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NBP1-30042

Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free

(2 Reviews)

(36 Publications)

0.1 ml

NBP1-30042

Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Western blot analyses of periostin from soleus muscle. The graph shows quantification of periostin levels normalized to GAPDH (n = 4). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Skeletal muscle pathology of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from soleus muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.

Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.

Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.

Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age. Immunostaining of laminin a2 (green) and periostin (red) with DAPI (blue) from cardiac muscle. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0221512), licensed under a CC-BY license.

<b>Genetic Strategies Validation. </b>Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Representative IHC images from 3 separate hearts analyzed for Periostin/OSF-2 protein expression within the infarct region of hearts from Postn^MCM/+; R26^EGFP mice versus Postn^MCM/MCM; R26^EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 20 um. Postn lineage-traced cells (EGFP+) cells are also shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29664017/) licensed under a CC-BY license.

Knockout Validated: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Loss of Tgfbi does not alter disease progression after MI. Western blot analysis for TGFBI and periostin from isolated infarct areas of the hearts of the indicated groups of mice 7 days after MI. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0181945), licensed under a CC-BY license.

Effect of ECM maturation on alphaSMA expression in myofibroblasts. (D) Representative IHC images from 3 separate hearts analyzed for Postn protein expression within the infarct region of hearts from PostnMCM/+;R26EGFP mice versus PostnMCM/MCM;R26EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 20 um. Postn lineage-traced cells (EGFP+) cells are also shown. (E) Representative IHC images from 3 separate hearts analyzed showing alphaSMA+ (red) and Postn lineage-traced (EGFP+) fibroblasts in the infarct region of hearts from PostnMCM/+;R26EGFP mice and PostnMCM/MCM;R26EGFP mice 2 weeks after MI. Nuclei are shown with DAPI (blue). Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29664017), licensed under a CC-BY licence.

