DRAQ7™ Summary
| Description |
Key Features of DRAQ7 (TM) :
Far-red viability dye used for investigating dead or membrane compromised cells, notably for dead cell exclusion in flow cytometry, single-cell RNAseq and GWAS
Does not enter intact, live cells and acts as an ideal replacement for propidium iodide (PI) and 7-AAD
Serves as a component of apoptosis assays, for any fluorescence-based cell analysis platform, and in cell health / in vitro toxicity assays
Rapid staining and easy to use, without the need for a wash step
Non-toxic and optimal for long-term imaging studies, up to several days
Minimal photobleaching
Spectrally compatible with GFP and FITC labels.
DRAQ7 (TM) is supplied as a blue aqueous solution and shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. |
| Purity |
>97% |
Applications/Dilutions
| Dilutions |
- Flow Cytometry 1:100
- Fluorescence Imaging 1:60 - 1:100
- Immunocytochemistry/ Immunofluorescence 1:60
- Live Imaging Microscopy 1:100
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| Application Notes |
DRAQ7 (TM) is supplied at a concentration of 0.3mM: the 250 ul size allows for 50 Flow Cytometry assays and 250 Cell Health assays whereas the 1ml size allows for 200 Flow Cytometry assays and 1,000 Cell Health assays. DRAQ7 (TM) can be diluted in culture media (e.g. RPMI 1640) and physiological buffers (eg PBS, Hankss, etc.) and mixed with fixatives such as formaldehyde. DRAQ7 (TM) has many applications in imaging, cytometry and screening and is highly compatible with existing protocols across a wide range of instrumentation platforms. |
| Reviewed Applications |
Read 2 Reviews rated 5 using
NBP2-81126 in the following applications:
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. Do not freeze. |
| Purity |
>97% |
Notes
DRAQ7 (TM) is a registered trademark of BioStatus Limited.
Background
DRAQ7 (TM) is a far-red viability dye belonging to the anthraquinone family that is closely related to DRAQ5 (TM). DRAQ7 (TM) only stains nuclei in dead and membrane-compromised (permeabilized) cells.
The excitation wavelength maxima of DRAQ7 (TM) are 600 nm / 646 nm but it can also be suboptimally excited by the 488nm (blue) laser for flow cytometry. The emission wavelength maximum of this viability dye is 697 nm when bound to dsDNA. The fluorescence signal is proportional and stoichiometric to the amount of bound DNA.
DRAQ7 (TM) is a registered trademark of BioStatus Limited.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
Publications for DRAQ7 (TM) (NBP2-81126)(1)
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Reviews for DRAQ7 (TM) (NBP2-81126) (2)
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Average Rating: 5 (Based on 2 reviews)
Reviews using NBP2-81126:
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| Images |
Ratings |
Applications |
Species |
Date |
Details |
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5
reviewed by:
Shashirekha Markandaiah
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06/06/2023 |
Summary
Comments
| Comments | In vitro, Cortical neurons are used to assess the glutamate toxicity using DRAQ7 dye. |
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5
reviewed by:
Verified Customer
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10/12/2021 |
Summary
Comments
| Comments | used at 4 micro litre per well of a 24 well dish |
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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Blogs on DRAQ7 (TM)
![Flow Cytometry: DRAQ7 (TM) [NBP2-81126] - Lymphoma cells treated with increasing quantities of staurosporine (STS). reports STS-induced apoptosis and cell death in dose-dependent manner with clear separation of positive and negative events.](http://images.novusbio.com/fullsize/DRAQ7-TM-Flow-Cytometry-NBP2-81126-img0003.jpg "Flow Cytometry: DRAQ7 (TM) [NBP2-81126] - Lymphoma cells treated with increasing quantities of staurosporine (STS). reports STS-induced apoptosis and cell death in dose-dependent manner with clear separation of positive and negative events.")
![Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126] - DRAQ7(TM) was added directly to unfixed THP-1 cells in RPMI + 20% FBS culture media at 3 uM (1:100) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.](http://images.novusbio.com/fullsize/DRAQ7-TM-Live-Imaging-Microscopy-NBP2-81126-img0005.jpg "Live Imaging Microscopy: DRAQ7 (TM) [NBP2-81126] - DRAQ7(TM) was added directly to unfixed THP-1 cells in RPMI + 20% FBS culture media at 3 uM (1:100) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.")
![Immunocytochemistry/Immunofluorescence: DRAQ7 (TM) [NBP2-81126] - Formaldehyde-fixed U2OS cells labelled with (red, nuclei+D8 and AlexaFluor 488 antibody to beta-tubulin (green).](http://images.novusbio.com/fullsize/DRAQ7-TM-Immunocytochemistry-Immunofluorescence-NBP2-81126-img0004.jpg "Immunocytochemistry/Immunofluorescence: DRAQ7 (TM) [NBP2-81126] - Formaldehyde-fixed U2OS cells labelled with (red, nuclei+D8 and AlexaFluor 488 antibody to beta-tubulin (green).")
![DRAQ7 (TM) [NBP2-81126] - Spectral properties of DRAQ7 (TM) - spectral compatibility with UV-excited and most vis. Range fluorochromes for multi-colour analysis. Detection from blue excitation is achievable only by flow cytometry.](http://images.novusbio.com/fullsize/DRAQ7-TM-NBP2-81126-img0001.jpg "DRAQ7 (TM) [NBP2-81126] - Spectral properties of DRAQ7 (TM) - spectral compatibility with UV-excited and most vis. Range fluorochromes for multi-colour analysis. Detection from blue excitation is achievable only by flow cytometry.")
![Fluorescence Imaging: DRAQ7 (TM) [NBP2-81126] - montage.](http://images.novusbio.com/fullsize/DRAQ7-TM-Immunocytochemistry-Immunofluorescence-NBP2-81126-img0002.jpg "Fluorescence Imaging: DRAQ7 (TM) [NBP2-81126] - montage.")
![DRAQ7 (TM) [NBP2-81126] - In vitro, Cortical neurons are used to assess the glutamate toxicity using DRAQ7 dye. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-81126_draq7-tm-1462023121018..jpg "DRAQ7 (TM) [NBP2-81126] - In vitro, Cortical neurons are used to assess the glutamate toxicity using DRAQ7 dye. Image from verified customer review.")
