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![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0020.jpg "Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).")
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of Hela cells transiently transfected by pCMV6-ENTRY Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0011.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Ovary tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0016.jpg)
![Western Blot: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha Actinin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Alpha Actinin 1.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Western-Blot-NBP1-48251-img0018.jpg)
![Western Blot: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Western-Blot-NBP1-48251-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of HeLa cells using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunocytochemistry-Immunofluorescence-NBP1-48251-img0010.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human bladder tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0012.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human breast tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0013.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human Kidney tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0014.jpg)
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody (7A4) [NBP1-48251] - Staining of paraffin-embedded Human liver tissue using anti-Alpha Actinin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-7A4-Immunohistochemistry-Paraffin-NBP1-48251-img0015.jpg)
![Knockout Validated: alpha-Actinin 1 Antibody (OTI7A4) [NBP1-48251] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and ACTN1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ACTN1 monoclonal antibody (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-OTI7A4-Knockout-Validated-NBP1-48251-img0021.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-85791-img0011.jpg "Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-85791] - Staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85791-img0010.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-85791-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: alpha-Actinin 1 Antibody [NBP1-85791] - Analysis in human cell lines A-431 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-85791-img0009.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0013.jpg "Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: alpha-Actinin 1 Antibody [NBP1-32578] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-32578-img0007.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A:NIH-3T3.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0008.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - A: 293TB: A431 C: H1299D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0010.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0011.jpg)
![Western Blot: alpha-Actinin 1 Antibody [NBP1-32578] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha-Actinin 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-Western-Blot-NBP1-32578-img0012.jpg)
![Western Blot: alpha-Actinin 1 Antibody (3F1) [H00000087-M01] - Analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1.Lane 1: ACTN1 transfected lysate(103.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Actinin-1-Antibody-3F1-Western-Blot-H00000087-M01-img0002.jpg "Western Blot: alpha-Actinin 1 Antibody (3F1) [H00000087-M01] - Analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1.Lane 1: ACTN1 transfected lysate(103.1 KDa).Lane 2: Non-transfected lysate.")