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145 Results for "novus"

NB300-221

GAPDH Antibody (1D4)

(19 Reviews)

(411 Publications)

0.1 ml

NB300-221

Analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.

Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDH antibody produces strong cytoplasmic staining of healthy cells.

Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.

Human eye cell lysate. Antibody used at 1:500. WB image submitted by a verified customer review.

Analysis of GAPDH in chicken pectoralis major using anti-GAPDH antibody. The primary antibody was used at a dilution of 1:4000, incubated for 1 hour at room temperature. Western blot image submitted by a verified customer review.

Application of the approach to other cell lines. The remainder of the protocol from Figure 2 was then applied using 100 ug/mL digitonin as the optimal cytosolic extract in each case. An aliquot of each extract was then analyzed by 4-12% SDS PAGE followed by Western blotting and probing with antibodies to GAPDH, BiP and Histone H3 (Used as an alternative nuclear marker due to weakness of the Lamin A signal in HeLa cells). Image collected and cropped by CiteAb from the following publication (//bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-2-243) licensed under a CC-BY license.

Endothelial responses provoked by Hb-lipid interactions are attenuated by sulfide donors. Confluence HAoECs were exposed to the obtained Hb samples for 8 hours and VCAM-1 expression was determined by Western blotting. GAPDH were used as a loading control. Representative experiment, n = 3. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2018/3812568/), licensed under a CC-BY license.

Regulation of cell migration-associated proteins by NCKU-21 in A549 and CL1-5 cells. Protein expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 were analyzed in A549 cell treated with NCKU-21 for 24 hr. * P 80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected and cropped by CiteAb from the following open publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunodepletions and the effects of RNases and various transcription inhibitors on chromosome condensation and cell cycle state.(A) Western blot for CAP-E, XPB, and Tubulin in Mock or CAP-E depleted extracts. Dilution series of Mock-depleted extracts was used to calculate the % depletion of CAP-E using quantitative fluorescence. (B) 1% agarose gel stained with SYBR safe stain showing RNase A dependent removal of total RNAs from high-speed supernatant egg extracts. (C) Representative fluorescence images of chromatid assembly at steady state (180 min after sperm addition) in the presence of indicated buffers and enzymes. See Materials and methods for details. Note that the high salt in the RNase H buffer causes morphological changes to chromatids regardless of activity. Mean condensation parameters for each condition are indicated in lower left corner of each image. (D) Western blot for XPB and Tubulin in IgG or XPB-depleted extracts. Dilution series of IgG-depleted extracts was used to calculate the % depletion of XPB using quantitative fluorescence. (E) Western blot for XPF, ERCC1, and Tubulin in IgG or ERCC1-depleted extracts. Dilution series of IgG-depleted extracts was used to calculate the % depletion of ERCC1 and XPF using quantitative fluorescence. (F) Representative fluorescence images of chromatid assembly at steady state (180 min after sperm addition) in the presence of DMSO, 30 μM CDK7 inhibitor THZ1, or 30 μM THZ1 and 50 μM triptolide (TPL). (G) Triptolide does not affect CDK1 activation or maintenance of M phase. Western blot for CDK1 T161ph, histone H3T3ph, and Tubulin in M phase HSS extracts in the presence of DMSO or 50 μM triptolide. Both CDK1T161ph (a CDK7 substrate) and H3T3ph are only highly phosphorylated in M phase, and their appearance is not affected by triptolide treatment.Figure 1—figure supplement 1—source data 1.Source data for Figure 1—figure supplement 1A, B,D, E, G.Source data for Figure 1—figure supplement 1A, B,D, E, G. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35293859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Activation of the ATM‐Chk2‐p53 pathway upon CDDP treatmentAConfocal images showing the basal region of organ of Corti cultures treated with either culture medium alone or medium containing 10 μM CDDP for 1 day and immunolabeled for myosin 7A (red) and p‐ATM (green). Scale bar = 15 μm.BHigher magnification images showing representative OHC and IHC nuclei from control and CDDP‐exposed cells. Scale bar = 5 μm.CHistograms displaying green fluorescent signal intensity of x‐projections of OHC and IHC nuclei from control and CDDP‐exposed cells.DQuantification analysis of p‐ATM foci numbers in both OHCs and IHCs exposed to either culture medium alone (light blue and red lines for OHCs and IHCs, respectively) or 10 μM CDDP (blue and red lines for OHCs and IHCs, respectively; n = 50 nuclei per condition and per time point). Data are expressed as mean ± SEM. One‐way ANOVA test followed by post hoc Tukey's test (**P < 0.008, ***P = 0.00005; CDDP‐exposed OHCs versus CDDP‐exposed IHCs).EHigher magnification confocal and 3D images showing the co‐localization of p‐ATM foci (green) with 53BP1 foci (red) in an OHC nucleus from the basal region of the organ of Corti culture treated with 10 μM CDDP for 1 day. Scale bar = 5 μm.FConfocal images showing the basal region of organ of Corti cultures treated with either culture medium alone or medium containing 10 μM CDDP for 1 day and immunolabeled for myosin 7A (red) and p‐Chk2 (green). Scale bar = 15 μm.GHistograms of green fluorescent signal intensity from OHC and IHC nuclei of a CDDP‐treated organ of Corti explant.HQuantification of Chk2‐positive nuclei in both IHCs (red bars) and OHCs (blue bars) from control and CDDP‐treated organ of Corti cultures (n = 50 nuclei per condition and per time point). Data are expressed as mean ± SEM. Kruskal–Wallis test followed by post hoc Dunn's test (*P = 0.045, ***P = 0.0002; CDDP‐exposed IHCs versus control IHCs, CDDP‐exposed OHCs versus control OHCs).IRepresentative Western blots using antibodies against p‐p53 (serine 15), p53, and beta ‐actin in control and 10 μM CDDP‐exposed whole cochlear extracts.J, KHistograms representing the levels of total p53 protein (J) and p53 phosphorylation (K) in control (gray bars) and CDDP‐exposed cochleae (green bars; n = 6 cochleae per condition and per time point). Actin served as a loading control. Data are expressed as mean ± SEM. One‐way ANOVA test followed by post hoc Tukey's test (J: *P ≤ 0.023; K: *P = 0.032, **P = 0.007, ***P ≤ 0.0008; cochleae at the different times after CDDP exposure versus control 12 h).Data information: All experiments were performed in triplicate. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27794029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB110-39115

