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Periostin/OSF-2 Antibody (OTI2B2) - Azide and BSA Free

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Western Blot: Periostin/OSF-2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73336] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Periostin.
Western Blot: Periostin/OSF-2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73336] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Periostin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73336AF350
Alexa Fluor 405
NBP2-73336AF405
Alexa Fluor 488
NBP2-73336AF488
Alexa Fluor 532
NBP2-73336AF532
Alexa Fluor 594
NBP2-73336AF594
Alexa Fluor 647
NBP2-73336AF647
Alexa Fluor 700
NBP2-73336AF700
Alexa Fluor 750
NBP2-73336AF750
Allophycocyanin
NBP2-73336APC
Biotin
NBP2-73336B
DyLight 350
NBP2-73336UV
DyLight 405
NBP2-73336V
DyLight 488
NBP2-73336G
DyLight 550
NBP2-73336R
DyLight 594
NBP2-73336DL594
DyLight 650
NBP2-73336C
DyLight 680
NBP2-73336FR
DyLight 755
NBP2-73336IR
FITC
NBP2-73336F
HRP
NBP2-73336H
Janelia Fluor 549
NBP2-73336JF549
Janelia Fluor 646
NBP2-73336JF646
PE
NBP2-73336PE
PerCP
NBP2-73336PCP
Unconjugated
NBP2-45371
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45371
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Periostin/OSF-2 Antibody (OTI2B2) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 538-781 of human POSTN (NP_001129407) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POSTN
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
87.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Periostin/OSF-2 Antibody (OTI2B2) - Azide and BSA Free

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Periostin/OSF-2 Antibody (NBP2-73336) (0)

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol POSTN