CyTRAK Orange™ Summary
| Description |
Key Features of CyTRAK Orange (TM):
Orange fluorescent dye that preferentially stains the nucleus, but also defines the cytoplasmic area in both live and fixed preparations
Rapid staining and easy to use, without a wash or lyse step
This dual compartment label provides quick, single cell feature discrimination such as cell location, cell perimeter, cell shape and cell spread parameters
CyTRAK Orange (TM) co-excites at 488 nm with eGFP and FITC labels avoiding the need for laser alignment in confocal instruments when using this combination of probes. Since CyTRAK Orange (TM) is not excited at 633 or 647 nm, a separate series of parameters can be detected with red-exciting fluorophores such as APC, APC-Cy7.
Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. CyTRAK Orange (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution |
| Purity |
>97% |
Applications/Dilutions
| Dilutions |
- Flow Cytometry 1:500 - 1:1000
- Fluorescence Imaging 1:1000
- Immunocytochemistry/ Immunofluorescence 1:1000
- Live Imaging Microscopy 1:1000
|
| Application Notes |
CyTRAK Orange (TM) is supplied as a cranberry red aqueous solution at a concentration of 5mM: the 50 ul size covers 250 Imaging assays and 100 Flow Cytometry assays. The 200 ul size covers 1,000 Imaging assays, 6,600 HCS assays, and 400 Flow Cytometry assays. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. Do not freeze. |
| Purity |
>97% |
Notes
CyTRAK Orange (TM) is a registered trademark of BioStatus Limited.
Background
CyTRAK Orange (TM) is an orange fluorescent dye that stains the nucleus and cytoplasm but with different intensities. It functions as a DNA intercalating probe and does not crosslink with or esterify cytoplasmic proteins. Since nuclear staining is stronger than cytoplasmic staining, both cellular compartments can be easily identified during high content imaging screening. In addition CyTRAK Orange (TM) preferentially stains arrested / senescent cells compared to healthy cells, thus enabling easy identification and separation of these two distinct cellular populations.
The excitation wavelength maximum of CyTRAK Orange (TM) is 510 nm and its emission wavelength maximum is 610 nm. CyTRAK Orange (TM) is not excited by the UV or red laser.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Video Protocols
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Additional CyTRAK Orange (TM) Products
Blogs on CyTRAK Orange (TM)
![Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127] - CyTRAK Orange was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.](http://images.novusbio.com/fullsize/CyTRAK-Orange-TM-Live-Imaging-Microscopy-NBP2-81127-img0006.jpg "Live Imaging Microscopy: CyTRAK Orange (TM) [NBP2-81127] - CyTRAK Orange was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.")
![Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127] - Nucleated cell gating by CyTRAK Orange (TM )intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.](http://images.novusbio.com/fullsize/CyTRAK-Orange-TM-Flow-Cytometry-NBP2-81127-img0005.jpg "Flow Cytometry: CyTRAK Orange (TM) [NBP2-81127] - Nucleated cell gating by CyTRAK Orange (TM )intensity of intact, unlysed bone marrow with retained forward and side scatter characteristics.")
![CyTRAK Orange (TM) [NBP2-81127] - Excitation and Emission Spectra for CyTRAK Orange (TM)](http://images.novusbio.com/fullsize/CyTRAK-Orange-TM-NBP2-81127-img0001.jpg "CyTRAK Orange (TM) [NBP2-81127] - Excitation and Emission Spectra for CyTRAK Orange (TM)")
![Fluorescence Imaging: CyTRAK Orange (TM) [NBP2-81127] - counterstaining of fixed U2OS cells, showing differential nuclear and cytoplasmic staining.](http://images.novusbio.com/fullsize/CyTRAK-Orange-TM-Immunocytochemistry-Immunofluorescence-NBP2-81127-img0002.jpg "Fluorescence Imaging: CyTRAK Orange (TM) [NBP2-81127] - counterstaining of fixed U2OS cells, showing differential nuclear and cytoplasmic staining.")
![CyTRAK Orange (TM) [NBP2-81127] - Absorbance profile of CyTRAK Orange (TM)compared to eGFP and APC.](http://images.novusbio.com/fullsize/CyTRAK-Orange-TM-NBP2-81127-img0003.jpg "CyTRAK Orange (TM) [NBP2-81127] - Absorbance profile of CyTRAK Orange (TM)compared to eGFP and APC.")
![CyTRAK Orange (TM) [NBP2-81127] - Emission profile of CyTRAK Orange (TM)compared to eGFP and Cy7](http://images.novusbio.com/fullsize/CyTRAK-Orange-TM-NBP2-81127-img0004.jpg "CyTRAK Orange (TM) [NBP2-81127] - Emission profile of CyTRAK Orange (TM)compared to eGFP and Cy7")
