| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58358SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27399 | |||
| Azide and BSA Free | NBP2-27399SS | |||
| BSA Free | NB100-56749SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36429-0.025mg | |||
| Azide and BSA Free | NBP2-81009 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-77477SS | |||
| Azide and BSA Free | NBP2-80624 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43359-0.05mg | |||
| BSA Free | NBP1-43359-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36428SS | |||
| Azide and BSA Free | NBP2-81008 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22117SS | |||
| Azide and BSA Free | NBP2-81014 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77680SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44306SS | |||
| Azide and BSA Free | NBP2-44309SS | |||
| Azide and BSA Free | NBP2-44309 | |||
![Immunocytochemistry/Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with YAP1 Antibody (NB110-58358) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-58358-img0014.jpg "Immunocytochemistry/Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with YAP1 Antibody (NB110-58358) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![<b>Biological Strategies Validation. </b>Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - LW6 attenuates the accumulation of cellular YAP1 and its nuclear location. After treating PANC-1 cells with LW6 for 12 hours, LW6 decreased the accumulation of YAP1 when compared to Sham treated cells. n =5 per group. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31897243/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/YAP1 Antibody - BSA Free-Western Blot-NB110-58358-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: YAP1 Antibody - BSA Free [NB110-58358] - YAP/TAZ signaling is not activated by loss of TRAF3. NT, NF2 KO1, and TRAF3 KO1 cells stained for YAP1 and DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33185187/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-58358-img0015.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody - BSA Free [NB110-58358] - YAP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human YAP1 polyclonal Antibody (Catalog # NB110-58358) at 1:200 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclear and cytoplasm in trophoblast cells.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB110-58358-img0010.jpg)
![Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Analysis in transfected HEK 293 cell lysate using YAP1 antibody. Observed molecular weight 75 kDa.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Western-Blot-NB110-58358-img0003.jpg)
![Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - Consequences of ORP5 and ORP8 knockdown on downstream MAPK and PI3K/AKT signaling. Protein from MOH parental, single and double ORP knockdowns as well as cells transfected with empty vector control (pLKO.1) were harvested, and 20 ug was subjected to SDS-PAGE and used for Western blotting. Image collected and cropped by CiteAb from the following publication (//www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900431), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Western-Blot-NB110-58358-img0012.jpg)
![Western Blot: YAP1 Antibody - BSA Free [NB110-58358] - YAP1 Antibody [NB110-58358] - Actomyosin activity inhibits beta-catenin- and YAP-driven proliferation of confluent keratinocytes. Effects of cell density and actomyosin activity on YAP phosphorylation. HaCaT cells cultured for 40 h under the sparse and confluent conditions were treated with 100 uM blebbistatin (Blebb) or DMSO (for control) for 6 h, and then lysed and immunoblotted for Ser127-phosphorylated YAP (pYAP), YAP, beta-catenin and actin. Similar results were obtained in two independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep46326), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Western-Blot-NB110-58358-img0011.jpg)
![Simple Western: YAP1 Antibody - BSA Free [NB110-58358] - Simple Western lane view shows a specific band for YAP1 in 0.1 mg/ml of HeLa lysate. Observed molecular weight is 75 kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Simple-Western-NB110-58358-img0007.jpg)
![Knockout Validated: YAP1 Antibody - BSA Free [NB110-58358] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human YAP1 Polyclonal Antibody (Catalog # NB110-58358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Knockout-Validated-NB110-58358-img0008.jpg)
![Knockout Validated: YAP1 Antibody - BSA Free [NB110-58358] - Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 50 ug/mL of Rabbit Anti-YAP1 Polyclonal Antibody (Catalog # NB110-58358). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/YAP1-Antibody---BSA-Free-Knockout-Validated-NB110-58358-img0009.jpg)
