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NB100-143

Nbs1 Antibody

(8 Reviews)

(199 Publications)

0.05 ml

NB100-143

Knockout Validated: Nbs1 Antibody [NB100-143] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Polyclonal [NB100-143] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody [NB100-143] - Western Blot with Nbs1 Antibody [NB100-143]. KSHV LANA recruits MRN (Mre11-Rad50-NBS1) complex. Co-immunoprecipitation of endogenous LANA and MRN proteins in BC3 cells. Cells were lysed using TBS-T buffer and the cell lysate was incubated with benzonase. After centrifugation, supernatant was incubated overnight with anti-LANA or IgG-control beads. The precipitated complexes were analyzed for the presence of endogenous Rad50, Mre11 and NBS1 by SDS-PAGE and immunoblotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006335), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - ICC/IF analysis of Nbs1 in HeLa cells with Nbs1 Antibody [NB100-143]. Image from verified customer review.

Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-143] - FFPE human testis using Nbs1 antibody [NB100-143] at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Western Blot: Nbs1 Antibody [NB100-143] - Analysis of HeLa whole cell lysate [NB800-PC1] using rabbit polyclonal NBS1 antibody [NB100-143]. Observed Molecular Weight at ~95 kDa.

<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody [NB100-143] - Detection of NBS1 in Human Bone Osteosarcoma Epithelial Cells (U2OS whole cell lysate) using Nbs1 Antibody [NB100-143] at a dilution of 1:1000. Specificity was confirmed using a siRNA anti-NBS1 (targeting sequence: GGAAGAAACGUGAACUCAA). Image provided by Sebastien Britton of the Institute of Pharmacology and Structural Biology.

Western Blot: Nbs1 Antibody [NB100-143] - Western Blot with Nbs1 Antibody [NB100-143]. TIG-1 human primary fibroblasts, whole cell lysate (30 ug). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - Staining of HeLa cells using Nbs1 Antibody [NB100-143].

<b>Genetic Strategies Validation. </b>Knockdown Validated: Nbs1 Antibody [NB100-143] - TOPBP1 recruitment in response to rDNA breaks. TOPBP1 localization 2 h after I-Ppo1 transfection in NBS1-delta-N cells and NBS1-delta-N cells complemented with wild-type and mutant NBS1-mNG (percentage of cells with > 2 TOPBP1 caps are indicated; one of two experiments is shown). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31913317/) licensed under a CC-BY license.

HPV16 E2 partially colocalizes with the MRN complex proteins Mre11 and Nbs1. C33a cells were transfected with an HPV16 E2 expression plasmid, fixed in ice-cold methanol, and stained with an E2-specific antibody (green). DNA was stained with Hoechst 33342 (blue). (A) Endogenous Nbs1 was detected with an Nbs1-specific antibody (red/gray). (B) Endogenous Mre11 was detected with an Mre11-specific rabbit antibody (red/gray). Bar, 5 μm. Digital zoom is shown at the bottom.

TOPBP1 recruitment in response to rDNA breaks. b) TOPBP1 localization 2 h after I-Ppo1 transfection in NBS1deltaN cells and NBS1deltaN cells complemented with wild-type and mutant NBS1-mNG (percentage of cells with > 2 TOPBP1 caps are indicated; one of two experiments is shown). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31913317), licensed under a CC-BY licence.

ATM promotes cell migration and invasion independently of DNA DSB signaling in MDA-MB-231 cells.(B and C) Quantification of wound healing (B) and siRNA depletions (C) in (A). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26030852), licensed under a CC-BY licence.

KSHV LANA recruits MRN (Mre11-Rad50-NBS1) complex.(A) Co-immunoprecipitation of endogenous LANA and MRN proteins in BC3 cells. Cells were lysed using TBS-T buffer and the cell lysate was incubated with benzonase. After centrifugation, supernatant was incubated overnight with anti-LANA or IgG-control beads. The precipitated complexes were analyzed for the presence of endogenous Rad50, Mre11 and NBS1 by SDS-PAGE and immunoblotting. For the input, see Materials and methods. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006335), licensed under a CC-BY licence.

Replication centers of the integrated HPV recruit Mre11-Nbs1-Rad50 complex and Ku70/80 heterodimer.HeLa cells were transfected and analyzed as described previously. Co-immunostaining of HPV18 E1 (Alexa Fluor 568, second column) and the following proteins are presented: Mre11, Nbs1, Rad50, and Ku70/80 proteins (Alexa Fluor 488, first column). The localizations of the E1 and the respective DNA repair proteins are also shown in the third column as a merged image and DAPI stained nuclei are presented in the fourth column. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19390600), licensed under a CC-BY licence.

