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186 Results for "novus"

NB120-19294

LAMP-1/CD107a Antibody

(2 Reviews)

(33 Publications)

100 μg

NB120-19294

Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Analysis of 20 ug of HeLa cell lysate and 5 ul of Molecular Weight Protein Ladder per well.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin D (CathD) expression and localization in primary neurons. Colocalization of transfected CathD with LAMP-1/CD107a. CathD transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathD. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Lamp1 (red) in HeLa cells.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin B (CathB) expression and localization in primary neurons. Colocalization of transfected CathB with LAMP-1/CD107a. CathB transfected neurons were examined by co-immunostaining of LAMP-1/CD107a and CathB. Yellow colored cytoplasmic spots are indicative of co-localization of transfected cathepsins and LAMP-1/CD107a. Scale bar = 10 micron. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-6-37), licensed under a CC-BY license.

EsxG-EsxH alters phagosomal GAL3, ubiquitin, and LAMP1. (A, C, and E) IF images of GAL3 (A), ubiquitin (FK2 antibody) (C), and LAMP1 (E) in BMDMs that were infected with DsRed-expressing H37Rv (WT) or the deltaesxH mutant for 3 h. Images are maximum-intensity projections. Scale bar, 10 um. Boxed areas in the merged image are shown in higher magnification in the rightmost panel. Mtb, M. tuberculosis. (B, D, and F) Automated image analysis was used to quantify the MFI of GAL3 (B), ubiquitin (D), and LAMP1 (F) colocalized with individual bacilli from 5 fields of a 12-mm coverslip. Data are means +/- SEM from one representative experiment from three (A, B, E, and F) or two (C and D) independent experiments. ****, P <= 0.0001, Student's t test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30482832), licensed under a CC-BY licence.

Inhibition of H4K12ac with NU9056 modulates cytokine and chemokine production. After 5–7 days of differentiation, MDDCs were treated with NU9056 50 nM, 0.2% EtOH or both for 5 days. Supernatants collected were analyzed for 48 inflammatory cytokines and chemokines. Data is from 3 blots for untreated control, 2 blots each for treatment with 0.2% EtOH alone, treatment with NU9056 alone, and treatment with 0.2% EtOH plus NU9056. Cytokines chosen to be presented in the graph were selected on the basis of fold change (2 folds or more) compared to control. Each cytokine is detected in duplicates within each blot. Statistical differences were calculated using student’s t-test when individually compared to untreated control and indicated with a * (compared to control) and # (between treatments) for significant p value. Two-way ANOVA was carried out (p = 0.027). Panel a shows optical density values for cytokines IL-15, RANTES, TGF-beta 1 and TNF-alpha . Panel b shows optical density values for MCP2. In panel c, MCP-2 ELISA was carried out from cell culture medium at least three times in duplicates. Values detected in pg/mL are as follows, untreated control (322.4 ± 25.04), NU9056 50 nM (317.5 ± 14.3), 0.2% EtOH (401.7 ± 28.2) and 0.2% EtOH + NU9056 50 nM (321.6 ± 13.5, p = 0.03). Panels d–g show intracellular staining to measure MCP-2 levels in untreated or treated MDDCs. Data represented is from at least 3 experiments. Panel d shows % gated MCP-2 positive cells for untreated control (52.3% ± 2.3), NU9056 50 nM (45.8% ± 1.07), 0.2% EtOH (85.8% ± 5.2, p = 0.002) and 0.2% EtOH + NU9056 50 nM (29.6% ± 1.18, p = 0.0001). Panel e shows MFI values for MCP-2-FITC for untreated control (2510 ± 29), NU9056 50 nM (2151 ± 43), 0.2% EtOH (4260 ± 603, p = 0.04) and 0.2% EtOH + NU9056 50 nM (1459 ± 297, p = 0.01). Panel f shows an overlay histogram of MCP-2-FITC for all the samples as shown in legend. Panel g shows representative single cell images. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41598-017-11172-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

