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44 Results for "novus"

H00023435-M01

TDP-43/TARDBP Antibody (2E2-D3)

(3 Reviews)

(210 Publications)

0.1 mg

H00023435-M01

Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP monoclonal antibody (M01), clone 2E2-D3 Analysis of TARDBP expression in A-431.

Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP in rat tissue samples. Image courtsey of product review by Daniel Pirici.

Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - PDI co-precipitates with TDP-43/TARDBP and is increased in mutant TDP-43 transgenic mouse spinal cords. Co-immunoprecipitation of wildtype and mutant Q331K TDP-43-mCherry from Neuro2a cell lysates using an anti-PDI antibody followed by TDP-43 immunoblot. Control immunoprecipitations were performed using untransfected (Untrans) or mCherry expressing cell lysates, buffer only with coprecipitating antibodies or TDP-43 Q331K cell lysates using an irrelevant, isotype-matched control antibody (IgG). Input control (2%) shows expression of mCherry-TDP43 in wildtype and Q331K cell lysates. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081170) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]

Western Blot: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody (M01), clone 2E2-D3.Lane 1: TARDBP transfected lysate(44.7 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of monoclonal antibody to TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma tissue Antibody concentration 3 ug/ml.

<b>Genetic Strategies Validation. </b>Knockdown Validated: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi ( Cat # H00023435-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TARDBP monoclonal antibody (M01), clone 2E2-D3 (Cat # H00023435-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

ELISA: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - Detection limit for recombinant GST tagged TARDBP is 0.3 ng/ml as a capture antibody.

SINE compounds partially rescue motor deficits in an animal model of ALS/FTD. (b) Western blot demonstrating the expression of human TDP43 within the brains of AAV9-injected animals, which we have previously shown is largely restricted to neuronal cell types (Jackson et al.59). Full-length blots appear in Fig. S1. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-22858-w), licensed under a CC-BY licence.

CLIP shows that TDP-43 directly interacts with translational target mRNAs. (C and D) Plots showing percentage of input mRNA in the TDP-43 IP for the mRNAs indicated in three separate CLIP assays from MN1 cells. Performing CLIP from UV-treated cells leads to significant recovery and enrichment relative to Gapdh for all mRNAs examined (C). In the absence of UV treatment, much less mRNA is recovered and no significant enrichment relative to Gapdh mRNA is observed. Note that the Y-axis scale in D is 1000x smaller than in C, indicating strong crosslinking dependence of mRNA co-immunoprecipitation (n = 3, s.e.m. error bars, P-values indicated in the plot, unpaired two-tailed t-test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30357366), licensed under a CC-BY licence.

PDI co-precipitates with TDP-43 and is increased in mutant TDP-43 transgenic mouse spinal cords.(A) Co-immunoprecipitation of wildtype and mutant Q331K TDP-43-mCherry from Neuro2a cell lysates using an anti-PDI antibody followed by TDP-43 immunoblot. Control immunoprecipitations were performed using untransfected (Untrans) or mCherry expressing cell lysates, buffer only with coprecipitating antibodies or TDP-43 Q331K cell lysates using an irrelevant, isotype-matched control antibody (IgG). Input control (2%) shows expression of mCherry-TDP43 in wildtype and Q331K cell lysates. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0081170), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, Ba, I, Pm, Pp

Species Glossary

Reactivity: Hu, Mu, Rt, Ba, I, Pm, Pp

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB600-241

Nucleolin Antibody

(2 Reviews)

(25 Publications)

0.1 ml

NB600-241

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Analysis of Nucleolin MCF-7 breast cancer cells. Image courtsey of product review by Lacey Litchfield.

Simple Western: Nucleolin Antibody [NB600-241] - Image shows a specific band for Nucleolin in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Endogenous nuclear nucleolin-COUP-TFII interaction in MCF-7 and T47D cells.NE (200A ug protein) from MCF-7 cells. Immunofluorescent staining of endogenous COUP-TFII (green) and nucleolin (red) in the nuclei (Hoechst, blue) of MCF-7 cells. Merged images are shown at the right. Bar is 10A um. Image collected and cropped by Citeab from the following publication (Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor transcription in breast cancer cells. PLoS One (2012) licensed under a CC-BY license.

Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). Image from verified customer review.

Immunohistochemistry: Nucleolin Antibody [NB600-241] - Detection of Nucleolin in human tonsil germinal center.

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB600-241] - In-situ immunofluorescent staining of PD31 murine pre-B cells.

HT-LKO cells recapitulate the hallmarks of lamin A/C deficiency. (c) Western blot for lamin A/C reveals the absence of both proteins in HT-LKO cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27461848), licensed under a CC-BY licence.