TRIB3 suppresses the functions of the UBE3B:MYC axis to attenuate lymphomagenesis.a Occupancy heatmap of genome-wide MYC peaks in MYCWT and MYCK427R Raji cells in a window of ±10 kb surrounding the transcription start site (TSS). b Distribution of MYC tags in MYCWT and MYCK427A Raji cells. Binding is shown for a window of ±2 kb around MYC tags as reference coordinates. c GSEA shows global downregulation of MYC target genes in Ad-UBE3B versus Ad-CTRL Raji cells. d Metagene plots of global RNAPII occupancy at gene bodies in Raji cells with or without UBE3B overexpression. e Colony-forming capacity of Raji cells with or without UBE3B overexpression. The data are presented as the means ± S.E.M from three independent experiments. Statistical significance was determined by two-tailed Student’s t test. P value: 0.00047. f Relative cell viabilities of Raji cells with overexpression of the indicated genes for the indicated times. The colors represent different time points; the diameter indicates the relative cell viability. The data are presented as the means ± S.E.M from three independent experiments. g Schematic strategy for studying BCL development in MycEμ mice with the indicated infection of Ad-GFP, Ad-Ube3b, Ad-Trib3, or Ad-Ube3b plus Ad-Trib3 in vivo. h Data are the spleen weights of MycEμ mice (n = 8 per group) with overexpression of the indicated genes. Data are represented as means ± SEM. Statistical significance was determined by two-tailed Student’s t test. P values: 2.077 × 10−5, 0.0009. i Data are representative LNs in MycEμ mice overexpressing Ad-GFP, Ad-Ube3b, Ad-Trib3, or Ad-Ube3b plus Ad-Trib3. Scale bar, 0.5 cm. j Cell proliferation was determined by Ki67 staining in the spleens of the indicated mice (n = 6 per group). Data are representative images of Ki67 staining and statistical analyses of Ki67-positive cells. Data are represented as means ± SEM. Statistical significance was determined by two-tailed Student’s t test. P values: 1.352 × 10−12, 3.674 × 10−8. Scale bar, 50 μm. k Approaches to defining the self-renewal ability of mouse LCs from the indicated mice (5 months old). l Colony-forming ability of BCL cells isolated from MycEμ mice (n = 6 per group) overexpressing Ad-GFP, Ad-Ube3b, Ad-Trib3, or Ad-Ube3b plus Ad-Trib3. Data are represented as means ± SEM. Statistical significance was determined by two-tailed Student’s t test. P values: 4.318 × 10−5, 0.0001. m The tumorigenicity of BCL cells from the indicated mice was compared by limiting dilution transplantation into NSG mice; n = 8 mice per group. n Schematic strategy for studying BCL development in MycEμCD19CreTrib3F/+ mice infected with or without lentivirus Ube3bCas9. o Kaplan–Meier survival curves for MycEμCD19CreTrib3F/+ mice infected with or without lentivirus Ube3bCas9 (n = 8 per group). Statistical difference was determined by two-sided log-rank test. P value: 0.0002. p Expression of MYC in the spleens of MycEμCD19CreTrib3F/+ mice (5 months old) infected with or without lentivirus Ube3bCas9. The data are presented as presentative from three independent experiments. q Colony-forming ability of BCL cells isolated from the LNs of the indicated mice (n = 6 per group). The data are representative or the means ± SEM of three assays. Statistical significance was determined by two-tailed Student’s t test. P value: 5.417 × 10−5, 0.0042. The MycEμCreCD19Trib3F/+ mice are heterozygous for the MycEμ allele. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33298911), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$The BNIP3 C-terminus can be phosphorylated.