DDR and NF-kappa B are activated concomitantly in senescent MEFs and aged tissues. (A) Immunoblot detection of p-p65 and total p65 in liver tissue from 16-week-old WT (n=3) and Ercc1-/ delta (n=3) mice. (B) Immunoblot detection of phosphorylation of ATM and downstream targets gamma H2AX and p21 in liver from 16-week-old WT and Ercc1-/ delta mice. (C) Immunoblot detection of phosphorylation of NF-kappa B and I kappa B alpha in liver lysates from 3, 12 and 24 month-old WT mice. n=3 mice per group. (D) Immunoblot detection of p-ATM, ATM and p21 in the same liver lysates. (E) Immunoblot detection of DDR effectors in nuclear extracts from passage 5 WT and Ercc1-/- MEFs, grown at 20% oxygen. (F) Level of NF-kappa B activation is higher in Ercc1-/- MEFs compared to WT MEFs at passage 5, as measured by Immunoblot detection of p-p65 and total p65 in WT and Ercc1-/- MEFs at passage 5 after culturing in 20% oxygen. (G) Representative images of immunofluorescent detection of p65 and NEMO in passage 4 WT and Ercc1-/- MEFs grown at 20% oxygen. Blue: DAPI staining; Green: p65 (top panel) or NEMO (bottom panel). Images were taken at the magnification of 60x. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32201398), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-19294AF350
Alexa Fluor 405	NB120-19294AF405
Alexa Fluor 488	NB120-19294AF488
Alexa Fluor 532	NB120-19294AF532
Alexa Fluor 594	NB120-19294AF594
Alexa Fluor 647	NB120-19294AF647
Alexa Fluor 700	NB120-19294AF700
Alexa Fluor 750	NB120-19294AF750
Allophycocyanin	NB120-19294APC
Biotin	NB120-19294B
DyLight 350	NB120-19294UV
DyLight 405	NB120-19294V
DyLight 488	NB120-19294G
DyLight 550	NB120-19294R
DyLight 594	NB120-19294DL594
DyLight 650	NB120-19294C
DyLight 680	NB120-19294FR
DyLight 755	NB120-19294IR
FITC	NB120-19294F
HRP	NB120-19294H
Janelia Fluor 549	NB120-19294JF549
Janelia Fluor 646	NB120-19294JF646
PE	NB120-19294PE
PerCP	NB120-19294PCP
mFluor Violet 450 SE	NB120-19294MFV450
mFluor Violet 500 SE	NB120-19294MFV500
mFluor Violet 610 SE	NB120-19294MFV610

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NB600-1008

LYVE-1 Antibody

(3 Reviews)

(30 Publications)

0.2 mg

NB600-1008

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Positive staining for Lyve-1 on FFPE Mouse Liver, 20x magnification. Antibody diluted 1:800. Image from verified customer review.

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse kidney stained with LYVE-1 antibody. Image from verified customer review.

Western Blot: LYVE-1 Antibody [NB600-1008] - Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Zeb1-regulation of alkali-induced corneal NV in mice. c1 A representative stereoscopic image of the Zeb1-/+ and d1 Zeb1+/+ corneas and c2-d2 their whole flat-mount corneas immunostained with the endothelium marker CD31 and the lymphatic vessel marker LYVE-1. *p = 0.05; **p = 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32620870/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse liver. Antibody at 1:800. Detection by Polymer-HRP. Image from verified customer review.

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Hypoxia, blood vessel and proliferation staining. IHC staining in the Biopsy 23 and Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 and LYVE1. 15x Magnification. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/4/3/821), licensed under a CC-BY license.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-52721

LAMP-1/CD107a Antibody (5E7) - BSA Free

(2 Reviews)

(7 Publications)

0.1 mg

NBP2-52721

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - ICC usage of NBP2-52721. Image submittied via verified customer review.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all the ductal and tubular epithelial cells.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-LAMP (5E7) at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - MCF-7 human breast cancer cell line. Secondary antibody goat anti-mouse IgG Fc Dy680 (NBP1-72880). Image from verified customer review.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (5E7) [NBP2-52721] - Analysis of FFPE human kidney tissue section using LAMP-1 antibody (clone 5E7) at 1:100 dilution. The antibody generated a specific cytoplasmic staining (with dotted appearance) in all cells, especially the ductal and tubular epithelial cells. Few cells from the glomeruli showed nuclear signal also.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-52721AF350
Alexa Fluor 405	NBP2-52721AF405
Alexa Fluor 488	NBP2-52721AF488
Alexa Fluor 532	NBP2-52721AF532
Alexa Fluor 594	NBP2-52721AF594
Alexa Fluor 647	NBP2-52721AF647
Alexa Fluor 700	NBP2-52721AF700
Alexa Fluor 750	NBP2-52721AF750
Allophycocyanin	NBP2-52721APC
Biotin	NBP2-52721B
DyLight 350	NBP2-52721UV
DyLight 405	NBP2-52721V
DyLight 488	NBP2-52721G
DyLight 550	NBP2-52721R
DyLight 594	NBP2-52721DL594
DyLight 650	NBP2-52721C
DyLight 680	NBP2-52721FR
DyLight 755	NBP2-52721IR
FITC	NBP2-52721F
HRP	NBP2-52721H
Janelia Fluor 549	NBP2-52721JF549
Janelia Fluor 646	NBP2-52721JF646
PE	NBP2-52721PE
PerCP	NBP2-52721PCP
mFluor Violet 450 SE	NBP2-52721MFV450
mFluor Violet 500 SE	NBP2-52721MFV500
mFluor Violet 610 SE	NBP2-52721MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52721SS
Azide and BSA Free	NBP2-80824