FASN and CAD Binding Specificity of pVHL Mutants Derived from VHL Patients. (A) Schematic representation of pVHL domains. Dotted lines indicate the approximate positions of the HIF-1 alpha and Elongin C binding domains. The alpha and beta structural domains are also indicated. (B) Effects of pVHL mutations on interactions with E3 ligase components, HIF-alpha and other interacting proteins including FASN. pVHL mutants were stably expressed by lentiviral vectors in HCT116 cells, and WCEs were tested in precipitation assays. Note that the mutant pVHL-W88S almost completely lost the ability to bind to HIF-1 alpha and HIF-2 alpha , whereas pVHL-Y112H retains this ability almost completely. However, both pVHL-W88S and pVHL-Y112H show significantly reduced binding to FASN and CAD, as indicated by small and large asterisks, respectively. Arrows indicate HIF-1 alpha and HIF-2 alpha bands. Long arrows and arrowheads indicate unmodified and modified forms of FASN, respectively. Protein samples prepared from hypoxia (1% oxygen)-exposed cells were added as positive control in left-end extra lanes. (C) The same samples as used in B were analyzed by silver staining. The E3 ligase components appear as major bands as indicated. (D) Subcellular localization of stably expressed pVHL mutants in 786-O. pVHL-Y112H and pVHL-W88S showed preferential nuclear localization, whereas pVHL-WT and pVHL-H115Q which both strongly bind to FASN and CAD, showed cytoplasmic localization. Cells showing clear nuclear (C N) localization in three microscopic viewing areas were counted as shown in the lower panel. The total counted cell numbers ranged from 35 to 64. The experiments were repeated more than twice, except for the cell localization count, and the results were reproduced. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28775317), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pl

Species Glossary

Reactivity: Hu, Mu, Rt, Pl

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB100-1762

LSD1 Antibody (1B2E5)

(12 Publications)

0.1 ml

NB100-1762

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates.

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.

Simple Western: LSD1 Antibody (1B2E5) [NB100-1762] - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

<b>Genetic Strategies Validation. </b>Knockdown Validated: LSD1 Antibody (1B2E5) [NB100-1762] - Knockdown of KDM1A protein was confirmed by western blotting of whole-cell lysates from DAOY and ONS-76 cells. beta-actin served as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252778/) licensed under a CC-BY license.

Plasma membrane associated APE1/Ref-1 is bound to ABCA1 in response to acetylation. Cells transiently expressing wild type APE1/Ref-1-FLAG or mutant APE1/Ref-1(K6/7R)-FLAG were treated with 1 µM TSA for 1 h. (A) Whole cell lysates were immunoprecipitated using the monoclonal anti-ABCA1 antibody, followed by immunoblot with the anti-FLAG antibody. (B) For reverse immunoprecipitation, cell lysates were immunoprecipitated with anti-APE1/Ref-1 antibody followed by immunoblot analysis with the polyclonal anti-ABCA1 antibody. Blots were stripped and re-probed with anti-ABCA1 or FLAG antibodies to ensure equal protein loading and no contamination of cellular proteins. Similar results were observed in replicate experiments. Columns, mean (n = 2-3); bars, SE. *, p < 0.05 indicates a significantly different result from control cells according to unpaired t-tests. (C) The binding between APE1/Ref-1 and ABCA1 in the plasma membrane was visualized using with a Duolink II PLA system with primary polyclonal anti-APE1/Ref-1 and monoclonal anti-ABCA1 antibodies (PLA†). The PLA-specific fluorescence which represents the APE1/Ref-1-ABCA1 signal, and the DAPI nuclear staining are in red and blue, respectively. The experiment was repeated multiple times with similar results; the data shown here are from a representative experiment. Optical slices were examined using a 40× oil immersion objective with a 2× zoom factor. Scale bar, 20 µm (×80). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31261750), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Involvement of N- terminus and C- terminus HIF-2 alpha in IH-induced HIF-2 alpha degradation by calpains. A. Schematic diagram showing the full length, N-terminus ( delta bHLH and delta bHLH+ delta PAS) and C-terminus ( delta UR, delta UR + delta CTAD) deleted constructs. B. Western blot showing HIF-2 alpha protein in PC12 cells transiently transfected with the HIF-2 alpha full length and the two N- and C-terminus truncated constructs and exposed to normoxia (N) or IH. The N-terminus and C-terminus deleted HIF-2 alpha proteins were detected with antibody raised against HIF-2 alpha N-terminus (Acris Antibodies; AP23352PU-N) and C-terminus (Novus Biologicals; NB100-122) respectively. C. PC12 cell lysates expressing the N- and C-terminus deleted protein were incubated for 15 min with purified calpain-1 (3 mg/ml) in presence of 1 mM CaCl2 or 1 mM CaCl2+2 mM EDTA and HIF-2 alpha protein was analyzed by western blot. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24124516), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

