| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NB100-56726 | |||
| BSA Free | NB100-56722SS | |||
| Azide Free | NB100-56726SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB100-56727 | |||
| BSA Free | NB100-56723SS | |||
| Azide and BSA Free | NB100-56727SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-206-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB555-SP | |||
| Carrier Free | MAB555-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB510-SP | |||
| Carrier Free | MAB510-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2171-SP | |||
| Carrier Free | MAB2171-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB529-SP | |||
| Carrier Free | MAB529-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80038-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1167-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB356A | |||
| Carrier Free | MAB356-SP | |||
| Carrier Free | MAB356-500 | |||
| Carrier Free | FAB356V-100UG | |||
| Carrier Free | FAB356U-100UG | |||
| Carrier Free | FAB356T-100UG | |||
| Carrier Free | FAB356S-100UG | |||
| Carrier Free | FAB356R-100UG | |||
| Carrier Free | FAB356N-100UG | |||
| Carrier Free | FAB356G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF260-SP | |||
![Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Flow-Cytometry-NB100-56722-img0005.jpg "Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Analysis using the FITC conjugate of NB100-56726. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.")
![Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Expression of TLR2 was determined by western blotting. The immunoreactivity of the anti-TLR2 was confirmed with human intestinal lysate. Data are representative for analysis of >=2 independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0060671) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Western-Blot-NB100-56722-img0015.jpg)
![Western Blot: TLR2 Antibody (TL2.1) [NB100-56722] - Total protein from human THP-1 and U937 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TLR2 (NB100-56722) in 5% BSA-TBST and detected with an anti-mouse HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Western-Blot-NB100-56722-img0016.jpg)
![Flow Cytometry: TLR2 Antibody (TL2.1) [NB100-56722] - Monitoring of TLR2 expression in normal B cells and primary MCL cells cultured for 24h in the absence/presence of CD40L, IL-4, and TLR ligands. Filled curves refer to isotypic control, empty curves to the TLR2 antibody (NB100-56722) of untreated samples. Dotted histograms represent CD40L+IL4 activated cells, dashed curves indicate CD40L+IL4 activated cells further treated with TLR ligands. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0153823) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Flow-Cytometry-NB100-56722-img0013.jpg)
![Flow (Cell Surface): TLR2 Antibody (TL2.1) [NB100-56722] - Flow (Cell Surface): - Azide Free [NB100-56726] - Analysis using the PE conjugate of NB100-56726. Staining of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green: isotype control. Red: anti-TLR2 antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Flow-Cell-Surface-NB100-56722-img0010.jpg)
![Block/Neutralize: TLR2 Antibody (TL2.1) [NB100-56722] - Human monocyte-derived DCs were pre-incubated wit TLR-neutralizing antibodies including TLR2 antibody (NB100-56722) for 1 h. Afterwards, DCs were infected with H. pylori G27 (MOI 5) and the levels of the costimulatory molecule CD86 and the maturation marker CD83 were analyzed by FACS on CD11c+ cells 24 h post-infection. Data are presented as mean +/- SD of five independent experiments. *p<=0.05, **p<=0.005, ***p<=0.0005. Asterisks on top of bars indicate significance relative to non-neutralized, H. pylori-primed control cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104804) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR2-Antibody-TL2-1-Block-Neutralize-NB100-56722-img0014.jpg)
![Western Blot: TLR4 Antibody (HTA125) [NB100-56723] - Untransfected 293T cells probed with the monoclonal antibody against TLR4 (NB100-56723, Lane 1), TLR4 transfected 293T cells probed with the TLR4 monoclonal antibody (Lane 2), and with antibodies affinity-purified against the celiac peptide (Lane 3). TLR4 transfected 293T cells probed with TLR4 monoclonal (biotin) antibody (Lane 4) and probed first with affinity-purified anti-peptide antibodies, followed by an incubation with biotin-labelled anti-TLR4 monoclonal antibody (Lane 5). Human plasmocytoid dendritic cells probed with TLR4 monoclonal antibody (Lane 6), human monocytes probed with TLR4 monoclonal antibody (Lane 7) and with affinity-purified anti-peptide antibodies (Lane 8). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pmed.0030358) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-HTA125-Western-Blot-NB100-56723-img0023.jpg "Western Blot: TLR4 Antibody (HTA125) [NB100-56723] - Untransfected 293T cells probed with the monoclonal antibody against TLR4 (NB100-56723, Lane 1), TLR4 transfected 293T cells probed with the TLR4 monoclonal antibody (Lane 2), and with antibodies affinity-purified against the celiac peptide (Lane 3). TLR4 transfected 293T cells probed with TLR4 monoclonal (biotin) antibody (Lane 4) and probed first with affinity-purified anti-peptide antibodies, followed by an incubation with biotin-labelled anti-TLR4 monoclonal antibody (Lane 5). Human plasmocytoid dendritic cells probed with TLR4 monoclonal antibody (Lane 6), human monocytes probed with TLR4 monoclonal antibody (Lane 7) and with affinity-purified anti-peptide antibodies (Lane 8). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pmed.0030358) licensed under a CC-BY license.")