(A) Western blot detection of immunoprecipitated BNIP3 using an alpha -PKA substrate antibody specific to the phosphorylated RRXS/T sequence, located at the BNIP3 C-terminus and adjacent to the transmembrane (TM) domain. Results shown for 4 cell types: (left to right) HEK 293 cells expressing exogenous BNIP3 (dimer, 60 kD), and A549, MDA-MD-231, and AU565 cells expressing endogenous BNIP3 (monomer, 30kD). Lane 1 of each Western blot contains the whole cell lysate (WCL). (B) LC-MS/MS analysis of BNIP3 phosphorylation in HEK 293 cells with normal or elevated cAMP (8-Bromo-cAMP), showing peptide coverage (gray) and phosphorylation sites (red). The TM domain is underlined. (C) Table of BNIP3 phosphopeptides identified by LC-MS/MS, showing the percent probability, ion charge, actual and observed masses, and mass error (Da and ppm) for each peptide. Peptides shown are from analysis of BNIP3 purified from HEK 293 cells with elevated cAMP levels. (D) Schematic of the BNIP3 protein sequence, showing each C-terminal mutation representing phosphomimetic or nonphosphorylated BNIP3. (E) Expression of BNIP3 phosphomutants from stable doxycycline-inducible HEK 293 Tet On cells, treated with doxycycline (Dox) for 48 hr. (F) Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. (G) Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the alkaline-resistant mitochondrial pellet from cells expressing each BNIP3 phosphomutant. All blots are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26102349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$TAZ induces Tfam protein expression at the translational level.a The expression of mitochondrial biogenic factors, including Tfam, Ppargc1a (Pgc1 alpha ), Nrf1, and Nrf2, was analysed using quantitative reverse transcription (qRT)-PCR in the gastrocnemius muscle of wild-type (WT) and muscle-specific TAZ knockout (mKO) mice; n = 6 for each condition (Tfam; *p = 0.0498, Nrf1; *p = 0.0467). b Protein was analysed via immunoblotting to detect TAZ and the indicated mitochondrial biogenic factors in (a). Alpha-tubulin was used as the loading control. c RNA was isolated from the control (Con) and TAZ knockdown (KD) C2C12 myotubes, and the expression of the indicated mitochondrial biogenic factors was analysed using qRT-PCR. The experiments were performed in triplicate (Tfam; *p = 0.04). d Protein from the Con and TAZ KD C2C12 myotubes was assessed via immunoblotting to detect TAZ and mitochondrial biogenic factors. Beta-actin was used as the loading control. e RNA was isolated from WT, TAZ-knockout (KO), and TAZ-rescued (T) mouse embryonic fibroblasts (MEF), and the indicated mitochondrial biogenic factors were analysed via qRT-PCR. f Protein from WT, KO, and T MEF was assessed via immunoblotting to detect TAZ and the indicated mitochondrial biogenic factors. Beta-actin was used as the loading control. g Protein was isolated from the gastrocnemius muscle of WT and mKO mice and analysed via immunoblotting to detect TAZ, YAP, and proteins involved in the translation of both phosphorylated and total forms. Alpha-tubulin was used as the loading control. h Protein obtained from the Con and TAZ KD C2C12 myotubes was assessed via immunoblotting to observe TAZ and proteins involved in translation. Beta-actin was used as the loading control. i Protein was isolated from WT, KO, and T MEF and analysed via immunoblotting to detect TAZ and proteins involved in translation. Vinculin was used as the loading control. j The polysome profile of cells described in panel i was evaluated continuously by measuring absorbance at 254 nm. The 40S, 60S, 80S, and polysome fractions are denoted by the corresponding peaks. k The distribution of Tfam, Atp5d, and Gapdh mRNA from cells described in (j) across a density gradient was analysed using reverse transcription (RT)-PCR. bp = base pairs. For a, b, g 8 to 10-week-old mice were used. Data are presented as mean ± SEM for (a), mean ± SD for (c, e). Statistical significance was analysed via two-sided t-test for (a) and one-sided t-test for (c). One-way ANOVA with Tukey’s multiple comparison test was used for (e). Representative data was shown and experiments were performed at least twice with similar results for (b, d, f–i, k). Source data are provided as a Source data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35115527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

