| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-171-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80769 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1023AF405 | |||
| Alexa Fluor 488 | NB21-1023AF488 | |||
| Alexa Fluor 647 | NB21-1023AF647 | |||
| Alexa Fluor 700 | NB21-1023AF700 | |||
| Biotin | NB21-1023B | |||
| DyLight 405 | NB21-1023V | |||
| DyLight 488 | NB21-1023G | |||
| DyLight 550 | NB21-1023R | |||
| DyLight 650 | NB21-1023C | |||
| FITC | NB21-1023F | |||
| HRP | NB21-1023H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1023SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1091AF405 | |||
| Alexa Fluor 488 | NB21-1091AF488 | |||
| Alexa Fluor 647 | NB21-1091AF647 | |||
| Alexa Fluor 700 | NB21-1091AF700 | |||
| Biotin | NB21-1091B | |||
| DyLight 405 | NB21-1091V | |||
| DyLight 488 | NB21-1091G | |||
| DyLight 550 | NB21-1091R | |||
| DyLight 650 | NB21-1091C | |||
| FITC | NB21-1091F | |||
| HRP | NB21-1091H | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1022AF405 | |||
| Alexa Fluor 488 | NB21-1022AF488 | |||
| Alexa Fluor 647 | NB21-1022AF647 | |||
| Alexa Fluor 700 | NB21-1022AF700 | |||
| Biotin | NB21-1022B | |||
| DyLight 405 | NB21-1022V | |||
| DyLight 488 | NB21-1022G | |||
| DyLight 550 | NB21-1022R | |||
| DyLight 650 | NB21-1022C | |||
| FITC | NB21-1022F | |||
| HRP | NB21-1022H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1022SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36468SS | |||
| Azide and BSA Free | NBP2-80770 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1024AF405 | |||
| Alexa Fluor 488 | NB21-1024AF488 | |||
| Alexa Fluor 647 | NB21-1024AF647 | |||
| Alexa Fluor 700 | NB21-1024AF700 | |||
| Biotin | NB21-1024B | |||
| DyLight 405 | NB21-1024V | |||
| DyLight 488 | NB21-1024G | |||
| DyLight 550 | NB21-1024R | |||
| DyLight 650 | NB21-1024C | |||
| FITC | NB21-1024F | |||
| HRP | NB21-1024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1024SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1021AF405 | |||
| Alexa Fluor 488 | NB21-1021AF488 | |||
| Alexa Fluor 647 | NB21-1021AF647 | |||
| Alexa Fluor 700 | NB21-1021AF700 | |||
| Biotin | NB21-1021B | |||
| DyLight 405 | NB21-1021V | |||
| DyLight 488 | NB21-1021G | |||
| DyLight 550 | NB21-1021R | |||
| DyLight 650 | NB21-1021C | |||
| FITC | NB21-1021F | |||
| HRP | NB21-1021H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1021SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1074AF405 | |||
| Alexa Fluor 488 | NB21-1074AF488 | |||
| Alexa Fluor 647 | NB21-1074AF647 | |||
| Alexa Fluor 700 | NB21-1074AF700 | |||
| Biotin | NB21-1074B | |||
| DyLight 405 | NB21-1074V | |||
| DyLight 488 | NB21-1074G | |||
| DyLight 550 | NB21-1074R | |||
| DyLight 650 | NB21-1074C | |||
| FITC | NB21-1074F | |||
| HRP | NB21-1074H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1074SS | |||
![Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0013.jpg "Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 uL of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody (No Ab) was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive, and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Chromatin-Immunoprecipitation-NB500-171-img0016.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse testis.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0017.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0020.jpg)
![Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-NB500-171-img0012.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti- used at 1:20,000 and Anti-Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] (NBP2-16848) used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB500-171 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - Immunofluorescence staining to detect histone H3 in human blood. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0023.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 antibody on Mouse pancreas tissue. Histone H3 antibody dilution 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0025.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - (1) HeLa, (2) S. cerevisiae whole cell lysates, (3) Histones purified from HeLa cells. Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0014.jpg)
![Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Observed molecular weight is ~17 kDa. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-171-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody [NB500-171] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB500-171-img0019.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat pancreas.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0009.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat colon.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse brain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunohistochemistry-Paraffin-NB500-171-img0007.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0012.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.")