![Block/Neutralize: TLR4 Antibody (HTA125) [NB100-56723] - Human monocyte-derived DCs were pre-incubated wit TLR-neutralizing antibodies including TLR4 antibody (NB100-56723) for 1 h. Afterwards, DCs were infected with H. pylori G27 (MOI 5) and the levels of the costimulatory molecule CD86 and the maturation marker CD83 were analyzed by FACS on CD11c+ cells 24 h post-infection. Data are presented as mean +/- SD of five independent experiments. *p<=0.05, **p<=0.005, ***p<=0.0005. Asterisks on top of bars indicate significance relative to non-neutralized, H. pylori-primed control cells. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0104804) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR4-Antibody-HTA125-Block-Neutralize-NB100-56723-img0022.jpg)
![Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody. Secondary antibody goat anti-mouse IgG-PE at 0.25ug/10^6 cells. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were equally stained.](http://images.novusbio.com/fullsize/TLR4-Antibody-HTA125-Flow-Cytometry-NB100-56723-img0011.jpg)
![Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Staining of U937 cells with mouse anti human CD284:RPE. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-HTA125-Flow-Cytometry-NB100-56723-img0016.jpg)
![Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Staining of human peripheral blood monocytes with Mouse anti Human CD284.](http://images.novusbio.com/fullsize/TLR4-Antibody-HTA125-Flow-Cytometry-NB100-56723-img0014.jpg)
![Block/Neutralize: TLR4 Antibody (HTA125) [NB100-56723] - Validation of the neutralizing effect of this antibody on TLR4 activity. The TLR4/MD-2/CD14/IL-8 Prom/LUCPorter HeLa cells were plated in 96-well white plates at 1 x 10^5 cells/well. After 16 h, cells were preincubated with different amounts of this antibody for 1 h and then stimulated with 10 ng/ml LPS for 6 h. Luciferase activity was then directly analyzed using the one-step luciferase reporter assay reagent. Data Summary: It inhibited LPS-mediated TLR4 activity in the TLR4/MD-2/CD14/IL-8 Prom/LUCPorter cell line. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TLR4-Antibody-HTA125-Block-Neutralize-NB100-56723-img0020.jpg)
![IL-10 receptors expressed on hippocampal neurons.(A) Expression of IL-10 receptor mRNAs in hippocampal neurons. The expression of the IL-10 receptor was identified using RT-PCR. The mRNAs of IL-10 receptor alpha and beta were expressed in the hippocampal neurons. IL-10 receptor alpha was expressed mainly in hippocampal neurons of DIV 7. (1, cultured hippocampal neurons at DIV 7; 2, cultured hippocampal neurons at DIV 15; 3, mixed glial culture at DIV 7; 4, mixed glial culture at DIV 15; 5, microglia) Quantification (DIV 15 neuron/ DIV 7 neuron): IL-10 receptor alpha , 0.61; IL-10 receptor beta , 1.06. (B) Expression of IL-10 receptor proteins in cultured hippocampal neurons. Similar to the expression of mRNA, the IL-10 receptor alpha protein was expressed mainly in neurons of DIV 7. Anti-IL-10 receptor alpha antibodies (0.5 μg/ml, Santa Cruz, sc-985) were used for western blotting [27]. Quantification (DIV 15 neuron/ DIV 7 neurons): IL-10 receptor alpha , 0.73.(C) Expression of IL-10 receptor proteins in the developing rat brains. The IL-10 receptor alpha proteins were expressed mainly in the developing brains of embryonic and early postnatal days (E18~P3). Quantification of IL-10 receptor alpha : E18, 0.30; P1, 0.27; P3, 1.0; P7, 0.22; P14, 0.20; P3W, 0.17; P6W, 0.15 (E, embryonic days; P, postnatal days).(D) Images of IL-10 receptor expressions in cultured hippocampal neurons. Hippocampal neurons of DIV 6 were stained with antibodies of IL-10 receptor alpha (5 μg/ml, Santa Cruz, sc-985) (red) and MAP2 (the neuronal marker, green) after treatment with 4% formaldehyde and then -20 °C methanol. Hippocampal neurons expressed IL-10 receptor proteins comparable to spinal neurons or cortical neurons.(E) The induction of synaptic formation by microglia was antagonized by the neutralizing antibody of IL-10 receptor alpha . When anti-mouse IL-10 receptor alpha antibody was applied to the co-culture of mouse microglia and mouse hippocampal neurons, the density of dendritic spines was significantly decreased compared with the control (without anti-IL-10 receptor antibody). Means±SEM. n=30 dendrites for no microglia without anti-IL-10 receptor antibody, 29 for the application of microglia only, 29 for no microglia with anti-IL-10 receptor antibody only, 29 for microglia with anti-IL-10 receptor antibody. ***p<0.001 by the Newman-Keuls multiple comparison test after application of one-way ANOVA, F=17.35, p<0.0001.