![Knockdown Validated: YAP1 Antibody (2F12) - Isoform 5 [H00010413-M01] - YAP1 Antibody (2F12) [H00010413-M01] - JNK phosphorylation of YAP enhances the stabilization of delta-Np63alpha by direct binding. H357 cells were transfected with control siRNA (siCTL) or YAP siRNA (siYAP) and 72 h later the cells were harvested for IP of endogenous p63. p63 IP eluates and input lysates were analyzed by Western blotting using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis20107) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/YAP1-Antibody-2F12-Knockdown-Validated-H00010413-M01-img0016.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: YAP1 Antibody (2F12) - Isoform 5 [H00010413-M01] - YAP1 Antibody (2F12) [H00010413-M01] - JNK phosphorylation of YAP enhances the stabilization of delta-Np63alpha by direct binding. H357 cells were transfected with control siRNA (siCTL) or YAP siRNA (siYAP) and 72 h later the cells were harvested for IP of endogenous p63. p63 IP eluates and input lysates were analyzed by Western blotting using the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis20107) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of YAP1 Antibody (2F12) Isoform 5 expression in Hela S3 N cells. Observed molecular weight 75 kDa.](http://images.novusbio.com/fullsize/YAP1-Antibody-2F12-Western-Blot-H00010413-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on HeLa cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/YAP1-Antibody-2F12-Immunocytochemistry-Immunofluorescence-H00010413-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of monoclonal antibody to YAP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/YAP1-Antibody-2F12-Immunohistochemistry-Paraffin-H00010413-M01-img0011.jpg)
![Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - JNK specifically phosphorylates YAP in vivo. HaCaT, U2OS, MCF7 and 293T cell lines were treated with anisomycin or DMSO (control) for 60 min before harvesting. YAP band shift was analyzed as described before and lysates were analyzed by Western blotting with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis20107) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/YAP1-Antibody-2F12-Western-Blot-H00010413-M01-img0017.jpg)
![Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Immunogen detection using YAP1 antibody (2F12) Isoform 5, observered MW 37.73KDa) .](http://images.novusbio.com/fullsize/YAP1-Antibody-2F12-Western-Blot-H00010413-M01-img0019.jpg)
![Western Blot: YAP1 Antibody (2F12) [H00010413-M01] - Analysis of YAP1 Antibody (2F12) Isoform 5 in transfected 293T cell line. Lane 1: YAP1 transfected lysate (TMW: 54.5 KDa, observed MW 75 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/YAP1-Antibody-2F12-Western-Blot-H00010413-M01-img0018.jpg)
![Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY S100A9 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A9.](http://images.novusbio.com/fullsize/S100A9-Antibody-Western-Blot-NB110-89726-img0007.jpg "Western Blot: S100A9 Antibody [NB110-89726] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY S100A9 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A9.")
![Immunocytochemistry/Immunofluorescence: S100A9 Antibody [NB110-89726] - S100A9 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/S100A9-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89726-img0008.jpg)
![Immunohistochemistry-Paraffin: S100A9 Antibody [NB110-89726] - Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200x magnification) of S100a9, in normal control, IgG Ab, and anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 um. Staining scores were determined by semi-quantitative optical analysis. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2017.01774/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S100A9-Antibody-Immunohistochemistry-Paraffin-NB110-89726-img0010.jpg)
![Western Blot: S100A9 Antibody [NB110-89726] - Analysis of S100A9 Antibody in DMSO treated HL60 whole cell lysates.](http://images.novusbio.com/fullsize/S100A9-Antibody-Western-Blot-NB110-89726-img0006.jpg)
![Simple Western: S100A9 Antibody [NB110-89726] - Simple Western lane view shows a specific band for S100A9 in 0.5 mg/ml of Human PBMC's lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/S100A9-Antibody-Simple-Western-NB110-89726-img0009.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0014.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0015.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0009.jpg)
![Immunohistochemistry-Frozen: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Rat mammary carcinoma tissue section stained with AKT1 [p Ser473] Antibody (104A282). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Frozen-NB100-56749-img0016.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0010.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Western-Blot-NBP2-36429-img0018.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.")
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunocytochemistry-Immunofluorescence-NBP2-36429-img0012.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0017.jpg)
![Flow (Cell Surface): VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Flow-Cell-Surface-NBP2-36429-img0011.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of VEGF R2/KDR/Flk-1 protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0013.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm while the tumor stroma was negative for VEGF R2/KDR/Flk-1 immunopositivity.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0014.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0015.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-2C6-Immunohistochemistry-Paraffin-NBP2-36429-img0016.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0018.jpg "Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human HEK293 cells showing detection of Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane A: Nitrosylated-HEK293 (15uL). Lane B: HEK293 (15 ug). Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) diluted in 1.5% BSA and TBST for 1 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG: HRP at 1:4000 for 1 hour at RT with shaking . Predicted/Observed Size: Multiple Bands.")