PHD3 depletion stabilizes hypoxic p27 expression by increasing p27 half-life. a Cell cycle arrest at G0 and subsequent release shows an increase of p27 expression in siPHD3 exposed cells. b Quantification for p27 expression under PHD3 depletion at indicated time points after cell cycle release in HeLa and 786-O cells. Asterisk indicates significant difference (p < 0,05; n = 3). c Cell cycle arrest at G0 and inhibition of protein synthesis with cycloheximide indicate increased p27 stability in PHD3 depleted HeLa cells. d Quantification of p27 expression using siPHD3 or control at indicated time points. Four independent experiments (± SEM) are shown (p < 0,05; n = 4). e Analysis of p27 stability in 786–0 cells by cycloheximide chase during reoxygenation after 24 h hypoxia demonstrates markedly increased half-life of p27 upon PHD3 depletion Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26223520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-84264

SIX1 Antibody

(17 Publications)

0.1 ml

NBP1-84264

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining in human skeletal muscle and liver tissues . Corresponding SIX1 RNA-seq data are presented for the same tissues.

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunocytochemistry/Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB120-6102

hnRNP A2B1 Antibody (DP3B3) - BSA Free

(9 Publications)

0.1 ml

NB120-6102

Knockdown Validated: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and hnRNP A1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1.0 ug/ml of Mouse Anti-Human hnRNP A1 Monoclonal Antibody (Catalog # NB120-6102) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for hnRNP A1 at approximately 35 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HAP1 human near-haploid cell line and hnRNP A2B1 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with hnRNP A2B1 Antibody (Catalog # NB120-6102) O/N at 4C, followed by HRP-conjugated Secondary Antibody and ECL detection. A specific band was detected for hnRNP A2B1 (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 1/1000. The Ponceau stained transfers of each blot are shown. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

<b>Genetic Strategies Validation. </b>HAP1 WT and hnRNP A2B1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with hnRNP A2B1 antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Whole cell protein from PC12 (lane 1), NIH-3T3 (lane 2), A431 (lane 3) and HeLa (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP A2B1 at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence.

Immunocytochemistry/Immunofluorescence: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - The hnRNP A2B1 antibody was tested in HeLa cells at a 5ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.

Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - hnRNP A2B1 expression in HeLa cells. Lane 1= 15 ug of HeLa nuclear extracts Lane 2= Co-IP of hnRNP A2B1. Image and data from confirmed customer review.

Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-hnRNP-A2/B1 Clone: DP3B3. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. MDBK 5. MDCK 6. CHO

Reactivity:

Hu, Mu, Rt, Bv, Ca, Ch, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Pm

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB120-6102AF350
Alexa Fluor 405	NB120-6102AF405
Alexa Fluor 488	NB120-6102AF488
Alexa Fluor 532	NB120-6102AF532
Alexa Fluor 594	NB120-6102AF594
Alexa Fluor 647	NB120-6102AF647
Alexa Fluor 700	NB120-6102AF700
Alexa Fluor 750	NB120-6102AF750
Biotin	NB120-6102B
DyLight 350	NB120-6102UV
DyLight 405	NB120-6102V
DyLight 488	NB120-6102G
DyLight 550	NB120-6102R
DyLight 594	NB120-6102DL594
DyLight 650	NB120-6102C
DyLight 680	NB120-6102FR
DyLight 755	NB120-6102IR
FITC	NB120-6102F
HRP	NB120-6102H
Janelia Fluor 549	NB120-6102JF549
Janelia Fluor 646	NB120-6102JF646
mFluor Violet 450 SE	NB120-6102MFV450
mFluor Violet 500 SE	NB120-6102MFV500
mFluor Violet 610 SE	NB120-6102MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB120-6102SS
Azide and BSA Free	NBP2-80777

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NBP1-89527

TOP2B Antibody

(2 Reviews)

(5 Publications)

0.1 ml

NBP1-89527

Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP1-89527] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human pancreas shows weak to moderate nuclear positivity in islets of Langerhans and exocrine glandular cells.

Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human kidney shows moderate nuclear positivity in cells in glomeruli and tubules.

Simple Western: TOP2B Antibody [NBP1-89527] - Simple Western lane view shows a specific band for TOP2B in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.

Simple Western: TOP2B Antibody [NBP1-89527] - Electropherogram image(s) of corresponding Simple Western lane view. TOP2B antibody was used at 1:100 dilution on RT-4 lysate(s).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-297

TERT Antibody (2D8)

(9 Publications)

0.1 ml

NB100-297

Western Blot: TERT Antibody (2D8) [NB100-297] - Analysis of human TERT, using NB100-297. Sample: MJ90 whole cell lysate.