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NB100-725

LYVE-1 Antibody - BSA Free

(2 Reviews)

(12 Publications)

0.1 ml

NB100-725

Western Blot: LYVE-1 Antibody [NB100-725] - Western Blot: [NB100-725] - Total protein from human stomach, lymph node and placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LYVE1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB100-725] - Analysis using the DyLight 488 conjugate of NB100-725. Staining of LYVE1 in paraffin embedded MDA-MB-231 breast cancer orthotopic transplantation tissue. Image courtesy of product review submitted by Luana Schito.

Immunohistochemistry: LYVE-1 Antibody [NB100-725] - Detection of LYVE1 in endothelial cells of human lung blood vessels using NB100-725. Note presence of RBCs within vessel lumen.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - Analysis using the DyLight 488 conjugate of NB100-725. Unlabeled control with labeled samples of cells dissociated mechanically and enzymatically from human skin. Image provided via product review by Patricia Redondo.

Western Blot: LYVE-1 Antibody [NB100-725] - Analysis of extracts from A549 cells using LYVE1 antibody (NB100-725, 1:100). Image from verified customer review.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - LYVE1 antibody was tested at 1:400 in A549 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).

Flow Cytometry: LYVE-1 Antibody [NB100-725] - An intracellular stain was performed on A549 cells with LYVE-1 Antibody NB100-725 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunohistochemistry: LYVE-1 Antibody [NB100-725] - Detection of LYVE1 in endothelial cells of human bladder vasculature using NB100-725.

Immunohistochemistry: LYVE-1 Antibody [NB100-725] - Detection of LYVE1 in endothelial cells of human lung blood vessels using NB100-725.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-725. Staining of LYVE1 in human dissociated skin lyve1+ using AF488 conjugated anti-LYVE1 antibody. Image from verified customer review.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - An intracellular stain was performed on A549 cells with LYVE-1 Antibody NB100-725G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 488.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - An intracellular stain was performed on K562 cells with LYVE-1 Antibody NB100-725C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-725AF350
Alexa Fluor 405	NB100-725AF405
Alexa Fluor 488	NB100-725AF488
Alexa Fluor 532	NB100-725AF532
Alexa Fluor 594	NB100-725AF594
Alexa Fluor 647	NB100-725AF647
Alexa Fluor 700	NB100-725AF700
Alexa Fluor 750	NB100-725AF750
Allophycocyanin	NB100-725APC
Allophycocyanin/Cy7	NB100-725APCCY7
Biotin	NB100-725B
DyLight 350	NB100-725UV
DyLight 405	NB100-725V
DyLight 488	NB100-725G
DyLight 550	NB100-725R
DyLight 594	NB100-725DL594
DyLight 650	NB100-725C
DyLight 680	NB100-725FR
DyLight 755	NB100-725IR
FITC	NB100-725F
HRP	NB100-725H
Janelia Fluor 549	NB100-725JF549
Janelia Fluor 646	NB100-725JF646
PE	NB100-725PE
PE/Atto594	NB100-725PEATT594
PE/Cy5.5	NB100-725PECY55
PE/Cy7	NB100-725PECY7
PerCP	NB100-725PCP
mFluor Violet 450 SE	NB100-725MFV450
mFluor Violet 500 SE	NB100-725MFV500
mFluor Violet 610 SE	NB100-725MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-725SS

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NB120-13253

Rab5a Antibody

(2 Reviews)

(16 Publications)

0.1 mg

NB120-13253

Western Blot: Rab5a Antibody [NB120-13253] - Western blot analysis of Human Cell line lysates showing detection of Rab5aprotein using Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x.

Immunohistochemistry: Rab5a Antibody [NB120-13253] - Immunohistochemistry analysis using Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NB120-13253] - IF staining of Rab5a in hepatocytes. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 20x.

