| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61137 | |||
| Azide and BSA Free | NBP2-80956 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Non-Recombinant Monoclonal | NB600-1159SS | |||
| Azide Free | NBP2-33250 | |||
| Non-Recombinant Monoclonal | NB600-1159 | |||
| BSA Free | NBP3-07012SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-39113SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80868 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74682SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-19051SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80877 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-156SS | |||
| Azide and BSA Free | NBP2-80857 | |||
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details are included in Methods. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-261), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Western-Blot-NB300-146-img0022.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details are included in Methods. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-261), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the expected respective apical and basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0023.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial smooth muscle.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunohistochemistry-NB300-146-img0012.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on NIH3T3 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0027.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Western-Blot-NB300-146-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the expected respective apical and basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The expression of Na+, K+-ATPase using anti-alpha1 antibody was observed mostly at the basolateral membrane. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0020.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NB300-146 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0016.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NBP2-61137AF647 (Blue) and a matched isotype control (Orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0017.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with - Purified NBP2-61137 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Purified form of this antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0018.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on RCC4 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0025.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on SK-MEL-28 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0026.jpg)
![Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The expression of Na+, K+-ATPase using anti-alpha1 antibody was observed mostly at the basolateral membrane. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-NB300-146-img0024.jpg)
![Western Blot: Noxa Antibody (114C307.1R) [NBP3-07012] - Doc counteracts the ABT-737-mediated increase in Mcl-1 protein. Western blot showing treatment of LNCaP and PC3 cells with 1 uM ABT-737. In LNCaP and PC3, there are few difference in Noxa. Image collected and cropped by CiteAb from the following publication (//peerj.com/articles/144), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1R-Western-Blot-NBP3-07012-img0003.jpg "Western Blot: Noxa Antibody (114C307.1R) [NBP3-07012] - Doc counteracts the ABT-737-mediated increase in Mcl-1 protein. Western blot showing treatment of LNCaP and PC3 cells with 1 uM ABT-737. In LNCaP and PC3, there are few difference in Noxa. Image collected and cropped by CiteAb from the following publication (//peerj.com/articles/144), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1R) [NBP3-07012] - ICC/IF analysis using the azide-free version of NB600-1159 at 1:10. HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1R-Immunocytochemistry-Immunofluorescence-NBP3-07012-img0004.jpg)
![Flow Cytometry: Noxa Antibody (114C307.1R) [NBP3-07012] - An intracellular stain was performed on Ntera2 cells with Noxa Antibody [114C307.1R] NBP3-07012 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1R-Flow-Cytometry-NBP3-07012-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Noxa Antibody (114C307.1R) [NBP3-07012] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Noxa Antibody (114C307.1R) NBP3-07012 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Noxa-Antibody-114C307-1R-Immunocytochemistry-Immunofluorescence-NBP3-07012-img0001.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: Glut1 Antibody [NB110-39113] - PrP-mediated increase in IC iron downregulates glucose transporters in the brain, neuroretina, and the liver: A similar evaluation of retinal lysates shows upregulation of Glut1 in PrP-/- relative to Tg40 PrP samples (lanes 1 & 3). Iron overloading downregulates Glut1 in Tg40 PrP samples relative to untreated controls, but has minimal effect on similarly treated PrP-/- samples (lanes 2 & 4). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-24786-1) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: Glut1 Antibody [NB110-39113] - Western blot of GLUT1 on mouse kidney membrane protein (lane A) and rat kidney membrane protein (lane B).](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0006.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NB110-39113] - Immunohistochemical analysis of FFPE tissue section of human placenta using 1:200 dilution of Glut1 antibody. The staining was developed using HRP-DAB detection method and the sections were further counterstained with hematoxylin. This antibody generated a specific strong membrane cytoplasmic staining of Glut1 primarily in the syncytiotrophoblast layers of various villi and in the red blood cells (RBCs). Cytotrophoblasts showed a very weak expression of this protein.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NB110-39113-img0011.jpg)
![Flow Cytometry: Glut1 Antibody [NB110-39113] - An intracellular stain was performed on HepG2 with NB110-39113 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/Glut1-Antibody-Flow-Cytometry-NB110-39113-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NB110-39113] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-39113-img0013.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - Analysis of HeLa lysates using NB110-39113. Image courtesy of Gregg Semenza (PMID: 21620138).](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0007.jpg)
![Western Blot: Glut1 Antibody [NB110-39113] - Glut1 in human brain lysate (55 kDa). Antibody at 1:500. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB110-39113-img0016.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Glut1 Antibody [NB110-39113] - Flow cytometry analysis using the PE conjugate of NB110-39113. Staining of Glut 1 expression on CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. FMO control (red) and isotype control (blue, NBP2-24983) were compared to CD4+ T cells (orange), and this PE conjugated Glut 1 antibody positively stained CD4+ lymphocytes isolated from Mouse. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glut1-Antibody-Flow-Cytometry-NB110-39113-img0014.jpg)
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-NBP1-48309-img0025.jpg "Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunostaining of original tumor, low passage, and high passage KCI-MENG1 cells. The original patient-derived tumor (top row) showed moderate and patchy immunoreactivity for epithelial membrane antigen (EMA); strong and diffuse immunostaining for progesterone receptor (PR); and a Ki-67 proliferative index of 2-3%. There was also strong immunostaining for N-cadherin and vimentin. KCI-MENG1-LP cells (middle row) and KCI-MENG1-HP cells (bottom row) maintained expression of EMA, N-cadherin, and vimentin but had significantly reduced PR expression compared to the original tumor. Whereas Ki-67 labeling was found in only a small number of cells in the original tumor and low passage cells, it was positive in virtually all P84 cells. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.")
![Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N cadherin (NBP1-48309) and B-actin antibody (NB600-501). The corresponding secondary antibodies used were either goat anti-rabbit IgG-HRP (NB7160) or goat anti-mouse IgG-HRP (NB7539). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0029.jpg)
![Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of N-Cadherin expression in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0005.jpg)
![Immunocytochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Human meningioma mouse xenograft model KCI-MENG1-LPSX generated with the spontaneously immortal cell line KCI-MENG1-LP. Tumors from immunocompromised SCID mice were dissected and the derivative cell line KCI-MENG1-LPSX CL was generated. The EMA, PR, and N-cadherin IHC of the mouse tumor highly resembled the original patient-derived tumor. The vimentin- and Ki-67-stained cells in the mouse tumor tissue were markedly more abundant and more intensely stained than in the original tumor. KCI-MENG1-LPSX CL cells displayed the same patterns of immunostaining as the high passage parent cell line KCI-MENG1-HP, including the loss of PR staining. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.translational-medicine.com/content/13/1/227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-NBP1-48309-img0026.jpg)
![Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Intracellular-NBP1-48309-img0010.jpg)
![Simple Western: N-Cadherin Antibody (13A9) [NBP1-48309] - Simple Western lane view shows a specific band for N Cadherin in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Simple-Western-NBP1-48309-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: N-Cadherin Antibody (13A9) [NBP1-48309] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Western-Blot-NBP1-48309-img0032.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - Adult mouse neural stem cells stained for N-Cadherin. Antibody at 1:100. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-Immunofluorescence-NBP1-48309-img0027.jpg)
![Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - Immunohistochemical characterization of tissue cultures. Nuclei labeled with DAPI (blue). Whole-mount immunostaining of a cardiomyocyte-only spheroid 1 month in culture, N-cadherin (red) and EH-myomesin (green). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32118040/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-NBP1-48309-img0031.jpg)
![Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on U-251 MG cells with N-Cadherin Antibody (13A9) NBP1-48309AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Cytometry-NBP1-48309-img0030.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunocytochemistry-Immunofluorescence-NBP1-48309-img0021.jpg)
![Immunohistochemistry: N-Cadherin Antibody (13A9) [NBP1-48309] - IHC analysis of N Cadherin in mouse liver using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-NBP1-48309-img0007.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (13A9) [NBP1-48309] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of N-Cadherin antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Immunohistochemistry-Paraffin-NBP1-48309-img0028.jpg)
![Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-13A9-Flow-Cytometry-NBP1-48309-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0005.jpg "Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Aquaporin-2 Antibody NB110-74682 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0006.jpg)
![Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Western-Blot-NB110-74682-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NB110-74682] - Analysis of FFPE human kidney using 1:100 conc. of Aquaporin-2 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Lumenal staining of Aquaporin-2 in scattered tubules was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunohistochemistry-Paraffin-NB110-74682-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Aquaporin-2 Antibody [NB110-74682] - Staining of mpkCCD cell line](http://images.novusbio.com/fullsize/Aquaporin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74682-img0003.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Glut-1 was detected using NB300-666 in whole cell extracts.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0007.jpg "Western Blot: Glut1 Antibody [NB300-666] - Western blot analysis of 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and HIF1B (NB100-124) analysis, nuclear extracts were generated and analyzed. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY license. Glut-1 was detected using NB300-666 in whole cell extracts.")