COX4 Antibody - BSA Free

(4 Reviews)

(17 Publications)

0.1 ml

NB110-39115

$Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Increased mitochondrial localization of CREB under hypoxiaA. Mitochondria were sub fractionated into outer membrane (OM), inter membrane space (IMS), inner membrane (IM) and mitochondrial matrix (MM). The purity of the fractions was analysed with marker proteins: TOM20 for OM, AIF for IMS, COX4 for IM, PDK4 for MM. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/10474/text/) licensed under a CC-BY license.$

Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-COX4 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in human breast cancer using DAB with hematoxylin counterstain.

<b>Biological Strategies Validation. </b>Western Blot: COX4 Antibody - BSA Free [NB110-39115] - The mRNA and protein expression of cytochrome c oxidase 4 (COX4), toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), and nuclear factor (NF)-kB in lung tissues from spontaneous breathing group (CON group), normal tidal volume (NTV) group, high tidal volume (HTV) group, chloroquine (CQ) pretreatment upon HTV stimulation group (HTV + CQ). Levels of COX4, TLR9, MyD88, and NF-kB protein by Western blot. Fold expression for target genes was normalized to that measured for the beta-actin gene. Both of these experiments were in triplicate. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30018615/) licensed under a CC-BY license.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in the following cell lysates: 1. HeLa, 2. Ntera, 3. A431, 4. HepG2, 5. MCF7 and 6. 3T3.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in porcine whole skeletal muscle lysate using anti-COX4 antibody. Image from verified customer review.

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Detection of COX4-1 isoform using COX4 antibody NB110-39115. Lane 1: HEK 293 with empty vector, Lane 2: HEK 293 with flag-tagged human COX4-1, Lane 3: HEK 293 with flag-tagged human COX4-2. Photo courtesy of Ryo Fukuda, G. Semenza lab. Johns Hopkins, SOM

Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Total protein from HeLa, 3T3 and PC12 was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-COX4 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - COX4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - Analysis of HeLa cells using COX4 antibody (NB110-39115, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

Immunocytochemistry: COX4 Antibody - BSA Free [NB110-39115] - Analysis of methanol fixed drosophila embryo using 1:200 dilution of COX4 antibody. The signal was developed using AF488 conjugated Donkey anti-Rabbit IgG (H+L) secondary antibody and the sections were further counterstained for tubulin and DAPI. The antibody generated a specific staining of COX4 in mitochondria near mitotic spindles at early stage of embryo development. Image from verified customer review.