Selective addiction of EOC to the SIX4 homeobox transcription factorImmunoblots indicate miR‐124‐induced SIX4 depletion. siSIX4 is shown as an antibody control.Consequence of SIX4 depletion on EOC cell viability (as in Fig 2A, N, normal cell lines: HOSE, HBEC30).In vivo knockdown of SIX4 using neutral liposome‐incorporated siRNA reduced tumor burden and ascites volume in an orthotopic xenograft mouse model. Box‐and‐whisker plot of tumor weight (left panel) or ascites volume (right panel) from n = 7 or 8 mice per condition as indicated. *P‐value from Student's t‐test.Consequence of SIX4 depletion in PEO1 cells on E‐cadherin plasma membrane accumulation 48 h after transfection. Cells were counterstained with phalloidin and DAPI.Immunoblots indicate consequence of SIX4 depletion on LKB1 accumulation and AMPK pathway activation.Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26655797), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Both regorafenib and SC-78 targeted autocrine and paracrine regulation of VEGF-A in TNBCs.(A) Cells were treated with SC-78 or regorafenib for 24 h. After that, the medium was replaced with fresh medium and incubated for another 24 h. Then, the medium was collected and ELISA was used to measure the VEGF-A level in the CM harvested from TNBC cells w/wo treatment of SC-78 or regorafenib. *p < 0.05, **p < 0.01. (B) Top, For IP-western assay, MDA-MB-231 cells were incubated with CM for 24 h and total lysate was immunoprecipitated using anti-NRP-1 antibody, and western blotting was performed using the indicated antibodies. Bottom, the methods to collect CM described above were followed, and the VEGF165 recombinant protein was re-added into CM harvested from MDA-MB-231 cells w/wo treatment of SC-78 or regorafenib for 24 h, then cells were further analyzed by IP-western assay. (C) The co-localization of VEGF-A (green) and NRP-1 (red) in MDA-MB-231 cells treated with CM harvested from MDA-MB-231cells w/wo treatment of SC-78 or regorafenib was examined under a confocal microscope after immunofluorescent staining. DAPI stains the nuclei. (D) Left, MDA-MB-231 cells were seeded and treated with VEGF165 at the indicated concentration (upper chamber of the Transwell plate). The migrated cells were stained with DAPI and counted after 18 h by immunofluorescence microscope. MDA-MB-231 cells were exposed to regorafenib (middle) or SC-78 (right) w/wo the addition of VEGF165. Cells were subjected to Transwell migration assay as mentioned above. *p < 0.05, **p < 0.01. (E) CM was harvested from TNBC cells w/wo treatment of regorafenib or SC-78. EA.hy926 cells (5 × 104) in CM were seeded and tube-formation ability was examined after incubation for 24 h. (F) For DIVAA, mice were implanted with silicone tubes containing Matrigel and supplemented with angiogenic factors and CM harvested from cells treated with various doses of regorafenib or SC-78 (n = 3). Neovascularization in the angioreactors was quantified using a spectrofluorometer 14 days after implantation. *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27364975), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Analysis of phosphorylated P-IKK alpha , and human and mouse IKK alpha in tumors of the three groupsA, D, G. P-IKK alpha expression. P-IKK alpha / beta (Ser 180/Ser 181) antibody is used. B, E, H. Specific staining of human IKK alpha -using the NB100-56704 antibody. C, F, I. Staining with the sc-7182 antibody that recognizes both human and mouse IKK alpha . Observe that as expected, in the N-IKK alpha tumors the signal of this antibody is detected both in cytoplasmic and nuclear localization; by contrast, in the Control tumors the endogenous IKK alpha is mainly observed in the cytosolic compartment, although some nuclear staining is also observed. In the C-IKK alpha tumors little nuclear staining is observed. Scale bar: 70μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27121058), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ER stress is induced by OGR1 via IRE1 alpha /JNK signalling. (A) Caco-2 cells were subjected to different pH medium, with or without an OGR1 inhibitor (10 µM), following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated and Western blot performed. Results are representative of two independent experiments. (B) Caco-2 cells were subjected to different pH medium After 24 h pH shift, total protein was isolated and Western blot performed. Results are representative of two independent experiments. (C) Caco-2 cells were subjected to different pH medium with or without a JNK inhibitor (10 µM) following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated and Western blot performed. Results are representative of two independent experiments. (D) Caco-2 cells were starved and subjected to an acidic pH with or without a JNK inhibitor as described in (C). After 24 h pH shift, total RNA was isolated and mRNA expression was investigated by qPCR. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Data are presented as means ± SE of three independent experiments (***p < 0.001). (E) Caco-2 cells were starved and subjected to different pH medium following 4–6 h in pH 7.6 serum free medium. After 24 h pH shift, total protein was isolated and co-IP using IRE1 alpha antibody and JNK antibody was performed, followed by immunoblotting. Results are representative of two independent experiments. pH conditions: High pH 7.5–7.8; Normal pH 7.2–7.4; Low pH 6.6–6.8. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31996710), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Thapsigargin treatment induces calpain activation and autophagy. (A) Control and shCAPNS1 cells were treated with 100 nM thapsigargin for 10, 20 and 30 min and the lysates subjected to western blot analysis to quantify the precursor and active form of CAPN1. The ratio between active CAPN1 and tubulin is reported below each lane. (B) Time course measurement of intracellular calcium concentration. The plot indicates the ratio of mean Indo Violet/Indo Blue emission values of each cell population at individual time points (minutes). The ratio corresponds to the relative calcium concentration of control and shCAPNS1 U2OS cells before and after addition of thapsigargin (100 nm final). Moving average was used as smoothing method. (C) shCAPNS1 cells were treated with 100 nM thapsigargin for 30, 60 and 120 min and the lysates subjected to western blot analysis to detect LC3, p62, pIRE, CAPNS1 and tubulin. (D) Levels of p62 and LC3 normalized to tubulin levels. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/bio/article/doi/10.1242/bio.022806/256614/Calpain-mobilizes-Atg9-Bif-1-vesicles-from-Golgi), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

DSC restores redox balance in colonic tissues. A, Colitis was induced as described in Materials and Methods and treated with or without DSC (50 mg/kg). Representative bands and densitometry analysis of Nrf2 nuclear translocation and HO‐1 expression in colonic tissues. Histone H3 and beta ‐actin were used as loading control, respectively. B, Colitis was induced as described in Materials and Methods and treated with lentiviral Nox4 shRNA or lentiviral scrambled shRNA. Representative bands and densitometry analysis of Nrf2 nuclear translocation and HO‐1 expression in colonic tissues. Histone H3 and beta ‐actin were used as loading control, respectively. BMDM was stimulated with or without DSC (50 μmol/L) as described in Materials and Methods. C, Representative bands and densitometry analysis of Nox4. beta ‐actin was used as loading control. D, Representative bands and densitometry analysis of Nrf2 nuclear translocation and cytoplasmic HO‐1 expression in BMDM. beta ‐actin or histone H3 was used as loading control. Data shown are means ± SEM of n = 8 in each group. *P < 0.05 vs Control cell or mice (CTL), #P < 0.05 vs DSS‐treated mice or LPS‐stimulated cells Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32945118), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ch, Bv, Ca, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Bv, Ca, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Sh