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9]. Observed molecular weight ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of A549 cells (fixed with methanol) using Histone H3 [Trimethyl Lys9] antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0014.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Used to perform ChIP on HeLa cells.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Chromatin-Immunoprecipitation-NBP1-30141-img0002.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - H3K9Me3 analysis of MCF7 and HCT116. Theorectical molecular weight is ~15 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0008.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0013.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys9-Antibody-6F12-H4-Western-Blot-NBP1-30141-img0004.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Analysis of Histone H3 K4me3 using the peptides listed above in 10, 3, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Dot-Blot-NB21-1023-img0001.jpg "Dot Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Analysis of Histone H3 K4me3 using the peptides listed above in 10, 3, and 1 picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1023 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1023-img0010.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Analysis of Histone H3 K4me3 in C. elegans embryo nuclear extract. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1023-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Histone H3 K4me3 antibody tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1023-img0009.jpg)
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody [NB21-1023] - Analysis of HeLa histone prep (A), NIH 3T3 histone prep (B), HeLa whole cell extract (C), and NIH 3T3 whole cell extract (D) using Histone H3 [Trimethyl Lys4] antibody (NB21-1023) at 1 ug/ml. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys4]-Antibody-Western-Blot-NB21-1023-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-57135-img0004.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - HL60 cells treated with DMSO and Calcimycin Green: (at 1:1000 Blue Hoechst)")
![Western Blot: Histone H3 [Citrulline 2, Citrulline 8, Citrulline 17] Antibody [NB100-57135] - Histone H3/Citrulline (R2,R8,R17) at 1:10000 dilution. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Citrulline-2-Citrulline-8-Citrulline-17]-Antibody-Western-Blot-NB100-57135-img0003.jpg)
![Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of HIstone H3pS10 with picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Dot-Blot-NB21-1091-img0009.jpg "Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of HIstone H3pS10 with picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser10] Antibody [NB21-1091] - IF Confocal analysis of HeLa cells using Histone H3 [p Ser10] antibody (NB21-1091, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1091-img0017.jpg)
![Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of Histone H3pS10 in HeLa histone prep lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Western-Blot-NB21-1091-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of FFPE human testis using Histone H3 [pS10] antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclei staining of Histone H3 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB21-1091-img0015.jpg)
![Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of H3pS10 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Western-Blot-NB21-1091-img0011.jpg)
![Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Dot blot analysis of H3 K4me2 using the peptides stated above in 25, 10, 3 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Dot-Blot-NB21-1022-img0001.jpg "Dot Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Dot blot analysis of H3 K4me2 using the peptides stated above in 25, 10, 3 and 1 picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1022 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1022-img0008.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Western blot analysis of H3 K4me2 in HeLa histone preps at 1:1000. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1022-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - Histone H3 was tested at 1:100 in Hela cells against Dylight 488 (Green). Nuclei were illuminated blue with DAPI.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1022-img0007.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - H3K4Me2 immunoblot in Jurkat cells. Observed molecular weight is ~17 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1022-img0009.jpg)
![Western Blot: Histone H3 [Dimethyl Lys4] Antibody [NB21-1022] - WB analysis of H3 K4me2 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Antibody-Western-Blot-NB21-1022-img0004.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-16840-img0021.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Observed molecular weight is ~15 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16840-img0027.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse intestine. Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Paraffin-NBP2-16840-img0026.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-16840-img0008.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at a dilution of 1:1000. Observed molecular weight is ~15kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-16840-img0014.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Sample (30 ug of whole cell lysate) A: Jurkat, B: Raji, C: K562, D: THP-1, 7.5% SDS PAGE diluted at 1:500. Observed molecular weight is ~19 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-16840-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using HIST1H3A (tri-Methyl K27) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16840-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Immunofluorescence photomicrographs of frozen sections of mouse liver. Red: Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody Blue: Nuclear staining with Hoechst 33342.