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Paramyxovirus SV5 Pk Antibody (SV5-Pk1)

Images

 
Western Blot: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - Analysis using the Biotin conjugate of NB100-62264. Detection of V5 tagged protein with Mouse anti V5-tag.
Sandwich ELISA: Paramyxovirus SV5 Pk Antibody (SV5-Pk1) [NB100-62264] - V5 tagged protein detected with Mouse anti V5-Tag.

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC, IP
Clone
SV5-Pk1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Paramyxovirus SV5 Pk Antibody (SV5-Pk1) Summary

Immunogen
Paramyxovirus Simian-Virus 5 (SV5)
Specificity
This product recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NB100-62264 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro (ile pro asn pro leu leu gly leu asp) ser thr. (9 amino acid epitope is in parenthesis)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000-1:5000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000
  • Radioimmunoassay 1:100-1:2000
  • Sandwich ELISA
  • Western Blot 1:1000-1:5000
Publications
Read Publications using
NB100-62264 in the following applications:

Reactivity Notes

Epitope tag. Non-species specific. Mouse reactivity reported in scientific literature (PMID: 27681617).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Paramyxovirus SV5 Pk Antibody (SV5-Pk1)

  • Simian virus 5; SPV5gp2; V protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Paramyxovirus SV5 Pk Antibody (NB100-62264)(24)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(3)
IF/IHC
(1)
IHC-Fr
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-62264 Applications Species
Rao D, Ferguson R, Sikora M. WNT4 and WNT3A activate cell autonomous Wnt signaling independent of secretion J Biol Chem 2019-11-20 [PMID: 31740580]
Yang C, Danielson EW, Qiao T et al. Mutant PFN1 causes ALS phenotypes and progressive motor neuron degeneration in mice by a gain of toxicity. Proc. Natl. Acad. Sci. U.S.A. 2016-10-11 [PMID: 27681617] (ICC/IF, IF/IHC, Mouse) ICC/IF, IF/IHC Mouse
Walter D, Hoffmann S, Komseli ES et al. SCF(Cyclin F)-dependent degradation of CDC6 suppresses DNA re-replication. Nat Commun. 2016-01-28 [PMID: 26818844] (ICC/IF, WB) ICC/IF, WB
Ghosh S, Wu MD, Shaftel SS et al. Sustained interleukin-1beta overexpression exacerbates tau pathology despite reduced amyloid burden in an Alzheimer's mouse model. J Neurosci 2013-03-13 [PMID: 23486975] (ICC/IF, IHC-Fr) ICC/IF, IHC-Fr
Gatherer, D et al. High-resolution human cytomegalovirus transcriptome. Proc Natl Acad Sci U S A. 2011-11-22 [PMID: 22109557]
Patino, GA et al. Voltage-Gated Na+ Channel {beta}1B: A Secreted Cell Adhesion Molecule Involved in Human Epilepsy. J Neurosci 31: 14577-91. 2011-01-01 [PMID: 21994374]
Liebau, MC et al. Nephrocystin-4 regulates Pyk2-induced tyrosine phosphorylation of Nephrocystin-1 to control targeting to monocilia. J Biol Chem. 2011-02-28 [PMID: 21357692]
Gallazzini, M et al. High NaCl-induced activation of CDK5 increases phosphorylation of the osmoprotective transcription factor TonEBP/OREBP at threonine 135, which contributes to its rapid nuclear localization. Mol Biol Cell 22: 703-14. 2011-01-01 [PMID: 21209322]
Herskowitz, JH et al. Rho Kinase II Phosphorylation of the Lipoprotein Receptor LR11/SORLA Alters Amyloid-{beta} Production. J Biol Chem 286: 6117-27. 2011-01-01 [PMID: 21147781]
Zhao, A et al. Rapid isolation of high-affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast-display/secretory scFv library platform. J Immunol Methods 363: 221-32. 2011-01-01 [PMID: 20837020]
Show All 24 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Paramyxovirus SV5 Pk Antibody (NB100-62264). (Showing 1 - 1 of 1 FAQs).

  1. I can't find any images or the antibody used in publications for this application, but the description says it works in this particular application. Could the company provide additional data?
    • This antibody has been validated in IHC on both frozen and paraffin-embedded tissues and is 100% guaranteed for this use. Validation came from customer feedback, so unfortunately, these images are not available. I apologize for the inconvenience. If you are concerned about images, you may want to consider NB600-381 or NB100-62264 as these two products both have over 20 publications.

Secondary Antibodies

 

Isotype Controls

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