Immunocytochemistry/Immunofluorescence: TERT Antibody (2D8) [NB100-297] - Detection of Tert (green) in HepG2 cells using NB100-297. Nuclei (Blue) are counterstained with Hoechst 33258.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-80735

SNRPD3 Antibody

(4 Publications)

0.1 ml

NBP1-80735

Western Blot: SNRPD3 Antibody [NBP1-80735] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SNRPD3 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SNRPD3 Antibody [NBP1-80735] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.

Immunohistochemistry-Paraffin: SNRPD3 Antibody [NBP1-80735] - Immunohistochemical staining of human small intestine shows moderate to strong nuclear positivity in glandular cells.

Western Blot: SNRPD3 Antibody [NBP1-80735] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: SNRPD3 Antibody [NBP1-80735] - Immunohistochemical staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: SNRPD3 Antibody [NBP1-80735] - Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SNRPD3 Antibody [NBP1-80735] - Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-87822

Thymopoietin/LAP2 Antibody

(2 Publications)

0.1 ml

NBP1-87822

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human skeletal muscle shows moderate positivity in nuclear membrane in a small subset of myocytes.

<b>Genetic Strategies Validation. </b>Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-TMPO antibody. Remaining relative intensity is presented.

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human lymph node shows strong positivity in nuclear membrane in germinal center cells.

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human prostate shows strong positivity in nuclear membrane in glandular cells.

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human skeletal muscle shows moderate positivity in nuclear membrane in a small subset of myocytes.

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human small intestine shows strong positivity in nuclear membrane in glandular cells.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining in human lymph node and skeletal muscle tissues using NBP1-87822 antibody. Corresponding TMPO RNA-seq data are presented for the same tissues.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-31413

RPL5 Antibody

(1 Publication)

0.1 ml

NBP1-31413

Western Blot: RPL5 Antibody [NBP1-31413] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPL5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: RPL5 Antibody [NBP1-31413] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL5 protein stained by RPL5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413] - Mouse hind brain. RPL5 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: RPL5 Antibody [NBP1-31413] - A. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE RPL5 antibody dilution: 1:1000

Western Blot: RPL5 Antibody [NBP1-31413] - A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413] - Gastric CA N87 xenograft, using RPL5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

<b>Genetic Strategies Validation. </b>Knockdown Validated: RPL5 Antibody [NBP1-31413] - Western Blot analysis of RPL5 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression level of RPL5 in the treated shRNA Rpl5 compared to the control mice. Image collected and cropped by CiteAb from the following publication (www.discoveriesjournals.org/discoveries/D.2019.03.DI-Kazerounian-Beggs.DOI) licensed under a CC-BY license.

C). Western Blot analysis of RPL5 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression level of RPL5 in the treated shRNA Rpl5 compared to the control mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32309614), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Rb

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-85937

DARS Antibody

(3 Publications)

0.1 ml

NBP1-85937

Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human testis.

Western Blot: DARS Antibody [NBP1-85937] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

<b>Genetic Strategies Validation. </b>Western Blot: DARS Antibody [NBP1-85937] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-DARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex, colon, kidney and testis using Anti-DARS antibody NBP1-85937 (A) shows similar protein distribution across tissues to independent antibody NBP1-86027 (B).

Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human kidney.

Immunohistochemistry-Paraffin: DARS Antibody [NBP1-85937] - Staining of human colon.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-13201

RALY Antibody

0.1 ml

NBP2-13201

Western Blot: RALY Antibody [NBP2-13201] - Analysis using Anti-RALY antibody NBP2-13201 (A) shows similar pattern to independent antibody NBP2-13200 (B).

<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: RALY Antibody [NBP2-13201] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-13201] - Staining of human colon.

Western Blot: RALY Antibody [NBP2-13201] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

<b>Genetic Strategies Validation. </b>Western Blot: RALY Antibody [NBP2-13201] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-RALY antibody. Remaining relative intensity is presented.

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-13201] - Staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-13201] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-13201] - Staining of human testis shows high expression.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RALY Antibody [NBP2-13201] - Staining in human testis and liver tissues using anti-RALY antibody. Corresponding RALY RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RALY Antibody [NBP2-13201] - Staining of human colon, liver, lymph node and testis using Anti-RALY antibody NBP2-13201 (A) shows similar protein distribution across tissues to independent antibody NBP2-13200 (B).

Immunohistochemistry-Paraffin: RALY Antibody [NBP2-13201] - Staining of human lymph node.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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