mTOR complex protein expressions and rictor phosphorylation at Ser1235.Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin and then stimulated with 10 nM IGF-1 in presence or absence of 10 nM E2 for 24 h. A, shown are representative western blots for mTOR, rictor and raptor and B,C,D, quantitative analysis with mean ± SEM of fold stimulation by IGF-1 of at least 3 independently performed experiments (B,C,D). * p < 0.05, **p < 0.0095. Exposure to rapamycin lead to downregulation of mTOR and rictor, which were more pronounced under culture conditions without E2. E, Western blots for GSK3 beta -pS9, GSK3 beta , rictor pS1235 and rictor and F,G,H,I quantitative analysis of at least 3 independently performed experiments indicate that rictor phosphorylation at S1235 by GSK-3 beta which has been reported to interfere with Akt-substrate binding to mTORC2, thereby downregulating mTORC2 activity. IGF-1 induced strong phosphorylation of GSK-3 beta at Ser9 in the absence and presence of E2, however, rapamycin pretreatment only reduced this increased phosphorylation in E2 co-treated cardiomyocytes, indicating increased activity of GSK-3 beta . This higher activity was not associated with increased phosphorylation of rictor at S1235. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Ha, Ma, Pm

Species Glossary

Reactivity: Hu, Mu, Ha, Ma, Pm

Applications:

, , , , , , , , , , , ,

Applications: , , , , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-221

Nbs1 Antibody (1D7)

(3 Reviews)

(21 Publications)

0.1 ml

NB100-221

Knockdown Validated: Nbs1 Antibody (1D7) [NB100-221] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (1D7) [NB100-221] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody [NBP2-19382] was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: Nbs1 Antibody (1D7) [NB100-221] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nbs1 Antibody (1D7) [NB100-221] diluted at1:500. The HRP-conjugated anti-mouse IgG antibody [NBP2-19382] was used to detect the primary antibody. Observed Molecular Weight at ~95 kDa.

Western Blot: Nbs1 Antibody (1D7) [NB100-221] - Sample (30 ug of whole cell lysate). A: HepG2. 7.5% SDS PAGE. Nbs1 Antibody (1D7) [NB100-221] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody. Observed Molecular Weight at ~97 kDa.

Immunocytochemistry/Immunofluorescence: Nbs1 Antibody (1D7) [NB100-221] - Immunofluorescence analysis of HeLa, using Nbs1 Antibody (1D7) [NB100-221] at 1:100 dilution.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-06609

Nbs1 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-06609

Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06609] - Staining of paraffin-embedded mouse bone marrow using Nbs1 Antibody [NBP1-06609].

Immunohistochemistry: Nbs1 Antibody [NBP1-06609] - Immunohistochemical staining of NBS1 proteins. Representative examples of typical nuclear staining of NBS1(c) scored as high expression in tumor cells. Correspondingly, examples scored as low expression (f) is shown (40x magnification). Image collected and cropped by CiteAb from the following publication (bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4776-9) licensed under a CC-BY license.

Western Blot: Nbs1 Antibody [NBP1-06609] - Analysis of Nbs1 in NIH/3T3 (Lane 4) and HeLa whole cell extract (Lane 5) with Nbs1 Antibody [NBP1-06609]. Observed molecular weight at ~99 kDa.

Immunohistochemical staining of MRE11, RAD50 and NBS1 proteins. Staining for each protein was scored as high or low as described in the Methods section. Representative examples of typical nuclear staining of MRE11 (a), RAD50 (b), and NBS1(c) scored as high expression in tumor cells. Correspondingly, examples of those scored as low expression for MRE11 (d), RAD50 (e), and NBS1 (f) are shown (40× magnification)

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-06609AF350
Alexa Fluor 405	NBP1-06609AF405
Alexa Fluor 488	NBP1-06609AF488
Alexa Fluor 532	NBP1-06609AF532
Alexa Fluor 594	NBP1-06609AF594
Alexa Fluor 647	NBP1-06609AF647
Alexa Fluor 700	NBP1-06609AF700
Alexa Fluor 750	NBP1-06609AF750
Allophycocyanin	NBP1-06609APC
Biotin	NBP1-06609B
DyLight 350	NBP1-06609UV
DyLight 405	NBP1-06609V
DyLight 488	NBP1-06609G
DyLight 550	NBP1-06609R
DyLight 594	NBP1-06609DL594
DyLight 650	NBP1-06609C
DyLight 680	NBP1-06609FR
DyLight 755	NBP1-06609IR
FITC	NBP1-06609F
HRP	NBP1-06609H
Janelia Fluor 549	NBP1-06609JF549
Janelia Fluor 646	NBP1-06609JF646
PE	NBP1-06609PE
PerCP	NBP1-06609PCP
mFluor Violet 450 SE	NBP1-06609MFV450
mFluor Violet 500 SE	NBP1-06609MFV500
mFluor Violet 610 SE	NBP1-06609MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-06609SS

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NBP1-06705

Nbs1 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-06705

Western Blot: Nbs1 Antibody [NBP1-06705] - Western Blot analysis of Nbs1 on NIH/3T3 (Lane 6) and HeLa whole cell extract (Lane 7) with Nbs1 Antibody [NBP1-06705]. Observed molecular weight at ~99 kDa.

Immunohistochemistry-Paraffin: Nbs1 Antibody [NBP1-06705] - Staining of paraffin-embedded mouse skin with Nbs1 Antibody [NBP1-06705].

