| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-235SS | |||
| Azide and BSA Free | NBP2-80597 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-59787SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23484SS | |||
| Azide and BSA Free | NBP2-81015 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56548SS | |||
| Azide Free | NBP2-33225 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-598SS | |||
| Azide and BSA Free | NBP2-80600 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80602 | |||
![Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody (NBP1-05987) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-05987-img0017.jpg "Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody (NBP1-05987) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-05987-img0019.jpg)
![Western Blot: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in transfected and native mouse cell lysate. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-05987-img0003.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Analysis of ZEB1 in human liver and spleen tissues. Image courtesy of anonymous customer review.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-05987-img0004.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-05987] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (NBP1-05987) used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-05987-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ZEB1 Antibody [NBP1-05987] - Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZEB1 antibody NBP1-05987 used for IP at 3 ug per reaction. ZEB1 was also immunoprecipitated by another rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1, NBP1-05987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunoprecipitation-NBP1-05987-img0013.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0031.jpg "<b>Biological Strategies Validation. </b>Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-BLIMP1 (3H2-E8) at 10 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0028.jpg)
![Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers. Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunohistochemistry-Paraffin-NB600-235-img0025.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) - BSA Free [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-BSA-Free-Flow-Cytometry-NB600-235-img0032.jpg)
![Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Western-Blot-NB600-235-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-BLIMP1/PRDM1 (3H2-E8) conjugated to DyLight 550 [NB600-235R] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Immunocytochemistry-Immunofluorescence-NB600-235-img0027.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0011.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0013.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0015.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0024.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0029.jpg)
![Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BLIMP1-PRDM1-Antibody-3H2-E8-Flow-Cytometry-NB600-235-img0030.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NB100-59787-img0018.jpg "Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.")
![Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-NB100-59787-img0017.jpg)
![Western Blot: PML Protein Antibody [NB100-59787] - Detection of Human PML on HeLa whole cell lysate using NB100-59787. PML was also immunoprecipitated by rabbit anti-PML antibodies NB100-59788 and another CDC16/APC6 Ab.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59787-img0016.jpg)
![Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. Image collected and cropped by Citeab from the following publication (HIF factors cooperate with PML-RAR +/- to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med (2014)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Western-Blot-NB100-59787-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PML-Protein-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-59787-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PML-Protein-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-59787-img0020.jpg)
![Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-PML used at a dilution of 1:500](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Immunohistochemistry-Paraffin-NB100-59787-img0015.jpg)
![Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Proximity-Ligation-Assay-NB100-59787-img0006.jpg)
![Proximity Ligation Assay: PML Protein Antibody [NB100-59787] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.](http://images.novusbio.com/fullsize/PML-Protein-Antibody-Proximity-Ligation-Assay-NB100-59787-img0014.jpg)
![Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - CAFs induced miR-33b downregulation and promoted the EMT phenotype in lung cancer cells. The protein expression levels of E-cadherin, Vimentin, ZEB1, Twist1 and Snail1 in the lung cancer cells co-cultured with CAFs were detected by immunoblotting, using GAPDH protein as the loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23082), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Western-Blot-NBP2-23484-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - CAFs induced miR-33b downregulation and promoted the EMT phenotype in lung cancer cells. The protein expression levels of E-cadherin, Vimentin, ZEB1, Twist1 and Snail1 in the lung cancer cells co-cultured with CAFs were detected by immunoblotting, using GAPDH protein as the loading control. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23082), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) [NBP2-23484] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Immunocytochemistry-Immunofluorescence-NBP2-23484-img0009.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8A6) [NBP2-23484] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Immunohistochemistry-NBP2-23484-img0006.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on U-87 cells with ZEB1 [2A8A6] Antibody NBP2-23484AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZEB1 Antibody (2A8A6) [NBP2-23484] - ZEB1 expression was assayed in lung adenocarcinoma cells after transfectionwith miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells. Image submitted by verified customer review.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Western-Blot-NBP2-23484-img0013.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8A6) [NBP2-23484] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Immunohistochemistry-NBP2-23484-img0004.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0007.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on with A549 cells with ZEB1 Antibody NBP2-23484PE and a a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0010.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8A6) [NBP2-23484] - An intracellular stain was performed on Jurkat with ZEB1 Antibody (2A8A6) NBP2-23484 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Flow-Cytometry-NBP2-23484-img0011.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Analysis in human cerebral cortex and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Analysis in human cerebral cortex and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody [NBP1-88845] - Mesenchymal-high cancers exhibit enhanced sensitivity to ferroptosis inducers. Representative cell lines showing enhanced sensitivity to GPx4 inhibitor ML162 correlates to low levels of E-cadherin, an epithelial marker. H1975 and A549: lung cancer; KLE and Ishikawa: endometrial cancer. Antibodies against E-cadherin, ZEB1, vimentin, and MTDH were used to detect protein extracts from non-small-cell lung cancer H1975 and A549, and endometrial cancer cell lines KLE and Ishikawa. Beta-Actin was detected as a loading control. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6746770/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Western-Blot-NBP1-88845-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody [NBP1-88845] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88845-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0020.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0008.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0011.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human cerebral cortex shows strong nuclear positivity in glial cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0015.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human glioma shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-Immunohistochemistry-Paraffin-NBP1-88845-img0017.jpg)
![Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-158-img0028.jpg "Western Blot: KAP1 Antibody [NB500-158] - Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: KAP1 Antibody [NB500-158] - FFPE sections of human breast carcinoma. KAP1 antibody Lot3 (left) and Lot2 (right) used at a dilution of 1:1000 (1 ug/mL). Detection: DAB.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunohistochemistry-NB500-158-img0022.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: KAP1 Antibody [NB500-158] - Detection of Human KAP1 by Western blot. Whole cell lysate from HeLa (5, 15 and 50 ug), and 293T (50 ug) cells. KAP1 antibody at 0.1 ug/mL. Detection by chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-158-img0024.jpg)
![Western Blot: KAP1 Antibody [NB500-158] - Hep3B human hepatocellular carcinoma cell line. 50 ug cell lysate per lane. 10% SDS-PAGE. KAP1 (TRIM28) antibody at 1:1000 dilution in 5% milk at 4C O/N. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KAP1-Antibody-Western-Blot-NB500-158-img0025.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 Antibody [NB500-158] - Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NB500-158 (lot NB500-158-3) used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-158-2) and rabbit anti-KAP-1 antibody NB500-159. For blotting immunoprecipitated KAP-1, NB500-158 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/KAP1-Antibody-Immunoprecipitation-NB500-158-img0026.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0016.jpg "Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.Lane 1: RAD18 transfected lysate(56.2 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - The antibody was used to detect RAD18 in U2os Cells. Anti-mouse 2nd antibody was used for detection (Red). DAPI was used for nuclear staining (Blue). This image was submitted via customer Review.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunofluorescence-H00056852-M01-img0018.jpg)
![Immunohistochemistry-Paraffin: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunohistochemistry-Paraffin-H00056852-M01-img0011.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # H00056852-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0012.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7. Analysis of RAD18 expression in PC-12.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0014.jpg)
![Western Blot: RAD18 Antibody (3H7) [H00056852-M01] - RAD18 monoclonal antibody (M01), clone 3H7 Analysis of RAD18 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Western-Blot-H00056852-M01-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: RAD18 Antibody (3H7) [H00056852-M01] - Analysis of monoclonal antibody to RAD18 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-Immunocytochemistry-Immunofluorescence-H00056852-M01-img0009.jpg)
![ELISA: RAD18 Antibody (3H7) [H00056852-M01] - Detection limit for recombinant GST tagged RAD18 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAD18-Antibody-3H7-ELISA-H00056852-M01-img0017.jpg)
![Western Blot: Livin Antibody (88C570) [NB100-56548] - detection of Livin in A) Livin transfected cell lysate and B) Molt4 cell lysate. A protein band of apprximate molecular weight of 31 kDa is detected using this antibody.](http://images.novusbio.com/fullsize/Livin-Antibody-88C570-Western-Blot-NB100-56548-img0002.jpg "Western Blot: Livin Antibody (88C570) [NB100-56548] - detection of Livin in A) Livin transfected cell lysate and B) Molt4 cell lysate. A protein band of apprximate molecular weight of 31 kDa is detected using this antibody.")
![TRAF6 Inhibitor Peptide Set [NBP2-26506] - The TRAF6 inhibitory peptide inhibits osteoclast ifferentiation: RAW264.7 cells were plated on 24-well plates and stimulated with RANKL and M-CSF and incubated in the presence of different concentrations of control peptide (PTD) or the TRAF6. The TRAF6 at the concentration above 30 uM suppressed osteoclast differentiation.](http://images.novusbio.com/fullsize/TRAF6-Inhibitor-Peptide-Set-NBP2-26506-img0002.jpg "TRAF6 Inhibitor Peptide Set [NBP2-26506] - The TRAF6 inhibitory peptide inhibits osteoclast ifferentiation: RAW264.7 cells were plated on 24-well plates and stimulated with RANKL and M-CSF and incubated in the presence of different concentrations of control peptide (PTD) or the TRAF6. The TRAF6 at the concentration above 30 uM suppressed osteoclast differentiation.")
![Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Western-Blot-NB100-598-img0006.jpg "Western Blot: BRCA1 Antibody (RAY) [NB100-598] - Analysis of BRCA1 on MCF7 lysate.")
![Immunocytochemistry/Immunofluorescence: BRCA1 Antibody (RAY) [NB100-598] - BRCA (RAY) antibody was tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Immunocytochemistry-Immunofluorescence-NB100-598-img0005.jpg)
![Flow Cytometry: BRCA1 Antibody (RAY) [NB100-598] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue) and isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-RAY-Flow-Cytometry-NB100-598-img0008.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0004.jpg "Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.")
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] NB100-600C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0006.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 in MCF-7 whole cell lysate using NB100-600. 2 minute ECL exposure.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0003.jpg)
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0005.jpg)
![Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RNF20-Antibody-Western-Blot-NB100-2242-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: RNF20 Antibody [NB100-2242] - Detection of Human and Mouse RNF20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF20 antibody NB100-2242 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: RNF20 Antibody [NB100-2242] - WB analysis of RNF20 in HeLa whole cell lysate. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/RNF20-Antibody-Western-Blot-NB100-2242-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: RNF20 Antibody [NB100-2242] - Detection of human RNF20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF20 antibody NB100-2242 (lot NB100-2242-2) used for IP at 6 ug per reaction. RNF20 was also immunoprecipitated by a previous lot of this antibody (NB100-2242-1) and rabbit anti-RNF20 antibody NB100-2243. For blotting immunoprecipitated RNF20, NB100-2242 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RNF20-Antibody-Immunoprecipitation-NB100-2242-img0007.jpg)