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunofluorescence-NBP2-16840-img0011.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Hela xenograft, using Histon H3 (tri-Methyl K27) antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Paraffin-NBP2-16840-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse brain. Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Paraffin-NBP2-16840-img0015.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse duodenum. Histone H3K27me3 (trimethyl Lys27) antibody diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Paraffin-NBP2-16840-img0016.jpg)
![Immunohistochemistry Whole-Mount: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Histone H3K27me3 (trimethyl Lys27) antibody dilution: 1:100.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Whole-Mount-NBP2-16840-img0023.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse intestine. Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Paraffin-NBP2-16840-img0025.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody diluted at 1:2500.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Dot-Blot-NBP2-16840-img0012.jpg)
![Chromatin Immunoprecipitation: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NBP2-36468 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Chromatin-Immunoprecipitation-NBP2-36468-img0003.jpg "Chromatin Immunoprecipitation: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NBP2-36468 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.")
![Western Blot: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Western blot image of Anti-Histone H3 antibody (clone 1B1-B2). Whole cell protein from HeLa (lane 1), NIH-3T3 (lane 2) and PC12 (lane 3) were separated by 4-15% SDS-PAGE and transfered to PVDF membrane. The membrane was probed with purified Anti-Histone H3 antibody at 2 ug/ml and detected with an HRP conjugated anti-mouse secondary using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Western-Blot-NBP2-36468-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Immunohistochemistry-Paraffin-NBP2-36468-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody (1B1-B2) [NBP2-36468] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Histone H3 (1B1-B2) NBP2-36468 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Immunocytochemistry-Immunofluorescence-NBP2-36468-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis in Hela cells using siRNA to knockdown Pol II, controls are GAPDH and Histone H3. Theoretical molecular weight of Histone H3 is 15 kDa. Image from a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Western-Blot-NBP2-36468-img0009.jpg)
![Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Dot-Blot-NB21-1024-img0015.jpg "Dot Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac antibody in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 [ac Lys4] Antibody [NB21-1024] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1024 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0019.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in C. elegans embryo lysate. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys4] Antibody [NB21-1024] - Histone H3 K4ac1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-NB21-1024-img0021.jpg)
![Western Blot: Histone H3 [ac Lys4] Antibody [NB21-1024] - Analysis of Histone H3 K4ac in (1) HeLa histone prep and (2) NIH 3T3 histone prep lysates. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys4]-Antibody-Western-Blot-NB21-1024-img0016.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Western-Blot-NB600-1168-img0008.jpg "Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 5 ug/mL Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG, FITC conjugate (green). Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Immunocytochemistry-Immunofluorescence-NB600-1168-img0007.jpg)
![Flow Cytometry: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - FACS profile of human leukemic cells with Anti-phospho-Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Flow-Cytometry-NB600-1168-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0002.jpg "Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of Histone H3K4me1 in HeLa histone prep lysate. Observed molecular weight is ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Histone H3.2 K4me1 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Immunocytochemistry-NB21-1021-img0001.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - H3K4Me1 immunoblot in Jurkat cells. Observed molecular weight is ~17 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0006.jpg)
![Western Blot: Histone H3 [Monomethyl Lys4] Antibody [NB21-1021] - Analysis of H3K4me1 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Antibody-Western-Blot-NB21-1021-img0003.jpg)
![Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Dot-Blot-NB21-1074-img0011.jpg "Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NB21-1074] - 2 ug of NB21-1074 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1074-img0014.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of H3K9ac in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3.2 K9ac antibody was tested at 1:100 in HeLa cells with FITC (green).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1074-img0018.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in HeLa histone prep. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0016.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0012.jpg)