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP1-30042SS

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NBP1-82472

Periostin/OSF-2 Antibody

(7 Publications)

0.1 ml

NBP1-82472

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining in human skin and pancreas tissues using anti-POSTN antibody. Corresponding POSTN RNA-seq data are presented for the same tissues.

<b>Orthogonal Strategies Validation. </b>Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human skin shows high expression.

<b>Genetic Strategies Validation. </b>Knockdown Validated: Periostin/OSF-2 Antibody [NBP1-82472] - Characteristics of lung cancer cell lines according to Periostin/OSF-2 (POSTN) expression. Comparison of Periostin/OSF-2 expression levels detected by Western blot. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/23/3/1240) licensed under a CC-BY license.

Immunohistochemistry: Periostin/OSF-2 Antibody [NBP1-82472] - Section of non-small cell lung carcinoma (NSCLC) immunostained for Periostin/OSF-2 in cancer-associated fibroblasts (CAFs). Magnification 50×. Bar = 200 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32987711/), licensed under a CC-BY license.

Serial sections of non-small cell lung carcinoma (NSCLC) immunostained for (A) periostin (POSTN), (B) alpha -smooth muscle actin ( alpha -SMA), (C) podoplanin (D2-40), and (D) vimentin in cancer-associated fibroblasts (CAFs). Magnification 50×. Bar = 200 µm.

Reactivity:

Hu, Ca, Mu, Rt

Species Glossary

Reactivity: Hu, Ca, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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AF3548

Periostin/OSF-2 Antibody [Unconjugated]

(5 Publications)

100 μg

AF3548

Human Periostin/OSF-2 was immunoprecipitated from human milk samples diluted in 1X Sample Diluent Concentrate 2 (DYC002) and incubated with 3 µg Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) or Normal Goat IgG Control (AB-108-C) plus 30 µL Protein G beads overnight. Immunoprecipitated Periostin/OSF-2 was detected by Western blot under reducing conditions using 1 µg/mL Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548). View our recommended buffer recipes for immunoprecipitation.

Periostin/OSF-2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of Human Breast Cancer. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Periostin/OSF‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Periostin/OSF‑2 at approximately ~75kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3548

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3548-SP

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NBP2-45371

Periostin/OSF-2 Antibody (OTI2B2)

0.1 ml

NBP2-45371

Western Blot: Periostin/OSF-2 Antibody (OTI2B2) [NBP2-45371] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Periostin.

Immunohistochemistry: Periostin/OSF-2 Antibody (OTI2B2) [NBP2-45371] - Staining for POSTN protein of negative control, positive control,enchondroma, and low grade chondrosarcoma (original magnification x 400) by using anti-POSTN antibody. Dilution: 1:1000

Immunohistochemistry: Periostin/OSF-2 Antibody (OTI2B2) [NBP2-45371] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: Periostin/OSF-2 Antibody (OTI2B2) [NBP2-45371] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73336AF350
Alexa Fluor 405	NBP2-73336AF405
Alexa Fluor 488	NBP2-73336AF488
Alexa Fluor 532	NBP2-73336AF532
Alexa Fluor 594	NBP2-73336AF594
Alexa Fluor 647	NBP2-73336AF647
Alexa Fluor 700	NBP2-73336AF700
Alexa Fluor 750	NBP2-73336AF750
Allophycocyanin	NBP2-73336APC
Biotin	NBP2-73336B
DyLight 350	NBP2-73336UV
DyLight 405	NBP2-73336V
DyLight 488	NBP2-73336G
DyLight 550	NBP2-73336R
DyLight 594	NBP2-73336DL594
DyLight 650	NBP2-73336C
DyLight 680	NBP2-73336FR
DyLight 755	NBP2-73336IR
FITC	NBP2-73336F
HRP	NBP2-73336H
Janelia Fluor 549	NBP2-73336JF549
Janelia Fluor 646	NBP2-73336JF646
PE	NBP2-73336PE
PerCP	NBP2-73336PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73336

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