241-img0010.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human small intestine shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0016.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0012.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0014.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human pancreas shows negative to very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0015.jpg)
![Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-278202415212479.jpg "Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32714) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-298202413585529.jpg)
![Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunocytochemistry analysis of 293T cells labeling Pannexin-1 with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-292024111486.jpg)
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-262202414225215.jpg "Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.")
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-2722024847192.jpg)
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody (28G5) [NBP3-26441] - Staining of U251 with Pannexin-1 Antibody (28G5) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 502-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202412493632.jpg)
![Flow Cytometry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Overlay Peak curve showing A549 cells stained with Pannexin-1 Antibody (28G5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202414312766.jpg)
![Western Blot: Pannexin-1 Antibody [NBP2-94536] - Analysis of extracts of various cell lines, using Pannexin-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP2-94536-img0002.jpg "Western Blot: Pannexin-1 Antibody [NBP2-94536] - Analysis of extracts of various cell lines, using Pannexin-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody [NBP2-94536] - Analysis of MCF7 cells using Pannexin-1 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94536-img0001.jpg)
![Western Blot: Pannexin-1 Antibody (7E8T3) [NBP3-16578] - Analysis of extracts of various cell lines, using Pannexin-1 Rabbit mAb (NBP3-16578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-7E8T3-Western-Blot-NBP3-16578-img0002.jpg "Western Blot: Pannexin-1 Antibody (7E8T3) [NBP3-16578] - Analysis of extracts of various cell lines, using Pannexin-1 Rabbit mAb (NBP3-16578) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody (7E8T3) [NBP3-16578] - Human esophageal cancer using Pannexin-1 Rabbit mAb (NBP3-16578) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-7E8T3-Immunohistochemistry-Paraffin-NBP3-16578-img0001.jpg)
![Western Blot: Pannexin-1 Antibody [NBP2-82019] - Analysis of Pannexin-1 in human ovary tissue lysate with Pannexin-1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP2-82019-img0001.jpg "Western Blot: Pannexin-1 Antibody [NBP2-82019] - Analysis of Pannexin-1 in human ovary tissue lysate with Pannexin-1 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody [NBP2-82019] - Immunofluorescence of Pannexin-1 in human ovary tissue with Pannexin-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82019-img0002.jpg)
![Sandwich ELISA: Pannexin-1 Antibody (2E3) [H00024145-M07] - Detection limit for recombinant GST tagged PANX1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-2E3-Sandwich-ELISA-H00024145-M07-img0001.jpg "Sandwich ELISA: Pannexin-1 Antibody (2E3) [H00024145-M07] - Detection limit for recombinant GST tagged PANX1 is 1 ng/ml as a capture antibody.")
![Western Blot: Pannexin-1 Antibody [NBP2-94060] - Analysis of extracts of various cell lines, using Pannexin-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP2-94060-img0001.jpg "Western Blot: Pannexin-1 Antibody [NBP2-94060] - Analysis of extracts of various cell lines, using Pannexin-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.")
![Western Blot: Pannexin 1 Overexpression Lysate (Adult Normal) [NBL1-14093] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pannexin 1.](http://images.novusbio.com/fullsize/Pannexin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14093-img0002.jpg "Western Blot: Pannexin 1 Overexpression Lysate (Adult Normal) [NBL1-14093] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pannexin 1.")