| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56576SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-524SS | |||
| Azide and BSA Free | NBP2-80892 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-534SS | |||
| Azide and BSA Free | NBP2-80947 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-167SS | |||
| Azide and BSA Free | NBP2-80826 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-62264-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80918 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52709-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2732SS | |||
| Azide and BSA Free | NBP2-80779 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56743SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-529-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80780 | |||
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0005.jpg "Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Immunostaining of severe acute respiratory syndrome coronavirus 2 in pulmonary tissues from fatal coronavirus disease cases. A) P5 (Patient 5): scattered immunostaining of tracheal epithelial cells. B) P5: higher magnification shows immunostaining of ciliated cells. C) P8: immunostaining of desquamated type I pneumocyte in an alveolar lumen. D) P4: colocalization of SARS-CoV-2 viral antigen (red) with type II pneumocyte stained by surfactant (brown; arrow). E) P4: colocalization of SARS-CoV-2 viral antigen (red) with macrophages stained by CD163 (brown; arrows); virus immunostaining within type II pneumocytes is also seen (arrowheads). F) P4: extensive immunostaining of hyaline membranes in a region of exudative DAD. G) P3: scattered immunostaining within macrophage in hilar lymph node; anthracosis is also present. Emerg Infect Dis. 2020 May 21;26(9) 10.3201/eid2609.202095, PMID: 32437316")
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Pathological changes in rhesus macaques infected with SARS-CoV-2. (g) SARSCoV-2 antigen is detected by immunohistochemistry in type I pneumocytes. Magnification 400x. (j) SARS-CoV-2 antigen is detected by immunohistochemistry in type I pneumocytes (asterisk) and type II pneumocytes (arrow) as well as alveolar macrophages (arrowheads). Magnification 400x. (k) SARS-CoV-2 antigen is detected by immunohistochemistry in mediastinal lymph node. Magnification 400x. (l) SARSCoV-2 antigen is detected by immunohistochemistry in macrophages and lymphocytes in the lamina propria of the cecum. Magnification 400x. bioRxiv March 21, 2020 //doi.org/10.1101/2020.03.21.001628](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: SARS Nucleocapsid Protein Antibody [NB100-56576] - Formalin-fixed paraffin-embedded tissue sections of SARS-CoV-2 infected human lung tissue were probed for SARS-CoV-2 viral RNA (ACD anti-sense specific probe v-nCoV2019-S (848561); Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal anti-SARS Nucleocapsid Antibody [NB100-56576] at 15ug/mL with 1 hr incubation at 25 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Dual-RNAscope-ISH-IHC-NB100-56576-img0006.jpg)
![Immunohistochemistry: SARS Nucleocapsid Protein Antibody [NB100-56576] - Characteristics of 2B4 cells clonally derived from human bronchial epithelial Calu-3 cells. Finally, 2B4 cells (passage 6) were infected with SARS-CoV (MOI = 0.1) for 24, 48, and 72 hrs before being fixed with 4% paraformaldehyde for monitoring the morphological changes of infected cells, as visualized by the expression of SARS-CoV NP protein (red) by using the standard IHC (F). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0008729) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Immunohistochemistry-NB100-56576-img0003.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-SARS-CoV-2 Nucleocapsid Polyclonal Antibody (Catalog # NB100-56576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for SARS-CoV-2 Nucleocapsid at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0007.jpg)
![Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56576] - analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody.](http://images.novusbio.com/fullsize/SARS-Nucleocapsid-Protein-Antibody-Western-Blot-NB100-56576-img0002.jpg)
![Immunohistochemistry-Paraffin: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis of a FFPE tissue section of the mouse colon using 1:200 dilution of O-GlcNAc [RL2] antibody (NB300-524). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Immunohistochemistry-Paraffin-NB300-524-img0011.jpg "Immunohistochemistry-Paraffin: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis of a FFPE tissue section of the mouse colon using 1:200 dilution of O-GlcNAc [RL2] antibody (NB300-524). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.")
