| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1787-MD-050/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77201-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75513-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1787-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1787-SP | |||
![Western Blot: MD-2 Antibody [NB100-56655] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license. anti-TLR4 (Novus Biological NBP2-24538).](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NB100-56655-img0011.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: MD-2 Antibody [NB100-56655] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY license. anti-TLR4 (Novus Biological NBP2-24538).")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: MD-2 Antibody [NB100-56655] - Inactivation of MEK-ERK1/2 and NF-kappaB signalling rescues the adverse effects of MD-1 deficiency on Ang II-induced myocyte hypertrophy. Representative western blot of quantitative analysis of the TLR4 and MD-2 protein levels in WT and MD-1-/- mice at 4 weeks after sham or AB surgery (n = 4). *P < 0.05 vs. WT/shams; #P < 0.05 vs. WT/AB. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep41857) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NB100-56655-img0012.jpg)
![Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655] - Analysis of MD2 in human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at 10 ug/ml.](http://images.novusbio.com/fullsize/MD-2-Antibody-Immunohistochemistry-Paraffin-NB100-56655-img0010.jpg)
![Western Blot: MD-2 Antibody [NB100-56655] - Image of anti-MD2. Total protein from human colon (lane 1) and human spleen (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-MD2 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NB100-56655-img0007.jpg)
![Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Rat Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NBP1-77201-img0012.jpg "Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Rat Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MD-2 recombinant protein per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL and Lane 3: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NBP1-77201-img0007.jpg)
![Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Daudi Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NBP1-77201-img0008.jpg)
![Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NBP1-77201-img0009.jpg)
![Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/MD-2-Antibody-Western-Blot-NBP1-77201-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling MD-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77201_rabbit-polyclonal-md-2-antibody-2711202420392781.gif)
![Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Mouse Spleen Tissue LysateLoading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77201_rabbit-polyclonal-md-2-antibody-2711202419251545.gif)
![Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MD-2 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77201_rabbit-polyclonal-md-2-antibody-271120242074647.gif)
![Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Tissue Lysates.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77201_rabbit-polyclonal-md-2-antibody-271120242022937.gif)
![Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling MD-2 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77201_rabbit-polyclonal-md-2-antibody-2711202420141967.jpg)
![Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen using anti-MD-2 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77201_rabbit-polyclonal-md-2-antibody-2711202420175164.jpg)
![Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Spleen Tissue Lysate.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77201_rabbit-polyclonal-md-2-antibody-2711202419261893.gif)
![Western Blot: MD-2 Antibody (1A2E3) [NBP1-75513] - Analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/MD-2-Antibody-1A2E3-Western-Blot-NBP1-75513-img0003.jpg "Western Blot: MD-2 Antibody (1A2E3) [NBP1-75513] - Analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: MD-2 Antibody (1A2E3) - BSA Free [NBP1-75513] - Immunofluorescence of MD-2 in human spleen with MD-2 Antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp1-75513_mouse-monoclonal-md-2-antibody-1a2e3-2711202419172224.gif)
![Immunocytochemistry/Immunofluorescence: MD-2 Antibody [NBP2-55669] - Staining of human cell line U-2 OS shows localization to vesicles.](http://images.novusbio.com/fullsize/MD-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55669-img0001.jpg "Immunocytochemistry/Immunofluorescence: MD-2 Antibody [NBP2-55669] - Staining of human cell line U-2 OS shows localization to vesicles.")
![Western Blot: MD2 Overexpression Lysate (Adult Normal) [NBL1-12751] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MD2.](http://images.novusbio.com/fullsize/MD2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12751-img0002.jpg "Western Blot: MD2 Overexpression Lysate (Adult Normal) [NBL1-12751] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MD2.")
![ELISA: Human MD-2 ELISA Kit (Colorimetric) [NBP3-40036] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40036_human-md-2-elisa-kit-colorimetric-2110202415403149.png "ELISA: Human MD-2 ELISA Kit (Colorimetric) [NBP3-40036] - Standard Curve Reference")
![ELISA: Human MD-2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40037] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40037_human-md-2-ready-to-use-elisa-kit-colorimetric-2110202415403157.png "ELISA: Human MD-2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40037] - Standard Curve Reference")