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-06705AF350
Alexa Fluor 405	NBP1-06705AF405
Alexa Fluor 488	NBP1-06705AF488
Alexa Fluor 532	NBP1-06705AF532
Alexa Fluor 594	NBP1-06705AF594
Alexa Fluor 647	NBP1-06705AF647
Alexa Fluor 700	NBP1-06705AF700
Alexa Fluor 750	NBP1-06705AF750
Allophycocyanin	NBP1-06705APC
Biotin	NBP1-06705B
DyLight 350	NBP1-06705UV
DyLight 405	NBP1-06705V
DyLight 488	NBP1-06705G
DyLight 550	NBP1-06705R
DyLight 594	NBP1-06705DL594
DyLight 650	NBP1-06705C
DyLight 680	NBP1-06705FR
DyLight 755	NBP1-06705IR
FITC	NBP1-06705F
HRP	NBP1-06705H
Janelia Fluor 549	NBP1-06705JF549
Janelia Fluor 646	NBP1-06705JF646
PE	NBP1-06705PE
PerCP	NBP1-06705PCP
mFluor Violet 450 SE	NBP1-06705MFV450
mFluor Violet 500 SE	NBP1-06705MFV500
mFluor Violet 610 SE	NBP1-06705MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-06705SS

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MAB1573

Nbs1 Antibody (206919) [Unconjugated]

(1 Publication)

100 μg

MAB1573

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line,. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Nbs1 Monoclonal Antibody (Catalog # MAB1573) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nbs1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Nbs1 Monoclonal Antibody (Catalog # MAB1573) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1573-SP

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NB100-60654

Nbs1 Antibody

(2 Publications)

0.1 ml

NB100-60654

Western Blot: Nbs1 Antibody [NB100-60654] - Detection of human Nbs1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibodies NB100-60653 and NB100-60654, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).

<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-60654] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60654] used at a dilution of 1:200 (1ug/ml). Detection: DAB.

Reactivity:

Hu, Mu, Pm-Or

Species Glossary

Reactivity: Hu, Mu, Pm-Or

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB15731

Nbs1 Antibody (206911)

100 μg

MAB15731

Nbs1 was immunoprecipitated from 500 µg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2 µg Mouse Anti-Human/Mouse Nbs1 Mono-clonal Antibody (Catalog # MAB15731) for 1 hour on ice. Nbs1-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Nbs1 was detected by Western blot using 1 µg/mL Human/Mouse/Rat Nbs1 Mono-clonal Antibody (Catalog # MAB1573). View our recommended buffer recipes for immunoprecipitation.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB15731-SP

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NB100-60648

Nbs1 Antibody

(1 Publication)

0.1 ml

NB100-60648

Western Blot: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] (lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Observed Molecular Weight at ~95 kDa.

<b>Independent Antibodies Validation. </b>Immunoprecipitation: Nbs1 Antibody [NB100-60648] - Detection of mouse Nbs1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from L1210 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nbs1 antibody [NB100-60648] used for IP at 3 ug per reaction. Nbs1 was also immunoprecipitated by rabbit anti-Nbs1 antibody from Company B. For blotting immunoprecipitated NBS1, NB100-60648 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Band detected at ~95 kDa.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-06609AF647

Nbs1 Antibody [Alexa Fluor® 647]

0.1 ml

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Alexa Fluor 647

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-06609AF350
Alexa Fluor 405	NBP1-06609AF405
Alexa Fluor 488	NBP1-06609AF488
Alexa Fluor 532	NBP1-06609AF532
Alexa Fluor 594	NBP1-06609AF594
Alexa Fluor 700	NBP1-06609AF700
Alexa Fluor 750	NBP1-06609AF750
Allophycocyanin	NBP1-06609APC
Biotin	NBP1-06609B
DyLight 350	NBP1-06609UV
DyLight 405	NBP1-06609V
DyLight 488	NBP1-06609G
DyLight 550	NBP1-06609R
DyLight 594	NBP1-06609DL594
DyLight 650	NBP1-06609C
DyLight 680	NBP1-06609FR
DyLight 755	NBP1-06609IR
FITC	NBP1-06609F
HRP	NBP1-06609H
Janelia Fluor 549	NBP1-06609JF549
Janelia Fluor 646	NBP1-06609JF646
PE	NBP1-06609PE
PerCP	NBP1-06609PCP
mFluor Violet 450 SE	NBP1-06609MFV450
mFluor Violet 500 SE	NBP1-06609MFV500
mFluor Violet 610 SE	NBP1-06609MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-06609
BSA Free	NBP1-06609SS

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