(F) The induction of synaptic formation via microglia was not antagonized by the blocking antibody of TNF alpha . When anti-rat TNF alpha antibody was applied to the co-culture of rat microglia and rat hippocampal neurons, the density of dendritic spine was not decreased compared with control (without anti-TNF alpha antibody). Means±SEM. n=27 dendrites for no microglia without anti-TNF alpha antibody, 28 for the application of microglia only, 29 for no microglia with anti-TNF alpha receptor antibody only, 27 for microglia with anti-TNF alpha antibody. **p<0.01 and ***p<0.001 by the Newman-Keuls multiple comparison test after application of one-way ANOVA, F=9.104, p<0.0001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24278397), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab510_rat-tnf-alpha-mab-clone-45418-41202412511313.jpg)
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0003.jpg "Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 1ug/mL Fc-tagged recombinant human Spike S1 subunit RBD is shown to bind Ace-2 (black dotted line) detected by Human IgG Fc-APC Secondary Antibody (FAB110A). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse Anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).")
![Block/Neutralize: ACE-2 Antibody (AC384) [NBP2-80038] - In a functional flow cytometry assay using a HEK293 human embryonic kidney cell line transfected with human ACE-2, 0.5 ug/mL sheep Fc-tagged recombinant human Spike S1 is shown to bind ACE-2 (black dotted line) detected by Donkey anti-Sheep APC Secondary Antibody (Catalog # F0127). Binding is completely blocked (orange histogram) by 25 ug/mL of Mouse Anti-Human ACE-2 Monoclonal Antibody (NBP2-80038). 25 ug/mL Mouse anti-Human DC-SIGN/CD209 Monoclonal Antibody (MAB161) was used as an irrelevant control (blue line).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0004.jpg)
![Western Blot: ACE-2 Antibody (AC384) [NBP2-80038] - 1: Human ACE2 Fc-fusion protein. 2: Human ACE2 (Ecto domain) (FLAG®-tagged). 3: HepG2 cell lysate. 4: Other Fc-fusion (hGITR-Fc) proteins (negative control). 1:2'000 dilution.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Western-Blot-NBP2-80038-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 staining in paraffin-embedded sections of hamster lung tissue using Mouse Anti-Human ACE-2 (AC384) Monoclonal Antibody (Catalog # NBP2-80038) at 10 ug/ml overnight at 4 degrees C. Detection kit: VC001. Counterstain: Hematoxylin. Localization: respiratory bronchioles.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0007.jpg)
![Flow Cytometry: ACE-2 Antibody (AC384) [NBP2-80038] - Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human ACE-2 Monoclonal Antibody (Catalog # NBP2-80038) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 control antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Flow-Cytometry-NBP2-80038-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (AC384) [NBP2-80038] - ACE-2 was detected in immersion fixed paraffin-embedded sections of human lung normal (left) and lung cancer tissue (right) using Mouse Anti-HumanACE-2 (AC18F) Monoclonal Antibody (Catalog # NBP2-80038) at 15ug/mL for 1 hour at room temperature followed by incubation with the Goat anti-Mouse IgG Secondary Antibody.](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Immunohistochemistry-Paraffin-NBP2-80038-img0006.jpg)
![Functional: ACE-2 Antibody (AC384) [NBP2-80038] - Binding of ACE2 (human) to the Spike protein of SARS-CoV-2 is inhibited by the antibody anti-ACE2 (human), mAb (blocking) (AC384). Spike (SARS-CoV-2):Fc (human) (RBD) (rec.) is coated on an ELISA plate at 1ug/ml. ACE2 (human), mAb (blocking) (AC384) or an unrelated mAb Control are added (starting at 40 ug/mL with a two-fold serial dilution) together with 500 ng/uL of ACE2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).](http://images.novusbio.com/fullsize/ACE-2-Antibody-AC384-Functional-NBP2-80038-img0001.jpg)
