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7 Results for "novus"

NBP2-29382

pSIVA Apoptosis Detection Kit

(1 Review)

(36 Publications)

1 Kit

NBP2-29382

Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons: NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Addition of NGF eventually rescues some neurons as shown by the loss of pSIVA-IANBD(TM) fluorescence (30h boxes).

Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying neurons. NGF withdrawal leads to cell death and pSIVA-IANBD(TM) fluorescence. Correlation of fluorescence and phase microscopy shows that NGF rescued the neurons as shown by the loss of pSIVA-IANBD(TM) fluorescence at 30h.

Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Quantitative analysis of pSIVA-IANBD™ fluorescence is rescued and NGF deprived neuronal cultures. pSIVA-IANBD(TM) fluorescence from 3 areas of the culture shows that NGF rescue (A) of dying cells was associated with loss of fluorescence whereas unrescued cells (B) continued to fluoresce.

Live Imaging Microscopy: Polarity Sensitive Indicator of Viability Apoptosis Microscopy Kit [NBP2-29382] - Rescue of dying photoreceptors in dystrophic rat neural retina. Image from verified customer review.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Alt Catalog#

IMG-6701K

Alt Catalog# IMG-6701K

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NBP1-42825

Human ESC Marker Antibody Pack

9 Vials

NBP1-42825

Live Imaging Microscopy: Human ESC Marker Antibody Pack [NBP1-42825] - Pluripotent Li-iPSCs colonies derived from reprogramming of PLCs. iPSC colonies (encircled by dashed line) were positive for the surface pluripotency marker TRA-1-60 (red) during live staining. TRA-1-60 staining (red) is shown before (C) and afteroverlaying onto the corresponding phase contrast image (D). Scale bars = 250um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221762) licensed under a CC-BY license. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]

Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. TRA-1-81 Antibody (TRA-1-81) [NB100-1833]

Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody. SSEA-3 Antibody (MC-631) [NB100-1832]

<b>Independent Antibodies Validation. </b>Immunoprecipitation: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of Mouse Nanog on HeLa whole cell lysate using NB100-588. Nanog was also IPed using NB100-587 and another Nanog. IP reactions were controlled using normal rabbit IgG. IPed Nanog was blotted using NB100-587.

Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]

Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red). SSEA-1 Antibody (MC-480) [NB100-1831]

Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.

Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of OCT4 in mouse brain lysate

Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.

Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Validated by:

Validated by:

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NBP2-81126

DRAQ7 (TM)

(1 Review)

(1 Publication)

1000 ul

NBP2-81126

Flow Cytometry: DRAQ7 (TM) [NBP2-81126] - Lymphoma cells treated with increasing quantities of staurosporine (STS). reports STS-induced apoptosis and cell death in dose-dependent manner with clear separation of positive and negative events.

Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126] - DRAQ7(TM) was added directly to unfixed THP-1 cells in RPMI + 20% FBS culture media at 3 uM (1:100) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.

Immunocytochemistry/Immunofluorescence: DRAQ7 (TM) [NBP2-81126] - Formaldehyde-fixed U2OS cells labelled with (red, nuclei+D8 and AlexaFluor 488 antibody to beta-tubulin (green).

DRAQ7 (TM) [NBP2-81126] - Spectral properties of DRAQ7 (TM) - spectral compatibility with UV-excited and most vis. Range fluorochromes for multi-colour analysis. Detection from blue excitation is achievable only by flow cytometry.

Fluorescence Imaging: DRAQ7 (TM) [NBP2-81126] - montage.

DRAQ7 (TM) [NBP2-81126] - In vitro, Cortical neurons are used to assess the glutamate toxicity using DRAQ7 dye. Image from verified customer review.

Applications:

, , ,

Applications: , , ,

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NBP2-81125

DRAQ5 (TM)

(4 Publications)

50 ul

NBP2-81125

Live Imaging Microscopy: DRAQ5 (TM) [NBP2-81125] - DRAQ5 was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.

Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - ' (red) counterstaining of fixed U2OS cells. AlexaFluor 488 antibody to beta-tubulin (green).

Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - DNA content analysis in live or fixed cells using DRAQ5 (TM)

Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - used to stain dsDNA of dividing cells.

Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - No lyse, no wash gating of nucleated cells from whole bone marrow gating on DRAQ5 (TM) signal.

DRAQ5 (TM) [NBP2-81125] - Excitation and Emission Spectra for DRAQ5 (TM).

Applications:

, , ,

Applications: , , ,

Sizes:

Sample Size Available

Sizes: Sample Size Available

Available Formulations

Formulation	Catalog #	Availability	Price
Low Concentration	NBP2-81129

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NBP2-81128

DRAQ9 (TM)

(1 Publication)

200 ul

NBP2-81128

Fluorescence Imaging: DRAQ9 (TM) [NBP2-81128] - Cells stained with .

Live Imaging Microscopy: DRAQ9 (TM) [NBP2-81128] - DRAQ9 was diluted in EMEM culture media and applied to live HeLa cells at 2 uM (1:500) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.

DRAQ9 (TM) [NBP2-81128] - Excitation and Emission Spectra for DRAQ9 (TM).

Live Imaging Microscopy: DRAQ9 (TM) [NBP2-81128] - labelling of live U2OS cells for 48 h at 2 uM - showing a number of cells in mitosis.

Fluorescence Imaging: DRAQ9 (TM) [NBP2-81128] - 6-day U2OS spheroid with continuous labeling with at 2 uM.

Applications:

, ,

Applications: , ,

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NBP2-81127

CyTRAK Orange (TM)

50 ul

NBP2-81127

Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127] - CyTRAK Orange was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.

Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127] - Nucleated cell gating by CyTRAK Orange (TM )intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.

CyTRAK Orange (TM) [NBP2-81127] - Excitation and Emission Spectra for CyTRAK Orange (TM)

Fluorescence Imaging: CyTRAK Orange (TM) [NBP2-81127] - counterstaining of fixed U2OS cells, showing differential nuclear and cytoplasmic staining.

Applications:

, , ,

Applications: , , ,

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NBP2-20982

ZNF266 Antibody

0.1 ml

NBP2-20982

Western Blot: ZNF266 Antibody [NBP2-20982] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7. 5% SDS PAGE gel, diluted at 1:1000.

Live Imaging Microscopy: ZNF266 Antibody [NBP2-20982] - ZNF266 antibody detects ZNF266 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZNF266 antibody diluted by 1:1000

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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