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CCL28 Antibody (134306) [Unconjugated]

 (9 Publications)
 (1 Review)
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Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL28 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL28 Monoclonal Antibody (Catalog # MAB533). The ND50 is typically 2.5-5.0 µg/mL.
Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clone
134306
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB533-SP
Carrier Free
MAB533-500
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CCL28 Antibody (134306) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Specificity
Detects mouse CCL28 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) CCL28 is observed and no cross-reactivity with rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10, 11, 12, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CCL28
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Neutralization 2.5-5.0 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB533 in the following applications:
Publications
Read Publications using
MAB533 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL28 Antibody (134306) [Unconjugated]

  • CCL28
  • chemokine (C-C motif) ligand 28
  • MEC
  • member 28
  • MGC71902
  • Protein CCK1
  • VIC

Background

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL28 Antibody (MAB533)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IHC-Fr, Immunoprecipitation, In Vivo, Neutralization.


Filter By Application
IHC-Fr
(1)
Immunoprecipitation
(1)
In Vivo
(2)
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(5)
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Mouse
(9)
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Showing Publications 1 - 9 of 9.
Publications using MAB533 Applications Species
Zhang, S;Li, P;Li, J;Gao, J;Qi, Q;Dong, G;Liu, X;Jiao, Q;Wang, Y;Du, L;Zhan, H;Xu, S;Wang, C; Chromatin accessibility uncovers KRAS-driven FOSL2 promoting pancreatic ductal adenocarcinoma progression through up-regulation of CCL28 British journal of cancer 2023-06-28 [PMID: 37380804] (In Vivo, Mouse) In Vivo Mouse
L Sun, Y Zhang, J Cai, B Rimal, ER Rocha, JP Coleman, C Zhang, RG Nichols, Y Luo, B Kim, Y Chen, KW Krausz, CC Harris, AD Patterson, Z Zhang, S Takahashi, FJ Gonzalez Bile salt hydrolase in non-enterotoxigenic Bacteroides potentiates colorectal cancer Nature Communications, 2023-02-10;14(1):755. 2023-02-10 [PMID: 36765047] (Neutralization, Mouse) Neutralization Mouse
P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019-09-26;11(18):7402-7415. 2019-09-26 [PMID: 31557129] (Neutralization, Mouse) Neutralization Mouse
BJ Buelow, M Rohlfing, F Jung, GJ Douglas, MH Grayson POL7085 or anti-CCL28 treatment inhibits development of post-paramyxoviral airway disease Immun Inflamm Dis, 2017-02-02;5(2):98-108. 2017-02-02 [PMID: 28474501] (In Vivo, Mouse) In Vivo Mouse
Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B Structure-function analysis of CCL28 in the development of post-viral asthma. J Biol Chem, 2015-01-02;290(7):4528-36. 2015-01-02 [PMID: 25556652] (Neutralization, Mouse) Neutralization Mouse
Cha HR, Ko HJ, Kim ED Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. J. Immunol., 2011-08-10;187(6):3044-52. 2011-08-10 [PMID: 21832166] (Neutralization, Mouse) Neutralization Mouse
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011-07-13;475(7355):226-30. 2011-07-13 [PMID: 21753853] (IHC-Fr, Mouse) IHC-Fr Mouse
Mosconi E, Rekima A, Seitz-Polski B Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development. Mucosal Immunol, 2010-05-19;3(5):461-74. 2010-05-19 [PMID: 20485331] (Neutralization, Mouse) Neutralization Mouse
Kutzler MA, Kraynyak KA, Nagle SJ, Parkinson RM, Zharikova D, Chattergoon M, Maguire H, Muthumani K, Ugen K, Weiner DB Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo. Gene Ther., 2009-10-22;17(1):72-82. 2009-10-22 [PMID: 19847203] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse

Review for CCL28 Antibody (MAB533) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB533:
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WB
(1)
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  4
reviewed by:
Verified Customer
 WB Mouse 02/25/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23021255
SpeciesMouse

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Application: WB
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Bioinformatics

Gene Symbol CCL28
Uniprot