Live Imaging Microscopy: DRAQ5 (TM) [NBP2-81125] - DRAQ5 was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done ...read more
Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - ' (red) counterstaining of fixed U2OS cells. AlexaFluor 488 antibody to beta-tubulin (green).
Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - DNA content analysis in live or fixed cells using DRAQ5 (TM)
Immunocytochemistry/ Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - used to stain dsDNA of dividing cells.
Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - No lyse, no wash gating of nucleated cells from whole bone marrow gating on DRAQ5 (TM) signal.
DRAQ5 (TM) [NBP2-81125] - Excitation and Emission Spectra for DRAQ5 (TM).
Bright, far-red DNA dye that works on live or fixed cell and tissue preparations for multiple applications such as flow cytometry, fluorescence imaging, and high content screening
Proven no-lyse, no-wash method for measuring DNA content in cells
Tested on hundreds of cell and tissue types
Exceptionally photostable so no photobleaching effect
Convenient, ready-to-use formulation
Enables phenotyping of blood/BM and direct cellular DNA content analysis in flow cytometry without any RBC lysis or fixation, permeabilization or RNase treatment
Spectrally compatible with most visible-range fluorophores including GFP/FITC and R-PE
Products are shipped at ambient temperature, but on receipt packs should be stored at 2-8C. DO NOT FREEZE. DRAQ5 (TM) can come out of solution when frozen and it is difficult (but not impossible) to get it back into solution.
Purity
>97%
Applications/Dilutions
Dilutions
Flow Cytometry 1:250 - 1:1000
Fluorescence Imaging 1:1000
Immunocytochemistry/ Immunofluorescence 1:1000
Live Imaging Microscopy 1:1000
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:34354753).
Publications
Read Publications using NBP2-81125 in the following applications:
DRAQ5 (TM) is a registered trademark of BioStatus Limited.
Alternate Names for DRAQ5 (TM)
DRAQ5
Background
Deep Red Anthraquinone 5 (DRAQ5 (TM)) is a cell-permeable dye belonging to the anthraquinone family that intercalates with high affinity to double stranded DNA (dsDNA). This far-red emitting fluorescent DNA dye is used for cell cycle analysis and nuclear staining. No measurable binding to mitochondrial DNA (mtDNA) has been detected and DRAQ5 (TM) shows negligible binding to RNA.
The excitation wavelength maxima of DRAQ5 (TM) are 600 nm / 646 nm but it can also be suboptimally excited by the 488nm (blue laser) for flow cytometry. Its emission wavelength maximum is 697 nm when intercalated with dsDNA. The fluorescence signal is proportional and stoichiometric to the quantity of nuclear DNA present in the cell.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Publications for DRAQ5 (TM) (NBP2-81125)(4)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: FLOW, ICC/IF.
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Showing Publications 1 - 4 of 4.
Publications using NBP2-81125
Applications
Species
Sota S Myeloid MDA5-MAVS Sensing Demarcates Acute to Persistent Phase Transition During Murine Norovirus Infection Thesis 2023-01-01
![Live Imaging Microscopy: DRAQ5 (TM) [NBP2-81125] - DRAQ5 was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.](http://images.novusbio.com/fullsize/DRAQ5-TM-Live-Imaging-Microscopy-NBP2-81125-img0007.jpg "Live Imaging Microscopy: DRAQ5 (TM) [NBP2-81125] - DRAQ5 was diluted in EMEM culture media and applied to live HeLa cells at 5 uM (1:1000) for 30 minutes at room temperature and protected from light. Imaging was done immediately after staining without washing the cells.")
![Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - ' (red) counterstaining of fixed U2OS cells. AlexaFluor 488 antibody to beta-tubulin (green).](http://images.novusbio.com/fullsize/DRAQ5-TM-Immunocytochemistry-Immunofluorescence-NBP2-81125-img0003.jpg "Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - ' (red) counterstaining of fixed U2OS cells. AlexaFluor 488 antibody to beta-tubulin (green).")
![Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - DNA content analysis in live or fixed cells using DRAQ5 (TM)](http://images.novusbio.com/fullsize/DRAQ5-TM-Flow-Cytometry-NBP2-81125-img0006.jpg "Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - DNA content analysis in live or fixed cells using DRAQ5 (TM)")
![Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - used to stain dsDNA of dividing cells.](http://images.novusbio.com/fullsize/DRAQ5-TM-Immunocytochemistry-Immunofluorescence-NBP2-81125-img0004.jpg "Immunocytochemistry/Immunofluorescence: DRAQ5 (TM) [NBP2-81125] - used to stain dsDNA of dividing cells.")
![Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - No lyse, no wash gating of nucleated cells from whole bone marrow gating on DRAQ5 (TM) signal.](http://images.novusbio.com/fullsize/DRAQ5-TM-Flow-Cytometry-NBP2-81125-img0005.jpg "Flow Cytometry: DRAQ5 (TM) [NBP2-81125] - No lyse, no wash gating of nucleated cells from whole bone marrow gating on DRAQ5 (TM) signal.")
![DRAQ5 (TM) [NBP2-81125] - Excitation and Emission Spectra for DRAQ5 (TM).](http://images.novusbio.com/fullsize/DRAQ5-TM-NBP2-81125-img0001.jpg "DRAQ5 (TM) [NBP2-81125] - Excitation and Emission Spectra for DRAQ5 (TM).")