Immunohistochemistry-Paraffin: Rab5a Antibody [NB120-13253] - analysis using Rabbit Anti-Rab5 Polyclonal Antibody . Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

Reactivity:

Hu, Mu, Rt, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-36568

EEA1 Antibody (6D4.1D4) - BSA Free

(3 Publications)

0.1 mg

NBP2-36568

Western Blot: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of partial recombinant human EEA1 protein (14kDa) with EEA1 antibody clone 6D4.1D4 at 0.5 ug mL.

Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of human testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein.

Flow (Intracellular): EEA1 Antibody (6D4.1D4) [NBP2-36568] - Intracellular staining of human EEA1 in Flow cytometry using 2.5 ug of antibody per 1 million cells. Isotype control was mouse IgG1.

Immunohistochemistry-Paraffin: EEA1 Antibody (6D4.1D4) [NBP2-36568] - Analysis of FFPE tissue section of mouse testes using mouse monoclonal EEA1 antibody (clone 6D4.1D4) at 10 ug/ml concentration. The interstitial cells depicted a punctate to granular cytoplasmic staining pattern for EEA1 protein with no signal in other cell types.

Reactivity:

Hu, Mu, Rt, Po, Bt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-36568AF350
Alexa Fluor 405	NBP2-36568AF405
Alexa Fluor 488	NBP2-36568AF488
Alexa Fluor 532	NBP2-36568AF532
Alexa Fluor 594	NBP2-36568AF594
Alexa Fluor 647	NBP2-36568AF647
Alexa Fluor 700	NBP2-36568AF700
Alexa Fluor 750	NBP2-36568AF750
Allophycocyanin	NBP2-36568APC
Biotin	NBP2-36568B
DyLight 350	NBP2-36568UV
DyLight 405	NBP2-36568V
DyLight 488	NBP2-36568G
DyLight 550	NBP2-36568R
DyLight 594	NBP2-36568DL594
DyLight 650	NBP2-36568C
DyLight 680	NBP2-36568FR
DyLight 755	NBP2-36568IR
FITC	NBP2-36568F
HRP	NBP2-36568H
Janelia Fluor 549	NBP2-36568JF549
Janelia Fluor 646	NBP2-36568JF646
PE	NBP2-36568PE
PerCP	NBP2-36568PCP
mFluor Violet 450 SE	NBP2-36568MFV450
mFluor Violet 500 SE	NBP2-36568MFV500
mFluor Violet 610 SE	NBP2-36568MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-36568SS
Azide and BSA Free	NBP2-80711

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NB100-292

TERF2IP Antibody

(21 Publications)

100 ul

NB100-292

Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292] - Sections of human lung carcinoma.~~ Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

<b>Independent Antibodies Validation. </b>Immunoprecipitation: TERF2IP Antibody [NB100-292] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-RAP1 antibody used for IP at 6 ug per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-RAP1 antibody. For blotting immunoprecipitated RAP1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-292AF350
Alexa Fluor 405	NB100-292AF405
Alexa Fluor 488	NB100-292AF488
Alexa Fluor 532	NB100-292AF532
Alexa Fluor 594	NB100-292AF594
Alexa Fluor 647	NB100-292AF647
Alexa Fluor 700	NB100-292AF700
Alexa Fluor 750	NB100-292AF750
Allophycocyanin	NB100-292APC
Biotin	NB100-292B
DyLight 350	NB100-292UV
DyLight 405	NB100-292V
DyLight 488	NB100-292G
DyLight 550	NB100-292R
DyLight 594	NB100-292DL594
DyLight 650	NB100-292C
DyLight 680	NB100-292FR
DyLight 755	NB100-292IR
FITC	NB100-292F
HRP	NB100-292H
Janelia Fluor 549	NB100-292JF549
Janelia Fluor 646	NB100-292JF646
PE	NB100-292PE
PerCP	NB100-292PCP

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NBP1-30914

EEA1 Antibody

(1 Review)

(6 Publications)

0.1 ml

NBP1-30914

Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug each lane of HEK-293 cell extracts. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 protein stained by EEA1 antibody at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody (NBP2-43561) at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Paraffin-embedded colon carcinoma. Antibody at 1:500.

Western Blot: EEA1 Antibody [NBP1-30914] - Mouse brain. 5% SDS PAGE. Antibody at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat 2 whole cell lysate/extract. 5% SDS-PAGE. Antibody at 1:1000.

Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug whole cell lysate/extracts B. Antibody at 1:5000.

Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - Methanol-fixed HeLa cells, using EEA1 (NBP1-30914) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled red.