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Backskin obtained from transgenic mice.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0021.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human A549 cells showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody (NB110-96877) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0016.jpg)
![Western Blot: Nitrotyrosine Antibody (39B6) [NB110-96877] - Western Blot analysis of Human Recombinant Protein showing detection of Multiple Bands Nitrotyrosineprotein using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Lane 1: MW Ladder. Lane 2: hASYN Monomer (3.84 ug). Lane 3: Nitrosylated hASYN (3.84 ug).. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000 for 2 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 2 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple Bands.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Western-Blot-NB110-96877-img0017.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0014.jpg)
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immuno-peroxidase (DAB) staining for nitrotyrosine (upper row) and immuno-alkaline phosphatase (ALP) staining for 8-OHdG (bottom row), hematoxylin counterstain. Representative small intestinal tumor histopathology of Category 1 (low-grade adenoma) in ApcMin/+ (left column) and ApcMin/+ Mieap-/- (second from left column) mice, Category 2 (high-grade adenoma, second from right column) and Category 3 (adenocarcinoma, right column) in the ApcMin/+ Mieap-/- mice. Scale bars: 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep12472), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-Paraffin-NB110-96877-img0015.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0019.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: liver tissue . Species: Rat. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0020.jpg)
![Immunohistochemistry: Nitrotyrosine Antibody (39B6) [NB110-96877] - Immunohistochemistry analysis using Mouse Anti-NitrotyrosineMonoclonal Antibody, Clone 39B6 (NB110-96877). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-NitrotyrosineMonoclonal Antibody (NB110-96877) at 1:25000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-39B6-Immunohistochemistry-NB110-96877-img0022.jpg)
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0003.jpg "Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Detection of CD117/c-kit in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse B220/CD45R (Catalog # FAB1217P) and Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # NB100-77477, top panel) or isotype control antibody (Catalog # MAB0061, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).")
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - Identification of CD117/c-kit expressing bone marrow cells.Double labeling of mouse bone marrow cells with anti-CD45 antibody and rabbit anti-CD117/c-kit antibody 2B8. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0071798) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0004.jpg)
![Flow Cytometry: CD117/c-kit Antibody (2B8) [NB100-77477] - C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-2B8-Flow-Cytometry-NB100-77477-img0002.jpg)
![Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Immunohistochemistry-Whole-Mount-NBP1-43359-img0003.jpg "Immunohistochemistry Whole-Mount: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Fluorescent images from representative ileum slices show putative neuronal cells and interstitial cells of Cajal. Immunoreactive c-Kit was localized by using the ACK2 antibody in live tissue after 48 h ex vivo. Scale bars are 25 um. Image collected and cropped by CiteAb from the following publication (//www.physiology.org/doi/10.1152/ajpgi.00299.2015) licensed under a CC-BY license.")
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of Allophycocyanin conjugate of NBP1-43359. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b kappa Isotype Control APC (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) APC (right). Total viable c](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0001.jpg)
![Flow Cytometry: CD117/c-kit Antibody (ACK2) [NBP1-43359] - Analysis of PE/Cy5 conjugate of NBP1-43359. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.03 ug of Rat IgG2b Isotype Control PE-Cy5 (left) or 0.03 ug of Anti-Mouse CD117 (c-Kit) PE-Cy5 (right). Total viable cells were](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-ACK2-Flow-Cytometry-NBP1-43359-img0002.jpg)
![Western Blot: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of VEGF R2/KDR/Flk-1 (1B6) in purified human recombinant protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Western-Blot-NBP2-36428-img0014.jpg "Western Blot: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of VEGF R2/KDR/Flk-1 (1B6) in purified human recombinant protein.")
![Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Human brain endothelial cells stained for VEGFR2 with antibody at concentration of 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VEGFR2-KDR-Flk-1-Antibody-1B6-Immunocytochemistry-Immunofluorescence-NBP2-36428-img0016.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE transverse section of normal human skeletal muscle with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0013.jpg)
![Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - HepG2 cells were stained (surface) with VEGF R2/KDR/Flk-1 antibody (clone: 2B5.1B6, orange) or isotype control (mouse IgG1, blue) and positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Flow-Cytometry-NBP2-36428-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Detection in HUVEC cells at 1:50](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunocytochemistry-Immunofluorescence-NBP2-36428-img0009.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of human normal kidney with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of protein.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0010.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of human liver cancer with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells showed a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0011.jpg)
![Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.](http://images.novusbio.com/fullsize/VEGF-R2-KDR-Flk-1-Antibody-1B6-Immunohistochemistry-Paraffin-NBP2-36428-img0012.jpg)
![Knockout Validated: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # NBP2-22117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Knockout-Validated-NBP2-22117-img0010.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human YAP1 Monoclonal Antibody (Catalog # NBP2-22117) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific band was detected for YAP1 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: YAP1 Antibody (1A12) - BSA Free [NBP2-22117] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with YAP1 Antibody [1A12] (NBP2-22117) at 1 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-22117-img0012.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded prostate cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Immunohistochemistry-Paraffin-NBP2-22117-img0009.jpg)
![Western Blot: YAP1 Antibody (1A12) [NBP2-22117] - Analysis using YAP1 mouse mAb against Hela (1), C6 (2) and Cos7 (3) cell lysate. Dilution 1:500 - 1:2000.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Western-Blot-NBP2-22117-img0008.jpg)
![Immunohistochemistry-Paraffin: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of paraffin-embedded rectum cancer tissues using YAP1 mouse mAb with DAB staining. 1:200 - 1:1000](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Immunohistochemistry-Paraffin-NBP2-22117-img0006.jpg)
![Flow Cytometry: YAP1 Antibody (1A12) [NBP2-22117] - Analysis of Hela cells using YAP1 mouse mAb (green) and negative control (red). Dilution 1:200 - 1:400.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Flow-Cytometry-NBP2-22117-img0004.jpg)
![ELISA: YAP1 Antibody (1A12) [NBP2-22117] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng). Dilution 1:10000.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-ELISA-NBP2-22117-img0001.jpg)
![Knockout Validated: YAP1 Antibody (1A12) [NBP2-22117] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and YAP1 knockout (KO) HeLa cell line. A specific band was detected for YAP1 at approximately 81 kDa (as indicated) using 20 ug/mL of mouse Anti-YAP1 Monoclonal Antibody (Catalog # NBP2-22117). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/YAP1-Antibody-1A12-Knockout-Validated-NBP2-22117-img0011.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0009.jpg "Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGFR1 in: 1. HeLa, 2. NIH/3T3, 3. SK-BR-3 and 4. COS7.")
![Immunocytochemistry/Immunofluorescence: IGF-I R Antibody [NBP1-77680] - IGF1 receptor (EC) antibody was tested in A431 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77680-img0008.jpg)
![Immunohistochemistry-Paraffin: IGF-I R Antibody [NBP1-77680] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney cancer xenograft using 1:200 dilution of IFG-I R antibody (NBP1-77680). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Immunohistochemistry-Paraffin-NBP1-77680-img0010.jpg)
![Western Blot: IGF-I R Antibody [NBP1-77680] - Analysis of IGF-I R in human pancreatic cancer cell lines (MiaPaCa-2, AsPC-1, and BxPC-3) using anti-IGF-I R antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IGF-I-R-Antibody-Western-Blot-NBP1-77680-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HGFR/c-MET Antibody [1G7NB] (NBP2-44306) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Immunocytochemistry-Immunofluorescence-NBP2-44306-img0007.jpg "Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HGFR/c-MET Antibody [1G7NB] (NBP2-44306) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - Analysis of HGF R/c-Met (1G7NB) in human liver cancer.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Immunohistochemistry-Paraffin-NBP2-44306-img0001.jpg)
![Flow Cytometry: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Flow-Cytometry-NBP2-44306-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Immunocytochemistry-Immunofluorescence-NBP2-44306-img0003.jpg)