![Western Blot: O-GlcNAc Antibody (RL2) [NB300-524] - Impact of OGA inhibition by thiamet G in primary cultures of NCM. Representative western blots (left panel) and quantification (right panel) of O-GlcNAcylated proteins levels in control (Ctrl) and NCM treated with 100 nM of thiamet G (TG) during 24 h (n = 12). Image collected and cropped by Citeab from the following publication (Interplay Between Phosphorylation and O-GlcNAcylation of Sarcomeric Proteins in Ischemic Heart Failure. Front Endocrinol (Lausanne) (2018) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Western-Blot-NB300-524-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (RL2) [NB300-524] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-O-GlcNAc (RL2) at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Immunocytochemistry-Immunofluorescence-NB300-524-img0013.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Neuro2a cells with O-GlcNAc Antibody [RL2] NB300-524 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0018.jpg)
![Western Blot: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis of mouse cortical brain lysates using O-Linked N-Acetylglucosamine Monoclonal Antibody. Blots containing cortical extracts from 4 individual C57BL/6 mice (Lanes 1-4) were blocked with 5% milk in TBST, and probed with MA1-072 at 1:1000, followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Western-Blot-NB300-524-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: O-GlcNAc Antibody (RL2) [NB300-524] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-O-GlcNAc (RL2) at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Immunocytochemistry-Immunofluorescence-NB300-524-img0012.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - Analysis using Alexa Fluor (R) 647 conjugate of NB300-524. An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0006.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on U-937 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0007.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Jurkat cells with O-GlcNAc antibody (RL2) NB300-524PE (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0008.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on SK-MEL-28 cells with O-GlcNAc antibody (RL2) NB300-524AF647 (blue) and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature . Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0009.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on HeLa cells with O-GlcNAc Antibody [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0010.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Neuro2a cells with O-GlcNAc Antibody [RL2] NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0014.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on RH30 cells with O-GlcNAc [RL2] Antibody NB300-524AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0015.jpg)
![Flow Cytometry: O-GlcNAc Antibody (RL2) [NB300-524] - An intracellular stain was performed on Jurkat cells with O-GlcNAc Antibody [RL2] NB300-524 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/O-GlcNAc-Antibody-RL2-Flow-Cytometry-NB300-524-img0017.jpg)
![Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Immunohistochemistry-Frozen-NB600-534-img0003.jpg "Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.")
![Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).](http://images.novusbio.com/fullsize/Siglec-1-CD169-Antibody-HSn-7D2-Flow-Cytometry-NB600-534-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Sialoadhesin-Antibody-HSn-7D2-Immunohistochemistry-Paraffin-NB600-534-img0001.jpg)
![Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Western-Blot-NB200-167-img0003.jpg "Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)")
![Immunohistochemistry-Paraffin: LANA2 Antibody (CM-A807) [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource and performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA. Representative image shows the LANA staining of skin KS lesion. Image collected and cropped by CiteAb from the following publication (//mbio.asm.org/lookup/doi/10.1128/mBio.02217-17), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LANA2-Antibody-CM-A807-Immunohistochemistry-Paraffin-NB200-167-img0004.jpg)
![Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - Analysis using the Biotin conjugate of NB100-62264. Detection of V5 tagged protein with Mouse anti V5-tag.](http://images.novusbio.com/fullsize/Paramyxovirus-SV5-Pk-Antibody-SV5-Pk1-Western-Blot-NB100-62264-img0004.jpg "Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - Analysis using the Biotin conjugate of NB100-62264. Detection of V5 tagged protein with Mouse anti V5-tag.")