Simple Western: COX4 Antibody - BSA Free [NB110-39115] - Image shows a specific band for COX4 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Small interference RNA targeting Raptor and Rictor disrupts BMAL1 accumulation in HNSCCTargeted disruption of Raptor (A-B) and Rictor (C-D) using siRNA results in a dose-dependent downregulation of BMAL1 in HNSCC cells. E. Disruption of PTEN by protein oxidation causes activation of mTOR signaling, resulting in accumulation of BMAL1. Notably, inhibition of mTOR signaling, particularly mTORC1 and mTORC2, results in restoration of normal BMAL1 levels in the epidermis of mice and head and neck cancer cells. These results demonstrate a novel role for mTOR in regulating nuclear levels of the core clock gene BMAL1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Dr, I, Ma-Op, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Dr, I, Ma-Op, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-39115AF350
Alexa Fluor 405	NB110-39115AF405
Alexa Fluor 488	NB110-39115AF488
Alexa Fluor 532	NB110-39115AF532
Alexa Fluor 594	NB110-39115AF594
Alexa Fluor 647	NB110-39115AF647
Alexa Fluor 700	NB110-39115AF700
Alexa Fluor 750	NB110-39115AF750
Allophycocyanin	NB110-39115APC
Biotin	NB110-39115B
DyLight 350	NB110-39115UV
DyLight 405	NB110-39115V
DyLight 488	NB110-39115G
DyLight 550	NB110-39115R
DyLight 594	NB110-39115DL594
DyLight 650	NB110-39115C
DyLight 680	NB110-39115FR
DyLight 755	NB110-39115IR
FITC	NB110-39115F
HRP	NB110-39115H
Janelia Fluor 549	NB110-39115JF549
Janelia Fluor 646	NB110-39115JF646
PE	NB110-39115PE
PerCP	NB110-39115PCP
mFluor Violet 450 SE	NB110-39115MFV450
mFluor Violet 500 SE	NB110-39115MFV500
mFluor Violet 610 SE	NB110-39115MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-39115SS

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NB110-81529

NHE3/SLC9A3 [p Ser552] Antibody (14D5)

(1 Review)

(16 Publications)

0.1 ml

NB110-81529

Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.

Immunohistochemistry: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.

Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt, Po, Ma-Op, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ma-Op, Pm, Rb

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-81529AF350
Alexa Fluor 405	NB110-81529AF405
Alexa Fluor 488	NB110-81529AF488
Alexa Fluor 532	NB110-81529AF532
Alexa Fluor 594	NB110-81529AF594
Alexa Fluor 647	NB110-81529AF647
Alexa Fluor 700	NB110-81529AF700
Alexa Fluor 750	NB110-81529AF750
Biotin	NB110-81529B
DyLight 350	NB110-81529UV
DyLight 405	NB110-81529V
DyLight 488	NB110-81529G
DyLight 550	NB110-81529R
DyLight 594	NB110-81529DL594
DyLight 650	NB110-81529C
DyLight 680	NB110-81529FR
DyLight 755	NB110-81529IR
FITC	NB110-81529F
HRP	NB110-81529H
Janelia Fluor 549	NB110-81529JF549
Janelia Fluor 646	NB110-81529JF646
mFluor Violet 450 SE	NB110-81529MFV450
mFluor Violet 500 SE	NB110-81529MFV500
mFluor Violet 610 SE	NB110-81529MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80877

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NBP2-24659

SOX9 Antibody

(1 Review)

(4 Publications)

0.1 mg

NBP2-24659

Simple Western: SOX9 Antibody [NBP2-24659] - Simple Western lane view shows a specific band for SOX9 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry: SOX9 Antibody [NBP2-24659] - Osteo-chondroprogenitor cells in the zone adjacent to the titanium plate surface are positive for Sox9 (arrows). Picture on the left represent Group 0.1, pictures on the right represent Group 0.6. 100-fold (c-d) magnification. PLoS One. 2018 Apr 6;13(4):e0195466. doi: 10.1371/journal.pone.0195466.