![Western Blot: Glut1 Antibody [NB300-666] - WB analysis of purified Glucose Transporter (GLUT1) with GLUT1 antibody at a dilution of 1:2000.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0001.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Upregulation of Glut1 (NB300-666) and Glut3 (NBP2-66872) expression by Aqp9 gene deletion and ONC. Western blots for Glut1 and Glut3 protein expression levels in WT and Aqp9 KO mice with and without ONC. beta actin was used as a control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32748371/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0008.jpg)
![Western Blot: Glut1 Antibody [NB300-666] - Rat brain cortical extracts (10 ug total protein per lane) on 10% SDS-PAGE gel. Antibody diluted 1:2000.](http://images.novusbio.com/fullsize/Glut1-Antibody-Western-Blot-NB300-666-img0006.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - Human kidney distal tubules stained with E-CAD antibody. Secondary antibody: Donkey anti-Mouse Alexa Fluor 555. Imaging with a 40x objective from Zeiss Observer microscope with Apotome2. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP2-19051-img0011.jpg "Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - Human kidney distal tubules stained with E-CAD antibody. Secondary antibody: Donkey anti-Mouse Alexa Fluor 555. Imaging with a 40x objective from Zeiss Observer microscope with Apotome2. Image from verified customer review.")
![Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Western blots showing a reduction in epithelial mesenchymal transition (EMT) markers upon PDK1 knockdown in Colo205 and HT29 cells using E cadherin (NBP2-19051), N cadherin (NBP1-48309) and B-actin antibody (NB600-501). The corresponding secondary antibodies used were either goat anti-rabbit IgG-HRP (NB7160) or goat anti-mouse IgG-HRP (NB7539). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0013.jpg)
![Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of E-Cadherin in LNCAP (1), A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - ICC staining of E-Cadherin in human colon cancer sphere. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunocytochemistry-Immunofluorescence-NBP2-19051-img0010.jpg)
![Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Mitochondrial fission regulates migration and invasion in BRAF^V600E CRC cells through glucose metabolic reprograming. Western blot showing a reduction in EMT markers upon vemurafenib treatment in BRAF^V600E cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33738242/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (7H12) [NBP2-19051] - Human colonic tissue was stained with E-Cadherin antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunocytochemistry-Immunofluorescence-NBP2-19051-img0009.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin antibody in mouse colon cancer tissue. Antibody dilution 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP2-19051-img0012.jpg)
![Flow Cytometry: E-Cadherin Antibody (7H12) [NBP2-19051] - Analysis of Hela cells using E-Cadherin mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Flow-Cytometry-NBP2-19051-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: E-Cadherin Antibody (7H12) [NBP2-19051] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma, 6nM TNF alpha, and 3nM IL1 beta). Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Western-Blot-NBP2-19051-img0015.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (7H12) [NBP2-19051] - IHC staining of E-Cadherin in gastric cancer tissues (left) and lung cancer tissues (right) with DAB staining.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-7H12-Immunohistochemistry-Paraffin-NBP2-19051-img0003.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Analysis in human colon and liver tissues using NBP1-82574 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Analysis in human colon and liver tissues using NBP1-82574 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human colon, kidney, liver and small intestine using Anti-SLC9A3 antibody NBP1-82574 (A) shows similar protein distribution across tissues to independent antibody NBP1-82575 (B).](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0025.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human liver shows no membranous positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0028.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human colon shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0024.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human gallbladder shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0026.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human kidney shows weak positivity in apical membrane in cells in tubules.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0027.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0029.jpg)
![Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Western-Blot-NB110-81529-img0005.jpg "Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.")
![Immunohistochemistry: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Immunofluorescence-NB110-81529-img0008.jpg)
![Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Simple-Western-NB110-81529-img0006.jpg)
![Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.](http://images.novusbio.com/fullsize/MRP1-Antibody-IU2H10-Dual-RNAscope-ISH-IHC-NB400-156-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/MRP1-Antibody-IU2H10-Immunocytochemistry-Immunofluorescence-NB400-156-img0007.jpg)
![Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)](http://images.novusbio.com/fullsize/MRP1-Antibody-IU2H10-Western-Blot-NB400-156-img0006.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0005.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using NB300-147.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Immunocytochemistry-Immunofluorescence-NB300-147-img0008.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) [NB300-147] - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using NB 300-147. Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-464-8-also-known-as-8A-Western-Blot-NB300-147-img0007.jpg)