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term (GTX109638) dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Reactivity:

Hu, Mu, Rt, Ha

Species Glossary

Reactivity: Hu, Mu, Rt, Ha

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-25183

LAMP-1/CD107a Antibody (H4A3) - Azide and BSA Free

(1 Publication)

0.1 mg

NBP2-25183

Western Blot: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human peripheral blood cells.

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - HeLa cells.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - IHC staining of LAMP-1 [H4A3] catalog number NBP2-25183 in mouse liver. Cytoplasmic and membrane staining was observed in tubule cells and glomeruli.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.

Immunohistochemistry-Paraffin: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Human kidney.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A549 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on THP-1 cells with LAMP-1/CD107a antibody (H4A3) NBP2-25183PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) azide free, GAM-APC.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) APC.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Staining of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor (R) 488.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - Analysis of PHA-activated PBMC with anti-CD107a (H4A3) PerCP-CyTM5.5.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on A431 cells with LAMP-1/CD107a Antibody [H4A3] NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: LAMP-1/CD107a Antibody (H4A3) - Azide Free [NBP2-25183] - An intracellular stain was performed on U-87 cells with LAMP-1/CD107a Antibody (H4A3) NBP2-25183AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25183AF350
Alexa Fluor 405	NBP2-25183AF405
Alexa Fluor 488	NBP2-25183AF488
Alexa Fluor 532	NBP2-25183AF532
Alexa Fluor 594	NBP2-25183AF594
Alexa Fluor 647	NBP2-25183AF647
Alexa Fluor 700	NBP2-25183AF700
Alexa Fluor 750	NBP2-25183AF750
Allophycocyanin	NBP2-25183APC
Allophycocyanin/Cy7	NBP2-25183APCCY7
Biotin	NBP2-25183B
DyLight 350	NBP2-25183UV
DyLight 405	NBP2-25183V
DyLight 488	NBP2-25183G
DyLight 550	NBP2-25183R
DyLight 594	NBP2-25183DL594
DyLight 650	NBP2-25183C
DyLight 680	NBP2-25183FR
DyLight 755	NBP2-25183IR
FITC	NBP2-25183F
HRP	NBP2-25183H
Janelia Fluor 549	NBP2-25183JF549
Janelia Fluor 646	NBP2-25183JF646
PE	NBP2-25183PE
PE/Atto594	NBP2-25183PEATT594
PE/Cy5.5	NBP2-25183PECY55
PerCP	NBP2-25183PCP
mFluor Violet 450 SE	NBP2-25183MFV450
mFluor Violet 500 SE	NBP2-25183MFV500
mFluor Violet 610 SE	NBP2-25183MFV610

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NBP1-87497

Syntaxin 7 Antibody

(1 Review)

(2 Publications)

0.1 ml

NBP1-87497

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Analysis in human lymph node and pancreas tissues. Corresponding Syntaxin 7 RNA-seq data are presented for the same tissues.

<b>Orthogonal Strategies Validation. </b>Western Blot: Syntaxin 7 Antibody [NBP1-87497] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Immunocytochemistry/Immunofluorescence: Syntaxin 7 Antibody [NBP1-87497] - Staining of human cell line U-251 MG shows localization to lysosomes.

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.

Western Blot: Syntaxin 7 Antibody [NBP1-87497] - Analysis in human cell line Daudi.

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human kidney shows strong membranous positivity in cells in tubules.

Immunohistochemistry-Paraffin: Syntaxin 7 Antibody [NBP1-87497] - Staining of human lymph node shows strong membranous positivity in germinal center cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-87174

Rab7a Antibody

(1 Review)

(3 Publications)

0.1 ml

NBP1-87174

Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RAB7A antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.

Western Blot: Rab7a Antibody [NBP1-87174] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00338382-M01

RAB7B Antibody (3B3)

(2 Reviews)

(7 Publications)

0.1 mg

H00338382-M01

Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - RAB7B monoclonal antibody (M01), clone 3B3. Analysis of RAB7B expression in human kidney.

Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - RAB7B expression in HeLa ( Cat # L013V1 ).

Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - RAB7B monoclonal antibody (M01), clone 3B3. Analysis of RAB7B expression in A-431.

Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - Analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody (M01), clone 3B3.Lane 1: RAB7B transfected lysate(22.5 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: RAB7B Antibody (3B3) [H00338382-M01] - Analysis of RAB7B transfected lysate using anti-RAB7B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB7B MaxPab rabbit polyclonal antibody.

ELISA: RAB7B Antibody (3B3) [H00338382-M01] - Detection limit for recombinant GST tagged RAB7B is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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