<b>Biological Strategies Validation. </b>Immunohistochemistry: SOX9 Antibody [NBP2-24659] - 3'-Sialyllactose regulates anabolic and catabolic factor expression via Erk and NF-kB pathways. (C) Immunohistochemical staining of Sox9 in DMM-induced OA mice with administration of PBS and 3'-sialyllactose. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/jcmm.13292), licensed under a CC-BY license.

Immunohistochemistry-Frozen: SOX9 Antibody [NBP2-24659] - Feline optic nerve tissue section was incubated with antibody at 1:200 and further stained with Alexa secondary antibody. Image was captured with an epifluorescence microscope. This image was submitted via customer review.

Western Blot: SOX9 Antibody [NBP2-24659] - Analysis of SOX9 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using SOX9 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Western Blot: SOX9 Antibody [NBP2-24659] - Analysis of SOX9 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using SOX9 antibody at 3 ug/ml.

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP2-24659] - Analysis of SOX9 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top left) and SOX9 antibody (bottom left, right) at 5 ug/ml.

3'-Sialyllactose regulates anabolic and catabolic factor expression via Erk and NF-kappaB pathways. (C) Immunohistochemical staining of Sox9, IkappaB and pErk in DMM-induced OA mice with administration of PBS and 3'-sialyllactose. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28782172), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, Ca, Fe, Gt, Pm, Sh, Bv, Pm, Eq, Pm, Ma-Op

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Fe, Gt, Pm, Sh, Bv, Pm, Eq, Pm, Ma-Op

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6489A

Alt Catalog# IMG-6489A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24659AF350
Alexa Fluor 405	NBP2-24659AF405
Alexa Fluor 488	NBP2-24659AF488
Alexa Fluor 532	NBP2-24659AF532
Alexa Fluor 594	NBP2-24659AF594
Alexa Fluor 647	NBP2-24659AF647
Alexa Fluor 700	NBP2-24659AF700
Alexa Fluor 750	NBP2-24659AF750
Allophycocyanin	NBP2-24659APC
Biotin	NBP2-24659B
DyLight 350	NBP2-24659UV
DyLight 405	NBP2-24659V
DyLight 488	NBP2-24659G
DyLight 550	NBP2-24659R
DyLight 594	NBP2-24659DL594
DyLight 650	NBP2-24659C
DyLight 680	NBP2-24659FR
DyLight 755	NBP2-24659IR
FITC	NBP2-24659F
HRP	NBP2-24659H
Janelia Fluor 549	NBP2-24659JF549
Janelia Fluor 646	NBP2-24659JF646
PE	NBP2-24659PE
PerCP	NBP2-24659PCP
mFluor Violet 450 SE	NBP2-24659MFV450
mFluor Violet 500 SE	NBP2-24659MFV500
mFluor Violet 610 SE	NBP2-24659MFV610

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NBP2-24583

CDX2 Antibody

0.1 mg

NBP2-24583

Western Blot: CDX2 Antibody [NBP2-24583] - Total protein from human HeLa, HepG2, CaCo2 and HCT116, mouse 3T3 and Neuro2A and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CDX2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.

Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583] - Formalin-fixed, paraffin-embedded human spleen probed with CDX2 / CDX3 antibody at 5 ug/ml.

Western Blot: CDX2 Antibody [NBP2-24583] - Analysis of CDX3/CDX2 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Reactivity:

Hu, Mu, Rt, Bv, Eq, Ma-Op, Pm, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Eq, Ma-Op, Pm, Pm, RM

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6520A

Alt Catalog# IMG-6520A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-24463

STAT3 [p Tyr705] Antibody

(3 Reviews)

(4 Publications)

0.02 mg

NBP2-24463

Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of phospho-Stat3 was detected. Image from verified customer review.

Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Analysis of STAT3 (Tyr705) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 705 and 2) an unphosphorylated tyrosine at position 705, using STAT3 phospho antibody at 0.1 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Western Blot: STAT3 [p Tyr705] Antibody [NBP2-24463] - Human breast cancer cells MDA-MB-231 were treated with carboplatin for 72 hours and the expression of p-Stat3 at Y705 and total Stat3 were detected. Image from verified customer review.