![Sandwich ELISA: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - V5 tagged protein detected with Mouse anti V5-Tag.](http://images.novusbio.com/fullsize/Paramyxovirus-SV5-Pk-Antibody-SV5-Pk1-Sandwich-ELISA-NB100-62264-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum. Image from verified customer review.](http://images.novusbio.com/fullsize/Polyoma-Virus-Medium-T-Antigen-Antibody-PyMT-Immunocytochemistry-Immunofluorescence-NB100-2749-img0001.jpg "Immunocytochemistry/Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. Mammary tumor samples were formalin-fixed and serial sections were stained for Darpp-32 and PyMT (5x magnification in a 6 x 6 tile, scale bar = 2000 um; images enlarged from the boxed regions are at 10x magnification, scale bar = 200 um). Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-192), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Polyoma-Virus-Medium-T-Antigen-Antibody-PyMT-Immunohistochemistry-Paraffin-NB100-2749-img0002.jpg)
![Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - AREG not expressed in PyMT lesions. Representative images of PyMT immunofluorescent staining of 6-week-old AREG +/+ PyMT and AREG -/- PyMT MFPs, respectively. Scale bar shows 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30367629/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Polyoma-Virus-Medium-T-Antigen-Antibody-PyMT-Immunohistochemistry-NB100-2749-img0003.jpg)
![Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [PE] [NBP2-52709PE] - Mk2 cell line was infected with ZIKA virus at 72 p.o.i and the sample was stained and analyzed by flow cytometry. Image from verified customer review. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Flow-Cytometry-NBP2-52709-img0003.jpg)
![Western Blot: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus West Nile Virus MP in (A) untransfected and (B) transfected mouse melanoma cells using NB100-56743 at 1 ug/ml.](http://images.novusbio.com/fullsize/West-Nile-Virus-MP-Antibody---BSA-Free-Western-Blot-NB100-56743-img0007.jpg "Western Blot: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus West Nile Virus MP in (A) untransfected and (B) transfected mouse melanoma cells using NB100-56743 at 1 ug/ml.")
![Immunohistochemistry-Paraffin: West Nile Virus MP Antibody - BSA Free [NB100-56743] - Analysis of West Nile Virus MP in bird liver using this antibody at 1 ug/ml, incubated for 30 minutes. A Ventana brand second step antibody conjugated to biotin was applied followed with streptavidin-AKP. The substrate was Fast Red chromagen. The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip. (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.](http://images.novusbio.com/fullsize/West Nile Virus MP Antibody - BSA Free-Immunohistochemistry-Paraffin-NB100-56743-img0008.jpg)
![Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands are also detected.](http://images.novusbio.com/fullsize/p35-baculovirus-Antibody-Western-Blot-NB100-56153-img0002.jpg "Western Blot: p35 baculovirus Antibody [NB100-56153] - Analysis of in vitro translated p35 baculovirus protein using this antibody. Full-length p35 is detected at ~35 kDa, lower molecular p35 breakdown/cleavage bands are also detected.")
![Western Blot: SERCA2 ATPase Antibody (IID8) [NB300-529] - Analysis of 25 ug of rat skeletal muscle (lane 1) and human skeletal muscle (lane 2).](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-IID8-Western-Blot-NB300-529-img0010.jpg "Western Blot: SERCA2 ATPase Antibody (IID8) [NB300-529] - Analysis of 25 ug of rat skeletal muscle (lane 1) and human skeletal muscle (lane 2).")
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [NB300-529] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-IID8-Immunocytochemistry-Immunofluorescence-NB300-529-img0006.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [NB300-529] - Both normal and cancer biopsies of deparaffinized human Heart tissue.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-IID8-Immunohistochemistry-Paraffin-NB300-529-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [NB300-529] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-IID8-Immunocytochemistry-Immunofluorescence-NB300-529-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [NB300-529] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-IID8-Immunocytochemistry-Immunofluorescence-NB300-529-img0005.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [NB300-529] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-IID8-Immunohistochemistry-Paraffin-NB300-529-img0007.jpg)
![Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [NB300-529] - Both normal and cancer biopsies of deparaffinized human Liver tissue.](http://images.novusbio.com/fullsize/SERCA2-ATPase-Antibody-IID8-Immunohistochemistry-Paraffin-NB300-529-img0008.jpg)