Reactivity:

Hu, Mu, Rt, Ca, Ch, Pm, Ma-Op

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ch, Pm, Ma-Op

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6382A

Alt Catalog# IMG-6382A

Conjugate:

Unconjugated

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NLS1380

CXCR4 Antibody - BSA Free

(1 Review)

(5 Publications)

0.05 ml

NLS1380

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human tonsil.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma

Reactivity:

Hu, Mu, Rt, Ch, Ha, Pm, Pm, Rb, Pm, Ma-Op, Pm-Or, Tr

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Ha, Pm, Pm, Rb, Pm, Ma-Op, Pm-Or, Tr

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-24529

eIF4A2 Antibody

(1 Review)

(1 Publication)

0.1 mg

NBP2-24529

Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.

Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Ca, Ch, Pm, Rt, Bv, Eq, Pm, Ma-Op, RM

Species Glossary

Reactivity: Hu, Mu, Ca, Ch, Pm, Rt, Bv, Eq, Pm, Ma-Op, RM

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6278A

Alt Catalog# IMG-6278A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-24531

hnRNP K Antibody

(1 Review)

(1 Publication)

0.1 mg

NBP2-24531

Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis in HeLa nuclear Extracts.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24531] - Formalin-fixed, paraffin-embedded human adrenal tissue probed with hnRNPK antibody at 5 ug/ml.

Western Blot: hnRNP K Antibody [NBP2-24531] - Analysis of hnRNPK in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this experiment.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24531] - Formalin-fixed, paraffin-embedded human thymus probed with hnRNPK antibody at 5 ug/ml.

Reactivity:

Hu, Mu, Rt, Ma-Op, Pm, Bv, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ma-Op, Pm, Bv, Ca, Pm, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6562A

Alt Catalog# IMG-6562A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-23673

ZBTB7A/Pokemon Antibody

(1 Review)

(1 Publication)

0.1 ml

NBP2-23673

Western Blot: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of Pokemon in MCF7 cell lysate. Lane 1 shows pre-immune sera and lane 2 shows Pokemon antibody tested on MCF7 at 1:1000 dilution.

Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of human skin using Pokemon antibody at 1:100.

Reactivity:

Hu, Mu, Rt, Ch, Ma-Op, Pm, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Ma-Op, Pm, Bv, Eq

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6039A

Alt Catalog# IMG-6039A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-27202

14-3-3 Antibody

(1 Review)

0.1 mg

NBP2-27202

Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6796A

Alt Catalog# IMG-6796A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-24486

SOX1 Antibody

(1 Review)

(1 Publication)

0.1 mg

NBP2-24486

Western Blot: SOX1 Antibody [NBP2-24486] - Analysis of extracts from mouse brain using SOX1 antibody (NBP2-24486, 1:100). Image from verified customer review.

Western Blot: SOX1 Antibody [NBP2-24486] - Analysis of SOX1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Western Blot: SOX1 Antibody [NBP2-24486] - Analysis in D3 cell lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24486 at 1 ug/mL. Goat anti-rabbit Ig HRP secondary antibody (Novus, 20301) and PicoTect ECL substrate solution (Novus, 10087K) were used.

Reactivity:

Hu, Mu, Eq, Ma-Op, Pm, Xp, Rt, Bv, Ca, Fe

Species Glossary

Reactivity: Hu, Mu, Eq, Ma-Op, Pm, Xp, Rt, Bv, Ca, Fe

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6446A

Alt Catalog# IMG-6446A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24486AF350
Alexa Fluor 405	NBP2-24486AF405
Alexa Fluor 488	NBP2-24486AF488
Alexa Fluor 532	NBP2-24486AF532
Alexa Fluor 594	NBP2-24486AF594
Alexa Fluor 647	NBP2-24486AF647
Alexa Fluor 700	NBP2-24486AF700
Alexa Fluor 750	NBP2-24486AF750
Allophycocyanin	NBP2-24486APC
Biotin	NBP2-24486B
DyLight 350	NBP2-24486UV
DyLight 405	NBP2-24486V
DyLight 488	NBP2-24486G
DyLight 550	NBP2-24486R
DyLight 594	NBP2-24486DL594
DyLight 650	NBP2-24486C
DyLight 680	NBP2-24486FR
DyLight 755	NBP2-24486IR
HRP	NBP2-24486H
Janelia Fluor 549	NBP2-24486JF549
Janelia Fluor 646	NBP2-24486JF646
PE	NBP2-24486PE
PerCP	NBP2-24486PCP
mFluor Violet 450 SE	NBP2-24486MFV450
mFluor Violet 500 SE	NBP2-24486MFV500
mFluor Violet 610 SE	NBP2-24486MFV610

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